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EC number: 201-317-9 | CAS number: 81-04-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
1,5-Naphthalenedisulfonic acid was investigated using the Salmonella/microsome test for point mutagenic effects in doses of up to 5000 µg per plate on four Salmonella typhimurium LT2 mutants. These comprised the histidine-auxotrophic strains TA 1535, TA 100, TA 1537 and TA 98. Evidence of mutagenic activity of 1,5-naphthalenedisulfonic acid was not seen. No biologically relevant increase in the mutant count, in comparison with the negative controls, was observed. Therefore, 1,5-naphthalenedisulfonic acid was considered to be non-mutagenic without and with S9 mix in the Salmonella/microsome test.
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant study conducted similar to OECD guideline 471 with only minor limitations.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- only four tester strains investigated, limited purity of 77%
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
- Target gene:
- his operon
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor supplemented post-mitochondrial fraction (S9-mix), prepared from the livers of rats treated with Aroclor 1254.
- Test concentrations with justification for top dose:
- Experiment I:
- 8, 40, 200, 1000 and 5000 µg/plate (with and without metabolic activation)
Experiment II:
- 150, 300, 600, 1200, 2400 and 4800 µg/plate (with and without metabolic activation) - Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: without S9-mix: sodium azide, 10 µg/plate (TA 1535); nitrofurantoin, 0.2 µg/plate (TA 100); 4-nitro-1,2-phenylene diamine, 10 µg/plate (TA 1537) and 0.5 µg/plate (TA 98); with S9-mix: 2-aminoanthracene, 3 µg/plate (TA 1535, TA 1537, TA 98 and TA 100)
- Remarks:
- DMSO was used as vehicle for the positive controls
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- No increase in revertant count was observed
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- A significant decrease in bacterial count (titre assessment) was observed ≥200 µg/plate (TA 1535, TA 100, TA 98) and ≥600 µg/plate (TA 1537)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
- Executive summary:
1,5-Naphthalenedisulfonic acid was investigated using the Salmonella/microsome test for point mutagenic effects in doses of up to 5000 µg per plate on four Salmonella typhimurium LT2 mutants. These comprised the histidine-auxotrophic strains TA 1535, TA 100, TA 1537 and TA 98.
Doses of up to and including 150 µg per plate did not cause any bacteriotoxic effects: Total bacteria counts remained unchanged and no inhibition of growth was observed. At higher doses, the substance had a strain-specific bacteriotoxic effect. However, this range could nevertheless be used for assessment purposes.
Evidence of mutagenic activity of 1,5-naphthalenedisulfonic acid was not seen. No biologically relevant increase in the mutant count, in comparison with the negative controls, was observed. Therefore, 1,5-naphthalenedisulfonic acid was considered tobe non-mutagenic without and with S9 mix in the Salmonella/microsome test.
The positive controls sodium azide, nitrofurantoin, 4-nitro- 1,2-phenylene diamine and 2-aminoanthracene had a marked mutagenic effect, as was seen by a biologically relevant in crease in mutant colonies compared to the corresponding negative controls.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Description of key information
No data available
Additional information
The Ames test was performed with the following four Salmonella typhimurium strains: TA 1535, TA 100, TA 1537 and TA 98.
TA 102 or E. coli WP2 was not included in the test. Since the test compound has no oxidizing or cross-linking activities and does not contain a hydrazine substructure no further testing is necessary; see OECD TG 471 paragraph 13. In addition no mutagenic acitivity was seen in a recent Ames test accoring to OECD TG 471 including E.coli WP2 for 1,5-Naphthalenedisulfonic acid, disodium salt monohydrate (unpublished report).
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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