3-cyano-3,5,5-trimethylcyclohexanone

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991-03-11 to 1991-03-16

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Principles of method if other than guideline:

Method: other: OECD Guideline 203 (1984)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-cyano-3,5,5-trimethylcyclohexanone

EC Number:

411-490-4

EC Name:

3-cyano-3,5,5-trimethylcyclohexanone

Cas Number:

7027-11-4

Molecular formula:

C10H15NO

IUPAC Name:

3-cyano-3,5,5-trimethylcyclohexanone

Details on test material:

Test substance: Isophorone nitrile of Degussa AG. Batch No. 64-II/78/90. Purity 99.6 % (GC).

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Dispersion:  640 mg test substance was dissolved in 2 l dilution water. 48, 84, 150,  262.5, 480, and 840 ml thereof were diluted in the test beakers 
to 1.5 l  with dilution water.  960 mg test substance was dissolved in 3 l dilution water for the  highest test concentration (320 mg/l). 

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISMS
- Supplier: M.B. Ruysbroek B.V., Maassluis (The Netherlands)
- Wild caught: No
- Age/size/weight/loading: Length 2.2 +/- 0.1 cm; weight 0.08 +/- 0.01 g
- Feeding during test: No

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

- Test temperature: 24.3 - 25.7 °C

pH:

- pH: 8.0-8.2 (fresh); 7.7-8.1 (spent) (determined at beginning and end of use of each solution)

Dissolved oxygen:

- Dissolved oxygen: 6.8 -  8.2 mg/l (determined at beginning and end of use of each solution)

Nominal and measured concentrations:

- Concentrations: 0; 10; 18; 32; 56; 100; 180; 320 mg/l nominal

Details on test conditions:

DILUTION WATER
- Source: DSWL prepared from ground water from a locality near Linschoten  (The Netherlands). Analytical data: 1.33 mmol/l Na+; 0.24 mmol/l K+;
1.43 mmol/l Ca++; 0.74 mmol/l Mg++; 2.79 mmol/l Cl-; 0.74 mmol/l SO4--  
- Aeration: Slight aeration during test
- Hardness: 217 mg/l as CaCO3
- TOC: 1.5 mg/l - pH: 8.0-8.5

TEST SYSTEM
- Renewal of test solution: Daily, freshly prepared
- Exposure vessel type: 2000 ml all-glass beakers with 1500 ml test  solution
- Number of replicates, fish per replicate: 2 replicates with 10 fish each

TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light / 8 h dark cycle

TEST PARAMETER: 
Mortality, swimming behavior, color, respiratory  function, other observable morphological or visual behavioral criteria,  all observed at least after 24, 48, 72, and 96 hours

STATISTICAL ANALYSIS
- LC50 values were calculated using a parametric moded developed by Kooijman

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

18 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

73 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 56-95 mg/L

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

320 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

LC50 (24 h) = 80 (61-104) mg/l   
LC50 (48, 72, and 96 h) = 73 (56-95) mg/l
- Concentration / response curve: Slope = 0.57 (0.40-0.75)
- Effect concentration vs. test substance solubility: The test substance  appeared to be completely dissolved at all concentrations throughout the  test

Nature of adverse effects: 
- 10 and 18 mg/l: condition of all fish equal to that of the control fish  (0-96h).   
- 32 mg/l: Transiently (48 h) disturbed equilibrium in all fish alive.    
- 56 mg/l: Transiently disturbed equilibrium (24-48 h) and swimming near  the surface (48 h) of all fish alive.   
- 100 mg/l: Disturbed equilibrium (24-96 h) and swimming near the surface (48-96 h) of most fish alive.   
- 180 mg/l: Disturbed equilibrium (24-96 h), swimming near the surface  (48-96 h), and slow swimming (96 h) of all fish alive.   
- 320 mg/l: All fish dead at 24 hour inspection.

Any other information on results incl. tables

Sublethal observations / clinical signs:

- Effect data (Mortality): 
------------------------------------------------
  Concentration  24 h     48 h    72 h     96 h
------------------------------------------------
    0 mg/l         0        0       0        0
   10 mg/l         0        0       0        0
   18 mg/l         0        0       0        0
   32 mg/l         3        4       4        4
   56 mg/l        11       13      13       13
  100 mg/l        13       13      13       13
  180 mg/l        11       11      11       11
  320 mg/l        20       20      20       20
------------------------------------------------
  

Applicant's summary and conclusion

Conclusions:

The LC50 (96 hours) was determined to be 73 mg/L (CL: 56-95 mg/L) indicating that the test substance may be harmful to fish.

Executive summary:

The acute toxicity of isophorone nitrile on the fresh-water fish Brachydanio rerio was determined according to OECD TG 203 by TNO (1991). The test was carried out as a semi-static test with daily renewal of the the test solutions over a period of 96 hours. Seven concentrations ranging from 10 to 320 mg/L plus controls were tested . The following endpoints were derived:

LC50 (96 hours): 73 mg/L (CL: 56 -95 mg/L)

NOEC (96 hours): 18 mg/L

The study was assessed as "reliable without restrictions".