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EC number: 257-440-3 | CAS number: 51812-80-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Adopted April 13, 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3-(D-gluconoylamino)propyl(2-hydroxyethyl)dimethylammonium chloride
- EC Number:
- 257-440-3
- EC Name:
- 3-(D-gluconoylamino)propyl(2-hydroxyethyl)dimethylammonium chloride
- Cas Number:
- 51812-80-7
- Molecular formula:
- C13H29N2O7.Cl
- IUPAC Name:
- 1-Propanaminium, 3-(D-gluconoylamino)-N-(2-hydroxyethyl)-N,N-dimethyl-, chloride
- Test material form:
- liquid: viscous
- Details on test material:
- CR Test item 207516/A
Identification Quaternium-22
Appearance Brown paste (determined by Charles River Den Bosch)
Batch 0001954895
Purity/Composition > 90%
Test item storage In refrigerator (2-8°C)
Stable under storage conditions until 16 June 2017 (retest date)
Constituent 1
- Specific details on test material used for the study:
- Test item 207516/A
Identification Quaternium-22
Appearance Brown paste (determined by Charles River Den Bosch)
Batch 0001954895
Purity/Composition > 90%
Test item storage In refrigerator (2-8°C)
Stable under storage conditions until 16 June 2017 (retest date)
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Frequency at t=0 h and t=48 h
Volume 2.0 mL from the approximate centre of the test vessels
Storage Not applicable, samples were transferred to the analytical laboratory at the Test Facility and analysed on the day of sampling.
At the end of the exposure period, the replicates were pooled at each concentration before sampling.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The batch of Quaternium-22 tested was a brown paste with a purity of > 90% and completely soluble in test medium at the concentrations tested. No correction was made for the purity/composition of the test item. Preparation of test solutions started with the highest concentration of 100 mg/L applying a 19- minute period of magnetic stirring to accelerate the dissolution of the test item in medium. Lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. All test solutions were clear and colorless at the end of the preparation procedure.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Source In-house laboratory culture with a known history.
Reason for selection This system has been selected as an internationally accepted invertebrate species.
Validity of batch Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress such as mortality >20%, presence of males, ephippia or discoloured animals and there was no delay in the production of the first brood.
Characteristics For the test selection of young daphnids with an age of < 24 hours, from parental daphnids of more than two weeks old.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/L expressed as CaCO3
- Test temperature:
- 20°C
- pH:
- 7.6 - 8.1
- Dissolved oxygen:
- > 8.0 mg/L
- Nominal and measured concentrations:
- 0.10, 1.0, 10 and 100 mg/L
- Details on test conditions:
- Number of daphnids: 20 each in the control and the highest test concentration; 10 each per intermediate test concentration.
Test vessels: 100 mL, all-glass
Loading: 5 per vessel containing 80 mL of test solution
Light: 16 hours photoperiod daily
Feeding: No feeding
Aeration: No aeration of the test solutions.
Measurements and Recordings:
- Immobility (including mortality):At 24 hours and at 48 hours.
- Dissolved oxygen & pH: At the beginning and at the end of the test, for the highest concentration and the control.
- Temperature of medium: Continuously in a temperature control vessel, beginning at the start of the test. - Reference substance (positive control):
- yes
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- For the determination of the effect parameters in this study, only the highest exposure concentration proved to be of relevance since no biological effects were observed and the test was judged to be a limit test. Since the average exposure concentrations for both peaks were at the level of nominal (106 and 109% for m/z191.2 and 325.2, respectively), the effect parameters were expressed in terms of analytically confirmed nominal concentrations.
No effects were recorded at any of the concentrations tested during the exposure period.
Any other information on results incl. tables
Number of Introduced Daphnids and Incidence of Immobility:
Time (h) |
Replicate |
Quaternium-22; Nominal conc. (mg/L) |
||||
Control |
0.10 |
1.0 |
10 |
100 |
||
0 |
A |
5 |
5 |
5 |
5 |
5 |
B |
5 |
5 |
5 |
5 |
5 |
|
C |
5 |
|
|
|
5 |
|
D |
5 |
|
|
|
5 |
|
Total introduced |
20 |
10 |
10 |
10 |
20 |
|
24 |
A |
0 |
0 |
0 |
0 |
0 |
B |
0 |
0 |
0 |
0 |
0 |
|
C |
0 |
|
|
|
0 |
|
D |
0 |
|
|
|
0 |
|
Total immobilised |
0 |
0 |
0 |
0 |
0 |
|
Effect % |
0 |
0 |
0 |
0 |
0 |
|
|
|
|
|
|
||
48 |
A |
0 |
0 |
0 |
0 |
0 |
B |
0 |
0 |
0 |
0 |
0 |
|
C |
0 |
|
|
|
0 |
|
D |
0 |
|
|
|
0 |
|
Total immobilised |
0 |
0 |
0 |
0 |
0 |
|
Effect % |
0 |
0 |
0 |
0 |
0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion, the 48h-EC50 was beyond the range tested, i.e. exceeded an analytically confirmed nominal concentration of 100 mg/L.
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