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Diss Factsheets
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EC number: 695-735-2 | CAS number: 68489-14-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- The study was conducted using a full range of concentrations as toxicity was observed at the threshold level.
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Water samples were taken from the control and all surviving test groups at 0 and 72 hours from fresh media, and at 24 and 96 hours from old media for quantitative analysis.
- Vehicle:
- no
- Details on test solutions:
- The test solution were prepared using a prlonged stir method. An initial loading rate of 100 mg/L was stirred for a period of 24 hours prior to the removal of any undissolved test item by filtration through a 0.2 micrometer Sartorius Sartopore filter.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Fish were obtained from Brow Well Fisheries Limited and maintained in house from the 7th October 2015. Fish were maintained in a glass fibre tank with single pass water renewal system. Fish were acclimatised to the test conditions from the 19th October to 26 October 2015.
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- Total hardness of approximately 140 mg/L as CaCO3.
- Test temperature:
- The water temperature was controlled at approximately at 13 °C.
- pH:
- pH was recorded daily it ranged from 7.5-8.1
- Dissolved oxygen:
- The dissolved oxygen content of was greater or equal to 9.2 mg O2/L
- Nominal and measured concentrations:
- Nominal concentrations of 1, 3.2, 10, 32, and 100 mg/L were used. The test solution was renewed daily.
- Details on test conditions:
- 25-30 litre glass exposure vessels containing 20 litres of test media was used for each test concentration and control. The test vessels were covered to reduce evaporation and maintained at approximately 13 °C in a temperature-controlled room with a photoperiod of 16 hours light and 8 hours darkness with a 20-minute dawn and dusk transition periods for a period of 96 hours. Test vessel were aerated via narrow bore glass tubes. The fish received no food during exposure. a semi static test regime was employed involving a daily renewal of the test preparation to prevent a build-up of nitrogenous waste products.
- Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 5.7 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Details on results:
- Analysis of freshly prepared test preparations at 0 and 72 hours showed measured test concentrations to be near nominal. There was no significant change in the measured concentrations at 24 and 96 hours. The results are therefore based on nominal test concentrations.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 h LC50 was determined to be 5.7 mg/L.
- Executive summary:
The short-term toxicity of the test item to fish was determined following the methods in the OECD 203 guidelines. The test system was a semi-static daily renewal system. The concentration of the test solution was verified analytically at 0 and 72 hours for the fresh media and 24 and 96 hours for the old media. The study found the LC50 of the test item to O. mykiss to be 5.7 mg/L.
Reference
Description of key information
The study found the 96h LC50 of the test item to O. mykis to be 5.7 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 5.7 mg/L
Additional information
The short term toxicity of the test item to fish was determined following the methods in the OECD 203 guidelines. The test system was a semi-static daily renewal system. The concentration of the test solution was verified analytically at 0 and 72 hours for the fresh media and 24 and 96 hours for the old media. The study found the 96 h LC50 of the test item to O. mykis to be 5.7 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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