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EC number: 240-642-0 | CAS number: 16587-71-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: read-across from a guideline study
- Justification for type of information:
- Toxicity to microorganisms is based on read-across from p-tert-butylcyclohexanon. The documentation is presented in the Endpoint summary of Aquatic toxicity.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 517 mg/L
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 103 mg/L
- Basis for effect:
- inhibition of total respiration
- Validity criteria fulfilled:
- yes
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 Feb 2002 to 06 Feb 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The substance has been added to about 130 mL deionized water and stirred overnight before testing (equilibration phase) and was subsequently directly weighed into the test vessels.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Type: mixed population of aquatic microorganisms (activated sludge)
- Origin: aeration tank of a waste water treatment plant treating predominantly domestic sewage (Wupper area water authority)
- Pretreatment: aeration of the activated sludge; daily feed with synthetic medium
- pH of the suspension before application: 7.3 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20.7 - 21.6 °C
- pH:
- 7.3 - 8.3
- Dissolved oxygen:
- - Dissolved O2 at start of the test: 5.3 - 7.4 mg/L
- Dissolved O2 at termination of the test: 2.6 - 7.2 mg/L - Nominal and measured concentrations:
- - Nominal concentrations: 0 (control), 320, 560, 1000, 1800 and 3200 mg/L
- Nominal concentrations reference substance: 2.5, 5.0, 10.0, 20.0 and 40.0 mg/L - Details on test conditions:
- TEST SYSTEM
- Aeration: yes, permanent (3 hours)
- No of replicates: not specified
- No vessels per control (replicates): 2
- Test concentration of the activated sludge: 360 mg/L suspended solids (because of strong respiration of the activated sludge).
TEST PARAMETERS MEASURED
- Respiration rate: The respiration rate of an activated sludge is measured after a contact time of 3 hours.
- Other parameters: The oxygen concentration was measured in all vessels at the starting and termination of the test. Furthermore, the pH and temperature were determined. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 517 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 103 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- Inhibition was observed from the lowest test substance concentration (31.7%) and increased in a dose-dependent manner up to 90.7% inhibition at 3200 mg/L. See 'Any other information on results incl. tables'.
- Results with reference substance (positive control):
- The 3-h EC50 value for the reference substance was determined to be 8.2 mg/L. See 'Any other information on results incl. tables'.
- Reported statistics and error estimates:
- Probit analysis
- Validity criteria fulfilled:
- yes
- Remarks:
- See 'Any other information on materials and methods incl. tables'
- Conclusions:
- The 3-h EC50 and EC10 values for microorganisms in activated sludge are 517 and 103 mg/L, respectively.
- Executive summary:
The toxicity of the substance to microorganisms was investigated in an activated sludge respiration inhibition study according to OECD TG 209 and in compliance with GLP criteria. In this study activated sludge from a predominantly domestic sewage was exposed to nominal test substance concentrations of 0 (control), 320, 560, 1000, 1800 and 3200 mg/L for 3 hours. Test concentrations were not analytically verified. The effects on microorganisms was determined by measuring total respiration rates and comparing these to an unexposed control. Inhibition was observed from the lowest test substance concentration (31.7%) and increased in a dose-dependent manner up to 90.7% inhibition at 3200 mg/L. Based on these results, 3-h EC50 and EC10 values of 517 and 103 mg/L were derived, respectively.
Referenceopen allclose all
Table: results test substance / controls
Nominal test concentrations
[mg/L] |
Respiratory rate test substance [mg/L · h] |
Phys.-chem. O2 consumption
[mg/L · h] |
Respiratory rate phys.-chem. O2 consumption [mg/L · h] |
Inhibition
[%] |
320 |
25.0 |
0.0* |
25.0 |
31.7 |
560 |
15.4 |
0.0* |
15.4 |
57.9 |
1000 |
12.0 |
0.0* |
12.0 |
67.2 |
1800 |
4.8 |
0.0* |
4.8 |
86.9 |
3200 |
3.4 |
0.0* |
3.4 |
90.7 |
Control, mean |
36.6 |
|
||
Control 1 |
36.0 |
|
||
Control 2 |
37.2 |
|
* The physico-chemical oxygen consumption has been determined at 10000 mg/L test substance concentration. As no physico-chemical oxygen consumption was observed at that test substance concentration, this observation also holds true for the lower test substance concentrations
Table: results for the reference substance 3,5 -dichlorophenol
Nominal test concentration [mg/L] |
Respiratory rate [mg/l·h] |
Inhibition [%] |
2.5 |
28.8 |
21.3 |
5 |
27.4 |
25.1 |
10 |
15.0 |
59.0 |
20 |
7.2 |
80.3 |
40 |
4.3 |
88.3 |
ADDITIONAL TEST RESULTS TEST SUBSTANCE
- EC25: 221 mg/L
- EC75: 1210 mg/L
- EC90: 2600 mg/L
Description of key information
No data is available for Orivone. Therefore read-across is performed to the close structural analogue p-tert-butylcyclohexanone (CAS 98-53-3). Its toxicity of the substance to microorganisms was investigated in an activated sludge respiration inhibition study according to OECD TG 209 and in compliance with GLP criteria. In this study activated sludge from a predominantly domestic sewage was exposed to nominal test substance concentrations of 0 (control), 320, 560, 1000, 1800 and 3200 mg/L for 3 hours. Test concentrations were not analytically verified. The effects on microorganisms was determined by measuring total respiration rates and comparing these to an unexposed control. Inhibition was observed from the lowest test substance concentration (31.7%) and increased in a dose-dependent manner up to 90.7% inhibition at 3200 mg/L. Based on these results, 3-h EC50 and EC10 values of 517 and 103 mg/L were derived, respectively. There is no need to convert the toxicity of the source chemical data towards Orivone, because of the comparable properties of the substances.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 517 mg/L
- EC10 or NOEC for microorganisms:
- 103 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.