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EC number: 259-653-7 | CAS number: 55466-76-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07-Nov-95 to 19-Dec-95
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Guideline study (OECD 471), to GLP, using five Salmonella strains. None of the strains utilised has the capacity to detect certain types of mutagens (e.g. oxidising or cross-linking agents). The results of this study are supplemented by those of the study reported by Parnham (1996), which utilised such a strain (E.coli).
Cross-reference
- Reason / purpose for cross-reference:
- reference to other study
Reference
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18-Sep-1996 to 11-Oct-1996
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Study does not stand alone, since only one bacterial strain was utilised, but it provides supplementary data of a comparable reliability to that in another study (Thompson, 1996), which utilised five Salmonella strains.
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- (only one bacterial strain used)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
- Target gene:
- Tryptophan
- Species / strain / cell type:
- E. coli WP2 uvr A
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 from Aroclor 1254-induced male rat liver
- Test concentrations with justification for top dose:
- 0, 50, 150, 500, 1500 and 5000 ug/plate
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- -S9
- Positive control substance:
- other: N-ethyl-N'-nitro-N-nitrosoguanidine, 2 ug/plate
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- +S9
- Positive control substance:
- other: 2-aminoanthracene, 10 ug/plate
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
- Conclusions:
- Ruthenium acetate was not mutagenic in a GLP bacterial reverse mutation assay in Escherichia coli WP2 uvrA, at up to 5 mg/plate, with and without S9.
- Executive summary:
The mutagenicity of ruthenium acetate (CAS 55466-76-7) was evaluated in an bacterial reverse mutation assay using a single strain of Escherichia coli WP2 uvrA, following a protocol similar to OECD guideline 471 and to GLP. The bacteria were exposed to the test material at concentrations of up to 5 mg/plate in the presence and absence of an exogenous metabolising system (S9). No increase in the number of revertants was seen at any concentration either with or without S9. The test material was not mutagenic under the conditions of the test. This bacterial strain is utilised for its capacity to detect certain types of mutagen, such as cross-linking agents, not detected by Salmonella typhimurium strains TA98, TA100, TA1535, TA1537 or TA1538. The results of this study supplement those of the study reported by Thompson (1996), which omitted such a strain.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Remarks:
- (but no strain capable of detecting cross-linking mutagens was included)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- Remarks:
- (but no strain capable of detecting cross-linking mutagens was included)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Hexakis[μ-(acetato-O:O')]-μ3-oxo-triangulo-triruthenium acetate
- EC Number:
- 259-653-7
- EC Name:
- Hexakis[μ-(acetato-O:O')]-μ3-oxo-triangulo-triruthenium acetate
- Cas Number:
- 55466-76-7
- Molecular formula:
- C12H18O13Ru3.C2H3O2
- IUPAC Name:
- hexamethyl-2λ³-oxa-4λ³-oxa-6λ³-oxa-8λ³-oxa-10λ³-oxa-12λ³-oxa-13λ³-oxa-15λ³-oxa-16λ³-oxa-18λ³-oxa-19λ³-oxa-21λ³,22λ¹-dioxa-1,5,9-triruthenahexacyclo[7.3.3.3¹,⁵.3⁵,⁹.1¹,⁵.0⁹,²²]docosa-3,7,11,14,17,20-hexaene-1,1,1,5,5,5,9,9,9-nonakis(ylium)-2,6,10,13,16,19-hexaide-22,22-diuide acetate
- Details on test material:
- - Name of test material (as cited in study report): ruthenium acetate
- Substance type: black crystalline solid
- Physical state: solid
- Analytical purity: not stated
- Impurities (identity and concentrations): not stated
- Composition of test material, percentage of components: not stated
- Isomers composition: not stated
- Purity test date: not stated
- Lot/batch No.: 60350
- Expiration date of the lot/batch: not stated
- Stability under test conditions: not stated
- Storage condition of test material: room temperature
- Other:
- Date received: 23-Oct-1995
Constituent 1
Method
- Target gene:
- histidine
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- S. typhimurium TA 1538
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 from Aroclor 1254-induced male rat liver
- Test concentrations with justification for top dose:
- 0, 50, 150, 500, 1500 and 5000 ug/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: water
- Justification for choice of solvent/vehicle: not stated, but well-known solvent
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- for TA100 and TA1535 -S9
- Positive control substance:
- other: N-ethyl-N'-nitro-N-nitrosoguanidine, 3 and 5 ug/plate
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- for TA1537 -S9
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Migrated to IUCLID6: 80 ug/plate
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- for TA98 -S9
- Positive control substance:
- 4-nitroquinoline-N-oxide
- Remarks:
- Migrated to IUCLID6: 0.2 ug/plate
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- for TA1538 -S9
- Positive control substance:
- other: 4-Nitro-o-phenylenediamine, 5 ug/plate
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- water
- True negative controls:
- no
- Positive controls:
- yes
- Remarks:
- 5 for all strains +S9
- Positive control substance:
- other: 2-aminoantracene, 0.5-2 ug/plate
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 48 hours
NUMBER OF REPLICATIONS: In triplicate
DETERMINATION OF CYTOTOXICITY
- Method: other: thinning of the background lawn
- Evaluation criteria:
- For a substance to be considered positive, it would have induced a concentration-related and statistically significant increase in mutation rate in at least one strain in the presence and/or absence of S9 in both experiments at sub-toxic concentrations; to be considered negative, the number of induced revertants at each concentration would be less than 2x the number of spontaneous revertants.
- Statistics:
- Using the methods recommended by the United Kingdom Environmental Mutagen Society (UKEMS), normally Dunnett's method of linear regression.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
TEST-SPECIFIC CONFOUNDING FACTORS: none
RANGE-FINDING/SCREENING STUDIES: yes, preliminary toxicity study using TA100 exposed to the test material at 5000 ug/plate
COMPARISON WITH HISTORICAL CONTROL DATA: yes, within historical control range for the test laboratory
ADDITIONAL INFORMATION ON CYTOTOXICITY: no cytotoxicity- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Ruthenium acetate was not mutagenic in a guideline Ames assay with five strains of Salmonella typhimurium, when tested at up to 5 mg/plate, with and without S9.
- Executive summary:
The mutagenicity of ruthenium acetate (CAS 55466-76-7) was evaluated in an Ames test, conducted according to OECD Test Guideline 471 and to GLP. Five strains of bacteria (Salmonella typhimurium TA 1535, TA 1537, TA 1538, TA 98 and TA 100) were exposed to the test material at concentrations up to 5 mg/plate, in the presence and absence of an exogenous metabolising system (S9). No increase in the number of revertants was seen in any strain at any concentration either with or without S9. The test material was not mutagenic under the conditions of the test.
None of the strains utilised has the capacity to detect certain types of mutagens (e.g. oxidising or cross-linking agents). The results of this study are supplemented by those of the study reported by Parnham (1996), which utilised such a strain.
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