Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 288-538-4 | CAS number: 85750-13-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Skin Irritation:
On the basis of available studies for the structurally similar read across substances, the weight of evidence approach was applied to assess the dermal irritation potential for target substance.4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline was estimated to be not irritating to skin.
Eye Irritation:
On the basis of available studies for the structurally similar read across substances, the weight of evidence approach was applied to assess the ocular irritation potential of 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline.
4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline was estimated to be not irritating to eyes.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Weight of evidence based on structurally similar read across chemicals
- Justification for type of information:
- Weight of evidence based on structurally similar read across chemicals
- Reason / purpose for cross-reference:
- read-across: supporting information
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- other: Weight of evidence based on structurally similar substances
- Principles of method if other than guideline:
- Weight of evidence based on structurally similar substances
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material: 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline
- IUPAC name: 4-[(2-chloro-4-nitrophenyl)diazenyl]-N-ethyl-N-[2-[1-(2-methylpropoxy)ethoxy]ethyl]aniline
- Molecular formula: C22H29ClN4O4
- Molecular weight: 448.9481 g/mole
- Smiles : CCN(CCOC(C)OCC(C)C)c1ccc(cc1)/N=N/c2ccc(cc2Cl)[N+](=O)[O-]
- InChl: 1S/C22H29ClN4O4/c1-5-26(12-13-30-17(4)31-15-16(2)3)19-8-6-18(7-9-19)24-25-22-11-10-20(27(28)29)14-21(22)23/h6-11,14,16-17H,5,12-13,15H2,1-4H3/b25-24+
- Substance type: Organic
- Physical state: Solid crystalline powder (white) - Species:
- rabbit
- Strain:
- New Zealand White
- Type of coverage:
- semiocclusive
- Preparation of test site:
- clipped
- Vehicle:
- water
- Controls:
- not specified
- Amount / concentration applied:
- 500 mg moistened with water
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 72 hours
- Number of animals:
- 3 females
- Details on study design:
- The study is based on weight of evidence approach from the read across values
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- 72 h
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- No signs of irritation observed
- Interpretation of results:
- other: not irritating
- Conclusions:
- On the basis of available studies for the structurally similar read across substances, the weight of evidence approach was applied to assess the dermal irritation potential for target substance.4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline was estimated to be not irritating to skin.
- Executive summary:
Based on the available studies for the structurally similar read across chemicals, weight of evidence approach was applied to assess the dermal irritation potential of 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline.
A study was performed to determine the irritation potential of the structurally similar read across chemical following the procedures outlined in OECD 404.One day prior to the application of the test chemical, the dorsal area of the trunk of 3 female New Zealand White rabbits was clipped free of fur. 500 mg of the test chemical was moistened with water was applied clipped skin of 3 rabbits under semi-occlusive conditions for 4 hours. Subsequently, the dressings were removed, any residual test substance was wiped off. The skin was examined for erythema, eschar formation and edema at 1, 24, 48 and 72 hours after removal of the patches.
No signs of irritation were noted on the rabbit skin. Brown/yellow skin discolouration was observed at the 1-hour time-point but did not prevent the accurate assessment of erythema.
Based on the observations, the test chemical can be considered not irritating to rabbit skin.
The above results were supported by a study performed in accordance with OECD 404 and 92/69/EEC Annex V Guidelines to determine the irritation potential of the structurally similar read across chemical. 0.5 g of the test chemical was moistened with water and applied to 6.25 cm² area of intact skin of 3 female New Zealand albino rabbits under semi-occlusive conditions for 4 hours. Remaining test substance was removed by swabbing with cotton wool swabs soaked in warm water. The skin was examined for erythema, eschar formation and edema at 1, 24, 48 and 72 hours after removal of the patches. An index of Cutaneous Primary Irritation was calculated from the mean scores at the sites and at each time point.
No signs of irritation were noted on the rabbit skin. Red/orange staining was reported at all time points. The primary irritation index was reported to be 0.0.
