1,3,4-Thiadiazole-2(3H)-thione, 5-(tert-dodecyldithio)-

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1978

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

not applicable

GLP compliance:

no

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

1,3,4-Thiadiazole-2(3H)-thione, 5-(tert-dodecyldithio)-

EC Number:

813-543-0

Cas Number:

73984-93-7

Molecular formula:

C14H26N2S4

IUPAC Name:

1,3,4-Thiadiazole-2(3H)-thione, 5-(tert-dodecyldithio)-

Test material form:

liquid

Test animals

Species:

rat

Strain:

Crj: CD(SD)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River
- Age at study initiation: not reported
- Weight at study initiation: 264 to 284g (males) and 169 to 182g (females)
- Housing: individually in wire-mesh cages
- Diet (e.g. ad libitum): Purina laboratory chow was available ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 1 week

ENVIRONMENTAL CONDITIONS
- Temperature (°C): during the 4 h exposure period the temperature in the exposure chamber was 27°C
- Humidity (%): not reported
- Air changes (per hr): not reported
- Photoperiod (hrs dark / hrs light): not reported

IN-LIFE DATES:
From: 15 Dec 1978 To: 29 Dec 1978

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

The vapours of the compound were generated by passing air at the rate of 10 L/min through a round bottom flask containing the compound. The flask was placed in a heating jacket heated to 93°C. The vapours emerging from the flask were introduced into the exposure chamber without dilution. The temperature within the exposure chamber was approximately 27° C. The rats were thus exposed to an atmosphere essentially saturated with vapours of the test material. The concentration of the vapour (0.62 mg/L) in the chamber atmosphere was calculated from the ratio of the total weight loss of the material in the flask after the exposure (1.49 grams) to the total volume of air passing through the flask during the 4 hour period (2400 litres).

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

4 h

Concentrations:

One exposure level of 0.62 mg/L was used

No. of animals per sex per dose:

5 males and 5 females were used

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations were performed twice daily during the 14 day observation period and body weights were performed at Day 0 (prior to exposure), 1, 3, 5 ,7 and 14
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 0.62 mg/L air (nominal)

Based on:

test mat.

Exp. duration:

4 h

Mortality:

No mortality occured during the study

Clinical signs:

other: The immediate response of the rats to the experimental atmosphere was an increase of activity in preening. After several minutes of exposure this activity decreased. The rats appeared normal during the 4 hour exposure and throughout the 14 day observati

Body weight:

No effect on body weight was noted during the study, refer to table below.

Gross pathology:

No findings noted

Any other information on results incl. tables

Animal No.	Body weight (g)
	Day 0	Day 1	Day 3	Day 5	Day 7	Day 14
M9356 M9357 M9358 M9359 M9360	278 282 276 264 284	280 283 275 259 278	286 280 277 260 280	289 277 279 259 280	286 283 285 265 284	288 287 296 275 284
F9361 F9362 F9363 F9364 F9365	182 172 177 178 169	182 168 173 180 169	180 167 173 180 168	177 167 168 175 165	182 168 171 178 168	182 174 170 178 169

Applicant's summary and conclusion

Interpretation of results:

study cannot be used for classification

Remarks:

Migrated information

Executive summary:

An acute inhalation study was conducted using a similar procedure to that described in the current OECD Guideline 403.

The test material (CAS No. 50530 -43 -3) was administered at 0.62 mg/L by inhalation for 4 h to five male and five female rats. The rats were observed for 14 days after test material administration. No mortalities occurred during the study. No gross pathologic alterations were noted among the animals.

The acute inhalation median lethal dose (LD50) was estimated to be greater than 0.62 mg/L.