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EC number: 247-092-0 | CAS number: 25549-16-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-07-18 to 2016-11-21
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was added in the required amounts according to the test concentrations directly to the test vessels.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: wastewater treatment plant of Mannheim, Germany
- Preparation of inoculum for exposure: After arrival of the activated sludge suspension in the test facility the suspension was sieved with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over night at room temperature. At the next day the sludge suspension was washed once with drinking water and the suspension was adjusted to 3 g/L dw.
- Initial biomass concentration: 1.5 g/L dw - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 19.7 - 20.5 °C
- pH:
- 7.3 - 7.5
- Dissolved oxygen:
- > 7 mg/L
- Nominal and measured concentrations:
- test substance, nominal: 62.5; 125; 250; 500; 1000 mg/L
reference substance, nominal: 1, 10, 100 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glas-beakers (nominal volume 1L)
- Aeration: yes
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per concentration of reference substance (replicates): 2
- Sludge concentration (weight of dry solids per volume): 1.5 g/L dw
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic OECD medium
OTHER TEST CONDITIONS
- Adjustment of pH: not needed
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Oxygen uptake for a period of 8 to 10 minutes
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: oxygen consumption
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: oxygen consumption
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: no
- Adsorption: none
- Blank controls oxygen uptake rate: 21 mg/g x h
- Coefficient of variation of oxygen uptake rate in control replicates: 3.9 % O2 consumption - Results with reference substance (positive control):
- - Results with reference substance: valid
- Relevant effect levels: EC50 = 2 - 25 mg/L - Validity criteria fulfilled:
- yes
Reference
Table 1 Measurement of the oxygen consumption for blank control (BC) and reference substance (RS)
Assay identification |
BC1 |
BC2 |
BC3 |
BC4 |
BC5 |
BC6 |
RS1 |
RS2 |
RS3 |
RS4 |
RS5 |
RS6 |
O2 conc. Start [mg/L] |
7.5 |
7.0 |
8.0 |
8.0 |
8.3 |
8.0 |
8.0 |
7.5 |
8.4 |
8.5 |
9.0 |
8.8 |
O2 conc. End [mg/L] |
4.5 |
4.0 |
5.0 |
5.0 |
5.3 |
4.5 |
5.4 |
5.0 |
6.8 |
6.9 |
8.6 |
8.4 |
O2 consumption rate [mg/L] |
3.0 |
3.0 |
3.0 |
3.0 |
3.0 |
3.5 |
2.6 |
2.5 |
1.6 |
1.6 |
0.4 |
0.4 |
O2 consumption in minutes |
6.0 |
6.0 |
6.1 |
5.9 |
6.0 |
6.3 |
5.8 |
6.0 |
6.0 |
6.2 |
6.0 |
6.0 |
O2 consumption rate [mg/L x h], RT |
30 |
30 |
30 |
31 |
30 |
33 |
27 |
25 |
16 |
15 |
4 |
4 |
Specific O2 consumption rate [mg/gxh] |
20 |
20 |
20 |
21 |
20 |
22 |
18 |
17 |
11 |
10 |
3 |
3 |
Calculation of inhibition respiration [%], IT |
- |
12 |
18 |
48 |
51 |
87 |
87 |
Table 2 Measurement of the oxygen consumption for Test Substanc (TS)
Assay identification |
TS1 |
TS2 |
TS3 |
TS4 |
TS5 |
TS6 |
TS7 |
TS8 |
TS9 |
TS10 |
TS11 |
TS12 |
TS13 |
TS14 |
TS15 |
O2 conc. Start [mg/L] |
7.5 |
7.4 |
8.0 |
7.1 |
7.0 |
7.2 |
7.0 |
7.0 |
6.0 |
7.0 |
8.0 |
6.5 |
7.0 |
7.6 |
7.0 |
O2 conc. End [mg/L] |
4.5 |
4.5 |
5.2 |
4.1 |
4.0 |
4.0 |
3.6 |
3.8 |
2.7 |
4.0 |
5.2 |
3.5 |
4.5 |
5.0 |
4.0 |
O2 consumption rate [mg/L] |
6.1 |
6.0 |
6.0 |
6.0 |
6.0 |
6.0 |
6.1 |
6.0 |
6.0 |
6.2 |
6.1 |
6.1 |
6.0 |
5.9 |
6.0 |
O2 consumption in minutes |
3.0 |
2.9 |
2.8 |
3.0 |
3.0 |
3.2 |
3.4 |
3.2 |
3.3 |
3.0 |
2.8 |
3.0 |
2.5 |
2.6 |
3.0 |
O2 consumption rate [mg/L x h], RT |
30 |
29 |
28 |
30 |
30 |
32 |
33 |
32 |
33 |
29 |
28 |
30 |
25 |
26 |
30 |
Specific O2 consumption rate [mg/gxh] |
20 |
19 |
19 |
20 |
20 |
21 |
22 |
21 |
22 |
19 |
19 |
20 |
17 |
17 |
20 |
Calculation of inhibition respiration [%], IT |
2 |
5 |
9 |
2 |
2 |
-4 |
-8 |
-4 |
-8 |
5 |
9 |
2 |
18 |
15 |
2 |
Legend: RS = reference substance, BC = blank control, TS = test substance
The respiration rate was calculated in mg O2/L×h. The oxygen consumption was measured over a period of 8-10 minutes. Specifically, 5.8 - 6.3 minutes (RS and BC), 6 - 6.1 minutes (TS1-9) or 5.9 – 6.2 minutes (TS10-15) from the plotted track within a linear range of the graph was used for the determination of the respiration rate.
Description of key information
EC50 for microorganisms was determined to be greater than 1000 mg/L in an activated sludge study, conforming to GLP and OECD 209 guideline.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
Activated sludge study
An activated sludge respiration inhibition test, according to GLP and OECD 209 was performed.
The 3 hours effect concentrations for the test item are EC10, EC20, EC50, and EC80 > 1000 mg/L. No monotone concentration response exists. Therefore, the ECx calculation is not meaningful and the EC10 value is assumed to be greater than 1000 mg/L. The results in this study are consistent with all validity criteria and the test is valid according to the guidelines used for this study.
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