Based on the scores and observations, the test chemical can be considered not irritating to rabbit skin.
Based on the available data for the structurally similar read across substances and applying the weight of evidence approach, it can be concluded that the target chemical will also tend to behave in a similar manner that of the read across substances. Therefore the target chemical was estimated to be not irritating to skin and it can be further classified under the category “Not Classified” as per CLP regulation.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Weight of evidence based on structurally similar substances
- Justification for type of information:
- Weight of evidence based on structurally similar substances
- Reason / purpose for cross-reference:
- read-across: supporting information
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- other: Weight of evidence based on structurally similar substances
- Principles of method if other than guideline:
- The weight of evidence report has been prepared based on the read across substances identified based on structural and functional similarity to assess the ocular irritation potential of 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material: 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline
- IUPAC name: 4-[(2-chloro-4-nitrophenyl)diazenyl]-N-ethyl-N-[2-[1-(2-methylpropoxy)ethoxy]ethyl]aniline
- Molecular formula: C22H29ClN4O4
- Molecular weight: 448.9481 g/mole
- Smiles : CCN(CCOC(C)OCC(C)C)c1ccc(cc1)/N=N/c2ccc(cc2Cl)[N+](=O)[O-]
- InChl: 1S/C22H29ClN4O4/c1-5-26(12-13-30-17(4)31-15-16(2)3)19-8-6-18(7-9-19)24-25-22-11-10-20(27(28)29)14-21(22)23/h6-11,14,16-17H,5,12-13,15H2,1-4H3/b25-24+
- Substance type: Organic
- Physical state: Solid crystalline powder (white) - Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- no data available
- Vehicle:
- other: undiluted; aqueous saline suspension
- Controls:
- not specified
- Amount / concentration applied:
- 100 mg of the powdered dye; 0.1 ml of 33% aqueous saline suspension
- Duration of treatment / exposure:
- single exposure
- Observation period (in vivo):
- no data available
- Duration of post- treatment incubation (in vitro):
- no data available
- Number of animals or in vitro replicates:
- no data available
- Details on study design:
- The study is based on weight of evidence approach from the read across values
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- other: no data available
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- No signs of irritation observed
- Interpretation of results:
- other: not irritating
- Conclusions:
- On the basis of available studies for the structurally similar read across substances, the weight of evidence approach was applied to assess the ocular irritation potential of 4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline.
4-((2-Chloro-4-nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline was estimated to be not irritating to eyes. - Executive summary:
Based on the available studies for the structurally similar read across chemicals, weight of evidence approach was applied to assess the ocular irritation potential of 4-((2-Chloro-4 -nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline.
The ocular irritation potential of the structurally similar read across chemical was assessed in rabbits. 100 mg of the powdered dye was instilled in one eye of 6 rabbits and the other eye served as control. The treated eyes were observed and scored at 1, 2, 3, and 7 days. The maximum attainable score was 110.
No signs of irritation were observed in the treated eyes of rabbits at any time point.
The scores obtained at 1, 2, 3, and 7 days were 0.0. Hence, the test chemical can be considered to be not irritating to rabbit eyes.
This result is further supported by an eye irritation study performed in rabbits to determine the irritation potential of the structurally similar read across chemical.
0.1 ml of 33% aqueous suspension in saline was instilled in to rabbit eyes and observed for effects (duration, number of rabbits and observation period not mentioned). No signs of irritation were observed. Hence, the test chemical was considered to be not irritating to rabbit eyes.
Based on the available data for the structurally similar read across substances and applying the weight of evidence approach, it can be concluded that the target chemical will also tend to behave in a similar manner that of the read across substances. Therefore the target chemical was estimated to be not irritating to eyes and it can be further classified under the category “Not Classified” as per CLP regulation.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Skin Irritation:
Based on the available studies for the structurally similar read across chemicals, weight of evidence approach was applied to assess the dermal irritation potential of 4-((2-Chloro-4 -nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline.
