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EC number: 235-243-3 | CAS number: 12138-09-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001-12-11, 2002-02-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
-Source and lot.batch No. of test material: 16031/01
-Purity test date: >98% - Analytical monitoring:
- yes
- Vehicle:
- no
- Details on test solutions:
- Stock Solution: The solution (600 mg/L test item were weighed out) was applied to 6 L test medium (8 parts with 75 mg/ 750 mL)
Dispersion Treatment: The stock solution was shaken with 20 upm for 24 h on a magnetic stirrer and filtrated with 0.45 um
Limit Concentration: Saturated Solution (saturated concentration) - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Danio rerio (zebrafish)
-Origin: Aquarium AM Aegi, FeldstraBe 7, D-30159 Hannover
ACCLIMATION
-Acclimation period: 12days
-Acclimation conditions (same as test or not): 22 degree celcius and diffuse light, water was changed once per week
-Type and amount of food: Trouvit 40/2; Milkivit, D-86666 Burgheim. 4% of the fish body weight feeding per day
- Feeding Frequency: 3 times per week - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Number of Zebrafish incapable of swimming after 24, 48, 72, and 96 h
- Hardness:
- Total hardness of 40 to 180 mg CaC)3/L
- Test temperature:
- 22 +\- 2 degree C
- pH:
- 6.0-8.5
- Dissolved oxygen:
- not less than 80% of air saturation value
- Nominal and measured concentrations:
- The mean measured concentration was 360 ug/L for the test item Tungsten Disulphide
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass-aquaria loosely covered by glass tops
- Material, size, headspace, fill volume: 5 L per vessel
- No. of organisms per vessel: 7 zebrafish for concentration and cotrol
- No. of vesseles per concentration (replicates): one per concentration and control
RANGE-FINDING STUDY? - Reference substance (positive control):
- yes
- Remarks:
- 7 zebrafish in dilution water (without test item)
- Key result
- Duration:
- 2 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 360 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 360 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 360 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 360 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 360 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test concentration of 360 ug/L (mean measured concentration) caused no mortality or non lethal effects. Therefore the NOEC was laid down as 360 ug/L (mean measured concentration)
- Executive summary:
There is short-term toxicity to fish data available for tungsten disulphide.The acute toxicity of the test item tungsten disulphide to fish (Zebrafish) was determined according to EEC Directive 92/69/C1. A saturated solution was tested in a static limit test as single test concentration selected on the basis of a preliminary range finding test. Duration of the test was 96 h. 7 test organisms were exposed to the test concentration and the control and recorded at every 2, 24, 48, 72, and 96 hour. Concentration control analysis was carried out and reported by an external GLP laboratory. Tungsten contents were found to be stable during the test period. The mean measure concentration was 360 ug tungsten/L
The LC-Values with 95% confidence intervals are: EC50 at 2 h: >360 ug/L, EC50 at 24h: >360 ug/L, EC50 at 48h: >360 ug/L, EC50 at 72h: >360 ug/L, and EC50 at 96h: >360 ug/L
On the basis of observations made during the test, the NOEC was determined at 360 ug/L for the test item tungsten disulphide. In this study tungsten disulphide was not found to be toxic to zebrafish after 96h at the mean measured concentration of 360 ug/L
Reference
Description of key information
There is short-term toxicity to fish data available for tungsten disulphide. The acute toxicity of the test item tungsten disulphide to fish (Zebrafish) was determined according to EEC Directive 92/69/C1. A saturated solution was tested in a static limit test as single test concentration selected on the basis of a preliminary range finding test. Duration of the test was 96 h. 7 test organisms were exposed to the test concentration and the control and recorded at every 2, 24, 48, 72, and 96 hour. Concentration control analysis was carried out and reported by an external GLP laboratory. Tungsten contents were found to be stable during the test period. The mean measure concentration was 360 ug tungsten/L
The LC-Values with 95% confidence intervals are: EC50 at 2 h: >360 ug/L, EC50 at 24h: >360 ug/L, EC50 at 48h: >360 ug/L, EC50 at 72h: >360 ug/L, and EC50 at 96h: >360 ug/L
On the basis of observations made during the test, the NOEC was determined at 360 ug/L for the test item tungsten disulphide. In this study tungsten disulphide was not found to be toxic to zebrafish after 96h at the mean measured concentration of 360 ug/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 360 µg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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