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EC number: 700-150-3 | CAS number: 156572-81-5
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 83.9
- Sampling time:
- 28 d
- Remarks on result:
- other: mean of 3 replicates
- Interpretation of results:
- readily biodegradable
- Conclusions:
- In a study performed according to OECD 301B, SLMI is readily biodegradable (83.9% in 28d) and passes the 10 day window.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge : Brazos River Authority Sewage Treatment Plant Sugar Land, TX
- Storage conditions: Sample was aerated during transport and upon arrival at the laboratory - Duration of test (contact time):
- ca. 28 d
- Initial conc.:
- >= 10 - <= 20 mg/L
- Based on:
- other: TOC/L
- Details on study design:
- TEST CONDITIONS
- Composition of medium: 1 L using deionised water 33.0 g Na2HPO4 2H2O, 8.5g KH2PO4, 21.75g K2HPO4, 0.5g NH4Cl, 36.4g CaCl 2H2O, 22.5g MgSO4 7H2O, 0.25 Fe Cl3 6H2O
- Additional substrate:
- Test temperature: 20-25º C
- pH: 7.4±0.5
- pH adjusted: yes
- Aeration of dilution water: CO2 free air was pumped throgh the flasks continually
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Glass flasks
- Number of culture flasks/concentration: 2 flasks for each blank, teference and test flask. (8 flasks total)
- Method used to create aerobic conditions: CO2 free air was pumped continously through the flasks
- Test performed in open system: No
- Details of trap for CO2 : 160 ml square bottles containing 100 ml of 0.0125 M Ba(OH)2 were attached to the test flasks.
SAMPLING
- Sampling frequency: Samples were taken on days 2, 3, 4, 5, 6, 7, 8, 9, 11, 12, 14, 15, 19, 25, 26, and 27.
- Sampling method: The Ba(OH)2 was titrated against 0.05NHCl to measure the amount of CO2 evolved. on Day 27 ll test flasks were acidified using NHCl The flasks were then aerated for a further 24 hours. All remaining Ba(OH)2 was then removed for titration on day 28.
- Reference substance:
- aniline
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 90.4
- Sampling time:
- 28 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- ca. 69.34
- Sampling time:
- 12 d
- Remarks on result:
- other: This was the end of the 10 day window started on day 2. As SCMI produced CO2 equal to more than 60% of its CO2 then it can be considered readily biodegradable
- Results with reference substance:
- The results for distilled aniline showed that on day 15 the mean biodegradation was 73.52% therefore the test was considered valid.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
Referenceopen allclose all
Description of key information
In a study performed according to OECD 301B, SLMI is readily biodegradable and passes the 10 day window.
In a study on read-across substance
SCMI (Rodrigue, 2007) showed Ready Biodegradability passing the 10 day
window.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Two key studies have been performed to test the ready biodegradability following the OECD 301B CO2 evolution test methods. The first study was performed on SLMI and showed biodegradability of >80% in 28days and passed the 10 day window.
The second study was performed on the read-across substance SCMI (Rodrigue. 2007) and measured biodegradation of >60% at the end of 10 days, showing ready biodegradation of the substance.
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