Decahydro-2-naphthyl acetate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24 October 2000 - 26 October 2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: The stock solution was analysed at 0 hours, the control was analysed at 0 and 48 hours, and test concentrations were analysed at 0, 24 and 48 hours

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution was prepared to give the desired series of test concentrations. 105.9 mg of the test substance were added to 2 litres of dilution water, treated with an ultra turrax for 60 seconds and afterwards stirred for 24 h an a magnetic stirrer.
- Controls: Dilution water
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): Finally undissolved particles of the test substance were removed by filtration.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna Straus
- Age of parental stock: Parthenogenetic females of synchronised age
- Feeding during test: None

ACCLIMATION
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Unicellular green algae (Scenedesmus subspicatus CHODAT)
- Feeding frequency: ad libitum

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: The neonates were separated from their parent Daphnia by filtration prior to the acute test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

271.3 mg/1 CaCO3

Test temperature:

19.9 - 20.2°C

pH:

7.7 - 7.8

Dissolved oxygen:

8.2 - 8.8 mg/L

Nominal and measured concentrations:

Nominal concentrations 1.25, 2.5, 5, 10, 20 and 36 mg/L
Initial measured concentrations 1.2, 2.4, 5.6, 9.8, 21 and 35 mg/L
Measured concentrations at 48 hours 1.3, 2.6, 7, 8.4, 23.8 and 37.8* mg/L
* Measured after 24 hours of exposure because of early 100% immobilisation rates of the daphnids

Details on test conditions:

TEST SYSTEM
- Test vessel: Karlsruher Flaschen
- Type: closed
- Material, size, headspace, fill volume: 250 to 300 mL test medium per vessel
- Aeration: None
- No. of organisms per vessel: 10 neonates per vessel
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4 medium
- Intervals of water quality measurement: Temperature, pH and dissolved oxygen measured at 0 and 48 hours

OTHER TEST CONDITIONS
- Photoperiod: 16h light: 8h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Substance-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates were observed at 24 and 48 hours.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

other: EC0/EC100

Effect conc.:

7.5 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

2.5 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Remarks:

Calculated (corresponding to the analytical recovery rate of the highest test concentration) as the concentration to be measured was below the quantitation limit of the TOC method (2 mg/L)

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

22.4 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

other: EC0/EC100

Effect conc.:

13.4 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

36 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Measured concentrations: 97.2 — 112% of nominal values at 0 hours, 84 — 140% of nominal values at 48 hours
- Mortality of control: No mortalities occurred throughout the duration of the study

Reported statistics and error estimates:

In those cases where the slope of the concentration/percentage response curve is too steep to permit calculation of the EC50, this value is estimated graphically.

Validity criteria fulfilled:

yes

Remarks:

Immobilisation in the control was < 10% by the end of the test, the dissolved oxygen concentration was >3 mg/L throughout the exposure period, and the concentration of the test substance was within 80% of the initial concentration throughout the study.

Conclusions:

The 48 hour EC0/EC100 was determined to be 7.5 mg/L for Daphnia magna exposed to the test item.

Executive summary:

Short-term toxicity of the test item to Daphnia magna was evaluation in a 48 -hour static test. Daphnia were exposed to nominal test item concentrations of 1.25, 2.5, 5, 10, 20 and 36 mg/L in a closed system, alongisde a dilution water control. Measured test concentrations were 97.2 to 112% of nominal values at 0 hours and 84 to 140% of nominal values at 48 hours based on TOC analysis. The 48-hour EC0/EC100 was determined to be 7.5 mg/L, based on geometric mean measured concentrations. The study was GLP-compliant and was conducted according to EU Method C.2. The study is reliable with restrictions (Klimisch 2) as there were some limitations in the reporting of methods and results.

Description of key information

The 48 hour EC50 was determined to be 7.5 mg/L for Daphnia magna exposed to the test item.
    
    
    
    
    
    
    

The 48-hour EC0/EC100 was determined to be 7.5 mg/L, based on geometric mean measured concentrations.
















 
    
    
  
  
  
  
  
  
  
  

The 48-hour EC0/EC100 was determined to be 7.5 mg/L, based on geometric mean measured conce
























trations.


















ons.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

7.5 mg/L

Additional information

Short-term toxicity of the test item to Daphnia magna was evaluated in a 48 -hour static test (2000). Daphnia were exposed to nominal test item concentrations of 1.25, 2.5, 5, 10, 20 and 36 mg/L in a closed system, alongisde a dilution water control. Measured test concentrations were 97.2 to 112% of nominal values at 0 hours and 84 to 140% of nominal values at 48 hours based on TOC analysis. The 48-hour EC0/EC100 was determined to be 7.5 mg/L, based on geometric mean measured concentrations. The study was GLP-compliant and was conducted according to EU Method C.2. The study is reliable with restrictions (Klimisch 2) as there were some limitations in the reporting of methods and results.