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EC number: 911-418-6 | CAS number: 55965-84-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in soil
Some information in this page has been claimed confidential.
Administrative data
- Endpoint:
- biodegradation in soil: simulation testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 307 (Aerobic and Anaerobic Transformation in Soil)
- Version / remarks:
- OECD Draft Document, April 2000
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- laboratory
Test material
- Test material form:
- not specified
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Rohm and Haas, Batch No. 1063.0008
RADIOLABELLING INFORMATION (if applicable)
- Radiochemical purity: 96.9%
- Specific activity: 48.5 mCi/g
- Locations of the label: 14C label was at the 4 and 5 position
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Not reported
- Stability under test conditions: Half-life in aerobic water:sediment simulation studies range from 0.38 – 1.4 days
- Solubility and stability of the test substance in the solvent/vehicle: Water solubility (deionized water) > 1000 ppm
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: No
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: Dissolution in water
- Final dilution of a dissolved solid, stock liquid or gel: 0.966 ppm 14C-MIT
FORM AS APPLIED IN THE TEST (if different from that of starting material)
Liquid - Radiolabelling:
- yes
Study design
- Oxygen conditions:
- aerobic
- Soil classification:
- USDA (US Department of Agriculture)
- Year:
- 2 006
Soil properties
- Soil no.:
- #1
- Soil type:
- silt loam
- % Clay:
- 26
- % Silt:
- 54
- % Sand:
- 20
- pH:
- 6.3
- CEC:
- 9.6 meq/100 g soil d.w.
- Bulk density (g/cm³):
- 0.99
- Details on soil characteristics:
- SOIL COLLECTION AND STORAGE
- Geographic location: Spring House, Pennsylvania, USA
- Sampling depth (cm): 20
- Soil preparation (e.g., 2 mm sieved; air dried etc.): The soil was sieved through a 2 mm sieve and preconditioned at 20 ± 2°C prior to use
PROPERTIES OF THE SOILS (in addition to defined fields)
- Moisture at 1/3 atm (%): 25.6
- Bulk density (g/cm3): 0.99
Duration of test (contact time)
- Soil No.:
- #1
- Duration:
- <= 100 d
Initial test substance concentration
- Soil No.:
- #1
- Initial conc.:
- 0.966 ppm
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- radiochem. meas.
Experimental conditions
- Soil No.:
- #1
- Temp.:
- 20.1 ± 0.4°C
- Humidity:
- 25.6% at 1/1 Bar
- Microbial biomass:
- 6 x 10e6 cfu/g
- Details on experimental conditions:
- 1. PRELIMINARY EXPERIMENTS:
A total of 28 glass bottles at 250ml were prepared by adding 50 g (dwb) of soil to each. Twelve bottles were dosed at 1 ppm 14C MIT, 12 at 5 ppm, and 4 at 10 ppm. The bottles were sealed with 2-hole stoppers containing glass tubes. The glass tubes of the bottles in series were connected by plastic tubing allowing a vacuum flow through the system which was maintained in a dark incubator at 20 ± 2°C. The flow through system consisted of a bottle with water at the vacuum inlet followed by the bottles containing soil and terminating with volatile traps containing ethylene glycol 1.0N KOH closest to the vacuum source. Samples were taken at 0, 3, 24, 48, and 120 hours for the 1 ppm and 5 ppm dosed samples and at 24 and 120 hours for the 10 ppm dosed samples.
2. EXPERIMENTAL DESIGN
- Soil preincubation conditions (duration, temperature if applicable): 20 ± 2°C
- Soil condition: fresh
- Soil (g/replicate): 50
- Control conditions, if used (present differences from other treatments, i.e., sterile/non-sterile, experimental conditions): Sterilized soil
- No. of replication controls, if used: 2
- No. of replication treatments: 2
- Test apparatus (Type/material/volume): 250ml glass bottles
- Details of traps for CO2 and organic volatile, if any: 50 ml of ethylene glycol (trap for volatile organic compounds) and 50 ml of 1.0N KOH (trap for CO2).
- Identity and concentration of co-solvent: Water
Test material application
- Volume of test solution used/treatment: 102 μl
- Application method (e.g. applied on surface, homogeneous mixing etc.): Applied on surface
- Is the co-solvent evaporated: Not applicable
Any indication of the test material adsorbing to the walls of the test apparatus: No
Experimental conditions (in addition to defined fields)
- Moisture maintenance method: Air flow
- Continuous darkness: Yes/No
3. OXYGEN CONDITIONS (delete elements as appropriate)
- Methods used to create the an/aerobic conditions: Air flow
4. SUPPLEMENTARY EXPERIMENTS:
A second set of soils were dosed at 1, 10, and 25 ppm MIT containing a 1:1 mixture of 14C:13C-MIT and incubated 3 to 120 hours to assist in metabolite identification.
5. SAMPLING DETAILS
- Sampling intervals: Hours 0, 2, 5, 22, 48, and 120 and Days 10, 30, and 100
- Sampling method for soil samples: Whole soil sample was extracted using solvents (methanol:acetonitrile and methanol:KOH )
- Method of collection of CO2 and volatile organic compounds: The presence of 14CO2 in the KOH traps was confirmed by adding BaCl2 to aliquots of the trap solution and the resulting BaCO3 radioassayed
- Sampling intervals/times for:
> Sterility check, if sterile controls are used: Not reported
> Moisture content: At each sample interval
> Redox potential/other: Not reported
> Sample storage before analysis: Not reported
Results and discussion
Material (mass) balance
- Soil No.:
- #1
- % Recovery:
- 100.6
- St. dev.:
- 2.2
% Degradationopen allclose all
- Soil No.:
- #1
- % Degr.:
- 16.8
- Parameter:
- radiochem. meas.
- Sampling time:
- 0 h
- Soil No.:
- #1
- % Degr.:
- 46.5
- Parameter:
- radiochem. meas.
- Sampling time:
- 2 h
- Soil No.:
- #1
- % Degr.:
- 92.7
- Parameter:
- radiochem. meas.
- Sampling time:
- 22 h
- Soil No.:
- #1
- % Degr.:
- 95.1
- Parameter:
- radiochem. meas.
- Sampling time:
- 48 h
- Soil No.:
- #1
- % Degr.:
- 96.1
- Parameter:
- radiochem. meas.
- Sampling time:
- 120 h
- Soil No.:
- #1
- % Degr.:
- 93.2
- Parameter:
- radiochem. meas.
- Sampling time:
- 10 d
- Soil No.:
- #1
- % Degr.:
- 98.1
- Parameter:
- radiochem. meas.
- Sampling time:
- 30 d
- Soil No.:
- #1
- % Degr.:
- 99.3
- Parameter:
- radiochem. meas.
- Sampling time:
- 100 d
Half-life / dissipation time of parent compoundopen allclose all
- Key result
- Soil No.:
- #1
- DT50:
- 21.7 h
- Type:
- (pseudo-)first order (= half-life)
- Temp.:
- 20 °C
- Remarks on result:
- other: 0-22 h
- Soil No.:
- #1
- DT50:
- 1 645 h
- Type:
- second order
- Temp.:
- 20 °C
- Remarks on result:
- other: 0-100 d
- Transformation products:
- yes
Identity of transformation productsopen allclose all
- Details on transformation products:
- - Formation and decline of each transformation product during test: The two main metabolites were present at concentrations greater than 21% at Hour 22 but were transient decreasing to less than 1.3% by Day 100
- Pathways for transformation: Cleavage of the isothiazolone ring with the ultimate metabolite being CO2 - Evaporation of parent compound:
- no
- Volatile metabolites:
- yes
- Remarks:
- Only CO2
- Residues:
- yes
- Details on results:
- TEST CONDITIONS
- Aerobicity (or anaerobicity), moisture, temperature and other experimental conditions maintained throughout the study: Yes
MAJOR TRANSFORMATION PRODUCTS
- Range of maximum concentrations in % of the applied amount and day(s) of incubation when observed:
- Range of maximum concentrations in % of the applied amount at end of study period:
on the - the and -th day of incubation, respectively. At the end of the study period, the corresponding concentrations were - and -- % of the applied amount, respectively.
EXTRACTABLE RESIDUES
- % of applied amount at day 0: 93.7
- % of applied amount at end of study period: 13.3
NON-EXTRACTABLE RESIDUES
- % of applied amount at day 0: 0 6.2
- % of applied amount at end of study period: 38.8
MINERALISATION
- % of applied radioactivity present as CO2 at end of study: 46.6
VOLATILIZATION
- % of the applied radioactivity present as volatile organics at end of study: 0
STERILE TREATMENTS (if used)
- Transformation of the parent compound: Not described
- Formation of transformation products: No
- Formation of extractable and non-extractable residues: No
- Volatilization: 0.2% CO2
Applicant's summary and conclusion
- Conclusions:
- MIT rapidly biodegrades in soil with a half-life of 6.5 hours in a silt loam soil incubated at 20.1 ± 0.4°C. Metabolism involves cleavage of the isothiazolone ring with the ultimate metabolite being CO2.
- Executive summary:
- The test guideline was OECD Draft Document for a New Guideline 307: Aerobic and Anaerobic Transformation in Soil, April 2000. The half-life based on the data from sampling intervals 0- 22 hours is 6.5 hours. Thus, MIT is rapidly biodegraded in soil. The total extractable activity decreased rapidly from 93.7% of the applied activity at Hour 0 to 66.6% at Hour 48 and 13.3% at Day 100. Simultaneous evolved 14CO2 increased from 1.8% of applied activity at Hour 2 to 46.6% on Day 100. Bound residue (post extraction solids) increased from 6.2% of the applied activity at Hour 0 to 39.7% on Day 30. Recovery averaged 100.6 ± 2.2% The decrease in extractable activity and increase in evolved14CO2observed correlated with the decreasing amount of MIT with time. MIT decreased from 83.2% of applied activity at Hour 0 to 37.9% at Hour 5 and 0.7% on Day 100. Five metabolites plus CO2 were detected. Besides CO2, two metabolites, M3 and M4 were predominant; 2-(methylcarbamoyl)-ethene sulfonic acid and 2-(methylcarbamoyl)-1-oxo-ethane sulfinic acid (current data suggests that these are actually the cis and trans isomers of 2-(methylcarbamoyl)-ethene sulfonic acid). M3 and M4 were present at greater than 21% at Hour 22 but were transient decreasing to less than 1.3% by Day 100. M1, M2, and M5 comprised multiple compounds none exceeding 5%. Evolved14CO2consistently increased throughout the study so that it becomes the predominate metabolites (46.6% on Day 100). This indicates that MIT, the metabolites, and the bound residue were biodegrading to CO2.
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