A study was performed to determine the irritation potential of the structurally similar read across chemical following the procedures outlined in OECD 404.One day prior to the application of the test chemical, the dorsal area of the trunk of 3 female New Zealand White rabbits was clipped free of fur. 500 mg of the test chemical was moistened with water was applied clipped skin of 3 rabbits under semi-occlusive conditions for 4 hours. Subsequently, the dressings were removed, any residual test substance was wiped off. The skin was examined for erythema, eschar formation and edema at 1, 24, 48 and 72 hours after removal of the patches.
No signs of irritation were noted on the rabbit skin. Brown/yellow skin discolouration was observed at the 1-hour time-point but did not prevent the accurate assessment of erythema.
Based on the observations, the test chemical can be considered not irritating to rabbit skin.
The above results were supported by a study performed in accordance with OECD 404 and 92/69/EEC Annex V Guidelines to determine the irritation potential of the structurally similar read across chemical. 0.5 g of the test chemical was moistened with water and applied to 6.25 cm² area of intact skin of 3 female New Zealand albino rabbits under semi-occlusive conditions for 4 hours. Remaining test substance was removed by swabbing with cotton wool swabs soaked in warm water. The skin was examined for erythema, eschar formation and edema at 1, 24, 48 and 72 hours after removal of the patches. An index of Cutaneous Primary Irritation was calculated from the mean scores at the sites and at each time point.
No signs of irritation were noted on the rabbit skin. Red/orange staining was reported at all time points. The primary irritation index was reported to be 0.0.
Based on the scores and observations, the test chemical can be considered not irritating to rabbit skin.
Based on the available data for the structurally similar read across substances and applying the weight of evidence approach, it can be concluded that the target chemical will also tend to behave in a similar manner that of the read across substances. Therefore the target chemical was estimated to be not irritating to skin and it can be further classified under the category “Not Classified” as per CLP regulation.
Eye Irritation:
Based on the available studies for the structurally similar read across chemicals, weight of evidence approach was applied to assess the ocular irritation potential of 4-((2-Chloro-4 -nitrophenyl)azo)-N-ethyl-N-(2-(1-(2-methylpropoxy)ethoxy)ethyl)aniline.
The ocular irritation potential of the structurally similar read across chemical was assessed in rabbits. 100 mg of the powdered dye was instilled in one eye of 6 rabbits and the other eye served as control. The treated eyes were observed and scored at 1, 2, 3, and 7 days. The maximum attainable score was 110.
No signs of irritation were observed in the treated eyes of rabbits at any time point.
The scores obtained at 1, 2, 3, and 7 days were 0.0. Hence, the test chemical can be considered to be not irritating to rabbit eyes.
This result is further supported by an eye irritation study performed in rabbits to determine the irritation potential of the structurally similar read across chemical.
0.1 ml of 33% aqueous suspension in saline was instilled in to rabbit eyes and observed for effects (duration, number of rabbits and observation period not mentioned). No signs of irritation were observed. Hence, the test chemical was considered to be not irritating to rabbit eyes.
Based on the available data for the structurally similar read across substances and applying the weight of evidence approach, it can be concluded that the target chemical will also tend to behave in a similar manner that of the read across substances. Therefore the target chemical was estimated to be not irritating to eyes and it can be further classified under the category “Not Classified” as per CLP regulation.
Justification for classification or non-classification
The results of the experimental studies from the structurally similar read across substances indicate a possibility that 4-[(2-chloro-4-nitrophenyl)diazenyl]-N-ethyl-N-[2-[1-(2-methylpropoxy)ethoxy]ethyl]aniline can be not irritating to skin and eyes.
Hence by applying the weight of evidence approach, 4-[(2-chloro-4-nitrophenyl)diazenyl]-N-ethyl-N-[2-[1-(2-methylpropoxy)ethoxy]ethyl]aniline can be considered to be not irritating to skin and eyes. It can be classified under the category “Not Classified” as per CLP regulation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.