1-chlorododecane

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles.

Data source

Reference

Reference Type:

publication

Title:

Acute toxicity to Daphnia pulex of six classes of chemical compounds potentially hazardous to Great Lakes aquatic biota

Author:

Smith, S. B.; Savino, J. F.; Blouin, M. A.

Year:

1988

Bibliographic source:

J. Great Lakes Res. 14 (4): 394-404

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

other:

Version / remarks:

American Society of Testing and Materials 1980. E729-80

Deviations:

not specified

Test guideline 2

Qualifier:

according to guideline

Guideline:

other:

Version / remarks:

US EPA 1975. Report No. 660/3-75-009

Deviations:

not specified

Principles of method if other than guideline:

the test has been conducted in accordance to the standards of American Society of Testing and Materials (1980) No. E729-80 and US EPA (1975) Report No. 660/3-75-009.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-chlorododecane

EC Number:

203-981-5

EC Name:

1-chlorododecane

Cas Number:

112-52-7

Molecular formula:

C12H25Cl

IUPAC Name:

1-chlorododecane

Specific details on test material used for the study:

CAS No. 112-52-7
Mol Wt. 205

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

not specified

Details on test solutions:

Solvent control is mentioned (acetone), but not specified if used for substance
Reconstituted hard water.

Test organisms

Test organisms (species):

Daphnia pulex

Details on test organisms:

Daphnia pulex cultured in reconstituted hard water at 20°C fed with a mix of green algae species.
Less than 24 h old
no feeding during test.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

total hardness 160-180 mg/L as CaCO3

Dissolved oxygen:

dissolved oxygen concentration 8-9 mg/L

Nominal and measured concentrations:

five concentrations, range not further specified

Details on test conditions:

TEST SYSTEM
- Test vessel: beakers in water bath at 20°C
- No. of organisms per vessel: min. 10 Neonates/ test vessel
- Control and solvent control

EFFECT PARAMETERS MEASURED: immobilisation after 48 h
VEHICLE CONTROL PERFORMED: not specified

Reference substance (positive control):

yes

Remarks:

p,p' DDT

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

0.013 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other:

Remarks:

+/- SE 0.0019 mg/L

Results with reference substance (positive control):

DDT showed an EC50 of 0.0011 mg/L after 48 h.

Reported statistics and error estimates:

EC50 value were calculated by probit analysis and analysis of variance (ANOVA).

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The EC50 of the test substance has been determined to be 0.013 mg/L and has been considered as very highly toxic for invertebrates in comparison to other alkyl halides.