2,5-xylenol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1968

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

The acute toxicity of 4 solid, crystalline xylenol isomers including 2,5-xylenol was studied in white mice, albino rats, and rabbits. A single dose of the substances was administered by mouth in the form of finely dispersed aqueous suspensions. The animals were kept under clinical observation for 15 days. Data on rat and mice mortality were processed statistically by probit analysis as modified by Prozorovsky (1962). LD50 for rabbits was determined by the method of Deichmann and Le Blanc (1943).

GLP compliance:

no

Test type:

other: not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,5-xylenol

EC Number:

202-461-5

EC Name:

2,5-xylenol

Cas Number:

95-87-4

Molecular formula:

C8H10O

IUPAC Name:

2,5-dimethylphenol

Test material form:

solid: crystalline

Test animals

Species:

rat

Strain:

other: albino rats

Sex:

not specified

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

water

Details on oral exposure:

A single dose of 2,5-xylenol was administered by mouth in the form of a finely dispersed aqueous suspension of the solid, cristalline material.

Doses:

not specified

No. of animals per sex per dose:

not specified

Control animals:

not specified

Details on study design:

The acute toxicity of 2,5-xylenol was studied in white mice, albino rats, and rabbits. A single dose of the substance was administered by mouth in the form of a finely dispersed aqueous suspension of the solid, cristalline material. The animals were kept under clinical observation for 15 days.

Statistics:

Mortality data of rats and mice were processed statistically by probit analysis as modified by Prozorovsky; LD50 for rabbits was determined by the method of Deichmann and Le Blanc (1943).

References:

Deichmann W, LeBlanc T (1943) Determination of the approximate lethal dose with about 6 animals. J Ind Hyp Toxicol 25, 415-417.
Prozorovsky VB (1962) Application of a method of least squares for probit-analysis of the lethality curves // Farmakol. i toksikol. - 1962. -N9l. - P. 115-120 (in Russian).

Results and discussion

Effect levels

Key result

Sex:

not specified

Dose descriptor:

LD50

Remarks:

species: rat

Effect level:

ca. 444 mg/kg bw

Based on:

test mat.

Remarks on result:

other:

Remarks:

S:E. 26 mg/kg bw

Mortality:

Most of the animals with acute poisoning died within 24 hours of administration.

Clinical signs:

other: The clinical picture was similar in all the species of laboratory animals investigated (and for all of the 4 isomers considered). The clinical signs of acute poisoning were dyspnea, disturbance of motor coordination, a rapid advent of clonic spasms, and a

Gross pathology:

no data

Any other information on results incl. tables

Species	LD50-values (mg/kg bw ± standard error if indicated)
White mice	383 ± 36
Albino rats	444 ± 26
Rabbits	938

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

2,5-Xylenol showed slight acute oral toxicity, with a rat LD50 value of 444 ± 26 (S.E.) mg per kg body weight.

Executive summary:

The acute toxicity of 4 solid, crystalline xylenol isomers including 2,5-xylenol was studied in white mice, albino rats, and rabbits in accordance with international accepted scientific methods. No guideline was followed. A single dose of the substances was administered by mouth in the form of finely dispersed aqueous suspensions. The animals were kept under clinical observation for 15 days. Data on rat and mice mortality were processed statistically by probit analysis as modified by Prozorovsky (1962).  LD₅₀ for rabbits was determined by the method of Deichmann and Le Blanc (1943). The clinical picture was similar in all the species of laboratory animals investigated (and for all the isomers considered). The clinical signs of acute poisoning were dyspnea, disturbance of motor coordination, a rapid advent of clonic spasms, and an asymmetrical body position. Most of the animals with acute poisoning died within 24 hours of administration.

Based on the study results, LD₅₀values of 383 ±36 (S.E.), 444 ± 26, and 938 mg/kg bw were calculated for white mice, albino rats and rabbits, respectively.