Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 944-962-8 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Samples of the test solutions were collected at approximately 0 and 72 hours to measure concentrations of the test substance. Samples at test initiation were collected from the individual batches of test solution prepared for each treatment and control group prior to distribution into the test chambers and prior to inoculation. At exposure termination, samples were collected from the pooled replicates from each treatment and control group. At each sampling interval, 20 mL of test solution was collected from mid-depth and transferred into glass scintillation vials. Samples were acidified with concentrated phosphoric acid upon collection.
- Vehicle:
- no
- Details on test solutions:
- A primary stock was prepared by dissolving 0.0118 g of the test substance in 1000 mL of freshwater AAP medium to achieve a nominal concentration of 10 mg a.i./L. The primary stock was inverted at least twenty times and stirred for five minutes prior to use and continued stirring while all subsequent dilutions were made. The primary stock appeared clear and colorless. Five additional test solutions were prepared at nominal concentrations of 0.10, 0.26, 0.64, 1.6, and 4.0 mg a.i./L by diluting aliquots of the 10 mg a.i./L primary stock with freshwater AAP medium. The 0.10, 0.26, 0.64, 1.6, and 4.0 mg a.i./L test solutions were mixed by inversion and appeared clear and colorless and were otherwise unremarkable at the time of preparation. The negative control solution consisted of freshwater AAP medium without test substance added.
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Original algal cultures were obtained from the University of Texas at Austin, and have been maintained in culture medium at EAG Laboratories, Easton, Maryland since June 2017. Algal cells used in this test were obtained from EAG Laboratories – Easton cultures that had been actively growing in culture medium under similar environmental conditions as used in this test for at least two weeks prior to test initiation. Algal cells for this study were taken from a culture that had been transferred to fresh medium four days prior to test initiation.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- According to AAP medium
- Test temperature:
- 23.8 - 24.15 °C
- pH:
- Day 0: 7.3 - 7.4
Day 3: 7.4 - 9.8 - Salinity:
- According to AAP medium
- Conductivity:
- According to AAP medium
- Nominal and measured concentrations:
- nominal concentrations: 0.10, 0.26, 0.64, 1.6, 4.0 mg and 10 mg a.i./L
geometric mean, measured concentrations were determined to be 0.050, 0.15, 0.40, 1.2, 3.6, and 9.0 mg a.i/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: 250mL glass erlenmeyers plugged with foam stoppers
- Initial cells density: 10000 / mL
- Control end cells density: haemocytometer
- No. of vessels per concentration (replicates):3
- No. of vessels per control (replicates):6
GROWTH MEDIUM
- Standard medium used: yes AAP
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: purified well water
- Total organic carbon, Metals, Pesticides, Chlorine: checked December 7 , 2016
- Intervals of water quality measurement: not specified
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: yes
- Photoperiod:no
- Light intensity and quality: cool-white fluorescent 6,000 lux ± 10%.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter (Coulter Electronics, Inc.)
- Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.8 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.81 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.05 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth rate
- Details on results:
- After 72 hours of exposure, inhibition of cell density in the 0.050, 0.15, 0.40, 1.2, 3.6, and 9.0 mg a.i./L treatment groups (based on geometric mean, measured concentrations) was 0, 19, 37, 82, 99, and 100%, respectively, relative to the negative control. Inhibition of growth rate in the 0.050, 0.15, 0.40, 1.2, 3.6, and 9.0 mg a.i./L treatment groups was 0, 4, 8, 30, 85, and 100%, respectively, relative to the negative control.
After 72 hours of exposure, adherence of cells to the test chambers was not observed in any of the control or treatment groups. Flocculation or aggregation of cells was not observed in any of the experimental groups. Cells in the 3.6 and 9.0 mg a.i./L treatment groups appeared enlarged when compared to cells in the negative control. Cells present in all other treatment groups appeared normal when compared to cells in the negative control. - Reported statistics and error estimates:
- Dunnett’s test indicated mean growth rate and mean yield were significantly reduced (p < 0.05) in the 0.15, 0.40, 1.2, 3.6, and 9.0 mg a.i./L treatment groups when compared to the negative control. Dunnett’s test indicated mean cell density was significantly reduced (p < 0.05) in the 0.40, 1.2, 3.6, and 9.0 mg a.i./L treatment groups when compared to the negative control. The 72-hour NOEC was determined to be 0.050 mg a.i./L based on statistically significant reductions in growth rate and yield. The 72-hour NOEC for cell density was determined to be 0.15 mg a.i./L. The 72 hour EC50 values for cell density, growth rate, and yield were determined to be 0.52, 1.8, and 0.53 mg a.i./L, respectively.
- Validity criteria fulfilled:
- yes
- Remarks:
- Mean cell density control flasks increased by 318, 3 days, achieving the 16X growth criterion. CV av sp growth rates in control 0.76%,< 7% criterion. mean percent CV section-by-section specific growth rates in control replicates 30.9% < 35% criterion
- Conclusions:
- Toxicity of 1,3-Butylene Glycol Diacrylate to freshwater alga Pseudokirschneriella subcapitata (a.k.a. Raphidocelis subcapitata) was assessed using OECD Test Guideline 201. Growth rate inhibition was evaluated as being ErC50-72h = 1.8 mg/L and ErC10-72h = 0.81 mg/L, meaning that the substance can be classified as toxic to algae according to GHS rules.
- Executive summary:
In agreement with the requirements od OECD TG 201, the freshwater alga, Raphidocelis subcapitata, was exposed to a series of six treatment levels of 1,3-Butylene Glycol Diacrylate ranging from 0.050 to 9.0 mg a.i./L, based on geometric mean, measured concentrations. The toxicity of 1,3-Butylene Glycol Diacrylate to Raphidocelis subcapitata was assessed based on effects on cell density, growth rate, and yield relative to the negative control. The 72-hour NOEC was determined to be 0.050 mg a.i./L based on statistically significant reductions in growth rate and yield. The 72-hour NOEC based on cell density was 0.15 mg a.i./L. The 72-hour EC10values for cell density, growth rate, and yield were determined to be 0.16, 0.81, and 0.18 mg a.i./L, respectively. The 72-hour EC50values for cell density, growth rate, and yield were determined to be 0.52, 1.8, and 0.53 mg a.i./L, respectively.
Reference
Description of key information
Toxicity of 1,3-Butylene Glycol Diacrylate to freshwater alga Pseudokirschneriella subcapitata (a.k.a. Raphidocelis subcapitata) was assessed using OECD Test Guideline 201. Growth rate inhibition was evaluated as being ErC50-72h = 1.8 mg/L and ErC10-72h = 0.81 mg/L, meaning that the substance can be classified as toxic to algae according to GHS rules.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.8 mg/L
- EC10 or NOEC for freshwater algae:
- 0.81 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.