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EC number: 214-478-5 | CAS number: 1132-61-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2003-09-03 to 2003-12-04
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- adopted July 17th 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- adopted July 31st 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling was carried out at the start and end of study (0 and 96 h).
- Vehicle:
- no
- Details on test solutions:
- Good quality drinking water is used. The composition is analyzed yearly by the supplier.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Danio rerio
- Strain: Hamilton-Buchanan
- Source: weiler's pet's best, Neustadt, Germany
- Age at study initiation: sexually immature young fish
- Length at study initiation: 2 ± 1 cm
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as during the main study
- Amount of food during acclimation: 1 – 2 % body weight per day
- Feeding frequency during acclimation: 3 times/day
FEEDING DURING TEST
- Food type: No food was provided during the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- no specified
- Test temperature:
- 23 ± 1 °C
- pH:
- 6.7 - 8.6 (no adjustment)
- Dissolved oxygen:
- 7.9 - 9.2 mg/L
- Salinity:
- not applicable
- Conductivity:
- not specified
- Nominal and measured concentrations:
- Nominal concentration: 1000 mg/L
Measured concentration: 106.26 % of the nominal concentration - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass aquaria, maximal volume 10 L
- Aeration: yes (with glass tubes; 1 bubble/sec)
- No. of organisms per vessel: 7 (main test); 3 (pre-test)
- No. of vessels per concentration: 1
- No. of vessels per control: 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12 hours using neon tubes
EFFECT PARAMETERS MEASURED: mortality and abnormal behaviour
TEST CONCENTRATIONS
- Range finding study: A range finding study (pre-test) was conducted.
- Test concentrations: 1000 mg/L
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: No test item related effects observed.
- Observations on body length and weight: none was observed
- Other biological observations: none was observed
- Mortality of control: No mortality was detected in the control.
- Other adverse effects control: no
- Abnormal responses: no
- Any observations that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: no - Sublethal observations / clinical signs:
VALIDITY
- The mortality in the control may not exceed 10 % at the end of the test. No mortalities occurred in the control.
- The concentration of dissolved oxygen must be at least 60 % throughout the test. The concentration of dissolved oxygen stayed above 7.9 mg/L or 92 % throughout the test (100 % at 101300 Pa equals 8.57 mg/L, following DIN 38408 part 22).
ANALYTICAL DETERMINATIONS
The analytical determinations of the test item in the test solution showed good recovery after 96 hours. The recovery of more than 100 % was probably caused by evaporation effects. Therefore the test item can be considered as stable under the test conditions.
RESULTS
Parameter
Value
95 % confidence-interval
24 - 96 h LC0
1000 mg/L
n.a.
24 - 96 h LC50
> 1000 mg/L
n.a.
24 - 96 h LC100
>> 1000 mg/L
n.a.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 96 h LC50 was determined to be > 1000 mg/L and 96 h LC0 was determined to be 1000 mg/L.
- Executive summary:
The objective of the study was to evaluate the acute toxicity of the test item on Zebrafish (Danio rerio) according to OECD TG 203 under GLP conditions. For this purpose, young fish were exposed to aqueous test media containing the test item for 96 h. Based on results obtained in a preliminary experiment fish were exposed to a single nominal concentration of 1000 mg/L (limit test). The test medium served as the negative control. A static test was performed as the test item was previously shown to be stable in the test medium over the test period. The analytical determinations of the test item in the test solution showed good recovery after 96 hours. The recovery of more than 100 % was probably caused by evaporation effects. Thus, the test item can be considered as stable under the test conditions. The nominal concentration was used to estimate the result. The 96 h LC50 for the test item was determined to be > 1000 mg/L and 96 h LC0 was determined to be 1000 mg/L.
Reference
Description of key information
The 96 h LC50 was determined to be > 1000 mg/L (reference 6.1.1-1).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1 000 mg/L
Additional information
The objective of the study was to evaluate the acute toxicity of the test item on Zebrafish (Danio rerio) according to OECD TG 203 under GLP conditions. For this purpose, young fish were exposed to aqueous test media containing the test item for 96 h. Based on results obtained in a preliminary experiment fish were exposed to a single nominal concentration of 1000 mg/L (limit test). The test medium served as the negative control. A static test was performed as the test item was previously shown to be stable in the test medium over the test period. The analytical determinations of the test item in the test solution showed good recovery after 96 hours. The recovery of more than 100 % was probably caused by evaporation effects. Thus, the test item can be considered as stable under the test conditions. The nominal concentration was used to estimate the result. The 96 h LC50 for the test item was determined to be > 1000 mg/L and 96 h LC0 was determined to be 1000 mg/L.
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