4-hydroxy-3,5-dimethoxybenzaldehyde

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Study period:

2002

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Remarks:

The substance is included in the training set of a QSAR but it is unclear what the source of this endpoint is.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

2002

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The toxicity values were from a population growth impairment test using the ubiquitous freshwater ciliate T. pyriformis (strain GL-C), performed following the protocol previously described by Schultz (1997). The endpoint, population density, of this static 40-h assay was measured spectrophotometrically at 540 nm. Test conditions allow for 8–9 cell cycles in control cultures. Each compound was tested in a range finder prior to testing in duplicate for three definitive replicates. Two controls, one without test chemical but inoculated with T. pyriformis, and the other, a blank with neither test chemical nor ciliates, were used to provide a measure of the test acceptability and as a basis for interpretation of treatment data. Each definitive test replicate consisted of six to eight different concentrations with duplicate flasks of each concentration. Only replicates with con- trol-absorbency values between 0.60 and 0.75 were used in the analyses.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-hydroxy-3,5-dimethoxybenzaldehyde

EC Number:

205-167-5

EC Name:

4-hydroxy-3,5-dimethoxybenzaldehyde

Cas Number:

134-96-3

Molecular formula:

C9H10O4

IUPAC Name:

4-hydroxy-3,5-dimethoxybenzaldehyde

Test material form:

not specified

Details on test material:

Solved in Ethanol. Source: Sigma Chemical Co.

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Tetrahymena pyriformis

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

40 h

Results and discussion

Effect concentrations

Key result

Duration:

40 h

Dose descriptor:

IC50

Effect conc.:

0.001 mol/L

Nominal / measured:

not specified

Basis for effect:

growth inhibition

Applicant's summary and conclusion

Conclusions:

IC50 for Tetrahymena pyriformis was 0.000676 mol/L.