4,4'-azobis[4-cyanovaleric] acid

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4,4'-azobis[4-cyanovaleric] acid

EC Number:

220-135-0

EC Name:

4,4'-azobis[4-cyanovaleric] acid

Cas Number:

2638-94-0

Molecular formula:

C12H16N4O4

IUPAC Name:

4,4'-azobis[4-cyanovaleric] acid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Analysis of the test media samples was conducted on fresh media at 0 hours and on
corresponding old media at 96 hours. Measured test concentrations were determined
from samples of control and test solution.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Healthy Danio rerio (zebrafish) were supplied by London University College, United
Kingdom, details of which are maintained in Smithers Viscient (ESG) Ltd. records.
The fish supplied came from the same batch of stock fish and were approximately
2 months old when used for the test.
The fish were acclimatised to the test conditions for at least 12 days prior to testing.
The mortality
rate of the stock batch of fish was 0 % in the seven days prior to the definitive test.
Fish were selected, at random, from the holding tank and allocated to the test vessels at
the start of each phase of testing. The fish were not fed for at least 24 hours before the
start of the definitive test and were not fed throughout the test.
At the end of the test, the total length and wet weight of a sub-sample of ten fish were
determined, selected at random from those used in the test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness as CaCO3 (mg/L) 63

Test temperature:

23.9-24.5 °C

pH:

7.18-7.61

Dissolved oxygen:

87-104%

Salinity:

Alkalinity as CaCO3 (mg/L) 119

Conductivity:

235-262 (μS/cm)

Nominal and measured concentrations:

measured concentrations remained
within ± 20 % (109 – 90.2 %) of the nominal concentration

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 123 mg/L

Nominal / measured:

meas. (not specified)

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes