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EC number: 235-979-5 | CAS number: 13078-36-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Publised December 1984
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- The source substance (EDTA 2Na) and the target substance (DTPA 3Na) are the disodium and trisodium salts of their respective aminopolycarboxylic acids, and are therefore structurally similar. The purity/impurity profile of the source substance is not reported in the source publication (Arch. Hydrobiol. Suppl.67, 4, 479-492, December 1984), but since the target material is > 99.9% pure and contains no detectable impurities, the extrapolation of short-term aquatic toxicity properties from the source material to the target material is considered valid as a ‘worst case' scenario. There were no published test guidelines followed nor indication of GLP. However, the study was well conducted and documented, according to generally accepted scientific principles, and was considered reliable with restrictions (Category 2).
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The action of EDTA in batch cultures of Scenedesmus quadricauda at concentrations of 100, 200 and 400 mg/L and the effects of manipulation of macro- and micro-elements concentrations were investigated.
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Specified as EDTA, Na2EDTA.2H2O in the published paper
- Analytical monitoring:
- yes
- Details on sampling:
- Every 24 hours for the first three days and generally less frequently thereafter.
- Vehicle:
- no
- Test organisms (species):
- Scenedesmus quadricauda
- Details on test organisms:
- Scenedesmus quadricauda (TURP.) BREB., strain GREIFSWALD/15 from the institute of Botany, Czechoslovak Academy of Science, Trebon.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 23 d
- Hardness:
- Not reported
- Test temperature:
- 25 +/- 0.1 ºC
- pH:
- 6.8 (adjuted with Sodium hydroxide
- Dissolved oxygen:
- The algal suspensions were bubbled with a mixture of air and 1.5% carbon dioxide
- Salinity:
- Not applicable
- Conductivity:
- No data
- Nominal and measured concentrations:
- Nominal concentrations 100, 200 and 400 mg/L EDTA
Achieved concentrations of EDTA were measured at each time of algal sampling, on Days: 0,1,2,5,6,7,8,9,12,13,15,19, 21and 23. - Details on test conditions:
- The algal culture was precultivated in a medium corresponding to 1/10 of the concentrations used in the study medium. The precultivated culture was then filtered through a filter paper (SCHLEICHER & SCHULL-blue ribbon), the cells were washed twice by repeated resuspension in freshly distilled water and filtration, and finally resuspended again in a small volume of distilled water to give a thick suspension. An adequate quantity of this suspension was then used for the inoculation of each test flask to the initial dry mass concentration of approximately 0.2 g/L.
All of the culture media were prepared in freshly distilled water and not sterilised. The algal cultures were grown in 2-1 ROUX flasks (material SIAL, Czechoslovakia) in an apparatus for batch cultivation. The flasks were continuously illuminated from one side only with either fluorescent tubes (TESLA 40 W neural light) or high pressure sodium discharge lamps (TESLA SHA 400). The irradiance of the algal cultures was 57 Wm3 in the case of the fluorescent tubes and 330Wm3 in the case of the sodium discharge lamps.
1g samples of the cultures were taken on Days: 0,1,2,5,6,7,8,9,12,13, 15,19,21and 23 for determination of cell number (microscopically in a BURKER counting chamber), dry mass (5ml duplicates filtered and dried at 105 ºC) and chlorophyll a + b (disintegration, extraction with acetone and spectrophotometry). - Reference substance (positive control):
- no
- Key result
- Duration:
- 23 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 23 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Chlorophyll concentration
- Details on results:
- Higher concentrations of EDTA (Na2EDTA.2H2O; 400mg/L) when in surplus over trace elements in the nutrient solution, inhibit cell division, chlorophyll synthesis and the production of algal biomass, especially in the earlier phase of algal growth. No negative influence of the higher concentrations of EDTA was observed when the concentration of the trace elements in the nutrient material was increased correspondingly to the increased EDTA concentration. EDTA depletion from the solutions was observed in the experiments where a surplus of EDTA was accompanied by a corresponding surplus of trace elements. The highest decrease in EDTA concentration corresponded to the highest rate of cell division. The influence of the light source on the decomposition of EDTA in the algal nutrient solution was also studied, but no decomposition of EDTA by the light source was proved.
- Validity criteria fulfilled:
- not applicable
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- The source substance (EDTA 2Na) and the target substance (DTPN 3Na) are the disodium and trisodium salts of their respective aminopolycarboxylic acids (Ethylene diaminetetraacetic acid and Diethylenetriaminepentaacetic acid), and are therefore structurally similar. The structural and chemical similarities of EDTA and DTPA have been acknowledged in EU risk management analysis (RMOA for DTPA salts, Dec. 2014). The two substances have high water solubility and would be fully dissociated in the aquatic environment. The target substance is neutral (pH 7.5) in solution which is comparable to the test conditions used for the toxicity study to aquatic algae with EDTA 2Na (pH 6.8). The organic acid moieties of the respective substances have a similar chelating mode of action and would be expected to exert long-term adverse effects by the sequestration of essential metal ions, rather than specific acute toxicity. The extrapolation of aquatic toxicity potential from the source to the target material is therefore considered valid.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 400 mg/L
- Nominal / measured:
- nominal
- Conclusions:
- The NOEC of DTPA trisodium salt to algae is predicted to be greater than 400 mg/L.
- Executive summary:
Using a read-across approach from a study conducted on the structural analogue EDTA 2Na, the NOEC of DTPA trisodium salt to algae is predicted to be greater than 400 mg/l (on stoichiometric basis). The two substances are structurally similar, particularly when ionised in the aqueous environment. The lack of effect at this concentration is dependent on the balance of nutrients to avoid nutritional limitations caused by the sequestrating mechanism of action of the two substances.
Referenceopen allclose all
Description of key information
Using a read-across approach from a study conducted on the structural analogue EDTA, the NOEC of DTPA trisodium salt to algae is predicted to be greater than 400 mg/l (on stoichiometric basis). The two substances are structurally similar, particularly when ionised in the aqueous environment. The lack of effect at this concentration is dependent on the balance of nutrients to avoid nutritional limitations caused by the sequestrating mechanism of action of the two substances. The structural and chemical similarities of EDTA and DTPA have been acknowledged in EU risk management analysis (RMOA for DTPA salts, Dec. 2014).
Key value for chemical safety assessment
- EC10 or NOEC for freshwater algae:
- 400 mg/L
Additional information
Higher concentrations of EDTA (Na2EDTA.2H2O; 400mg/L) when in surplus over trace elements in the nutrient solution, inhibit cell division, chlorophyll synthesis and the production of algal biomass, especially in the earlier phase of algal growth. No negative influence of the higher concentrations of EDTA was observed when the concentration of the trace elements in the nutrient material was increased correspondingly to the increased EDTA concentration. EDTA depletion from the solutions was observed in the experiments where a surplus of EDTA was accompanied by a corresponding surplus of trace elements. The highest decrease in EDTA concentration corresponded to the highest rate of cell division. The influence of the light source on the decomposition of EDTA in the algal nutrient solution was also studied, but no decomposition of EDTA by the light source was proved.
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