3,4,5,6,7,8,9,10,11,12,13,14-dodecahydro-2H-cyclododeca[b]pyran

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Between 1999-04-01 and 1999-04-29

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Following Council Directive 92/69 EEC, Method C.4-E

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3,4,5,6,7,8,9,10,11,12,13,14-dodecahydro-2H-cyclododeca[b]pyran

EC Number:

251-090-5

EC Name:

3,4,5,6,7,8,9,10,11,12,13,14-dodecahydro-2H-cyclododeca[b]pyran

Cas Number:

32539-83-6

Molecular formula:

C15H26O1

IUPAC Name:

2H,3H,4H,5H,6H,7H,8H,9H,10H,11H,12H,13H,14H-cyclododeca[b]pyran

Details on test material:

- Name of test material (as cited in study report): DDP
- Substance type: active
- Physical state: liquid
- Isomers composition: no isomers
- Purity test date: 1999-02-17
- Lot/batch No.: 9020015
- Expiration date of the lot/batch: 2000-07

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Secondary effluent of a domestic sewage treatment plant (Kläranlage Odenthal); TYPE: mixed population of aquatic microorganisms (activated sludge)
- Preparation of inoculum for exposure: separation of coarse particles by filtration
- Pretreatment: none
- Concentration of sludge: 5 ml/l
- Preconditioning: aeration for 6 days

Duration of test (contact time):

ca. 28 d

Initial test substance concentration

Initial conc.:

ca. 2.3 mg/L

Based on:

other: direct weighing

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: mixed population of aquatic microorganisms (activated sludge)
- Additional substrate: not used
- Solubilising agent (type and concentration if used): not used
- Test temperature: 20+-1°C
- pH: no data, but measured
- pH adjusted: no
- Aeration of dilution water: no data
- Suspended solids concentration: not possible as separation of coarse particles by filtration occurred
- Continuous darkness: yes
- Other: preconditioning of Inoculum: aeration for 6 days


TEST SYSTEM
- Culturing apparatus: not required
- Number of culture flasks/concentration: 10
- Method used to create aerobic conditions: no data
- Method used to create anaerobic conditions: not required
- Measuring equipment: SOP-No 2030-0000104-98-D Oxygen measurement instrument (Validity: 1998-03-05)


SAMPLING
- Sampling frequency: during 7 days, allows the assessment of percentage removal in a 14-day window
- Sampling method: measurement of the dissolved oxygen
- Sterility check if applicable: not required
- Sample storage before analysis: no data


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: not required
- Toxicity control: yes, 2.3 mg/l of test substance and 2.9 mg/l of reference substance
- Other:

STATISTICAL METHODS: no

Reference substance

Reference substance:

benzoic acid, sodium salt

Remarks:

99%, 2.9 mg/l

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

ca. 4

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

The reference substance, sodium benzoate, presented a degradation of 76% within 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The test material presented a biodegradation of 4%, and must be considered as not readily biodegradable under the test conditions.

Executive summary:

The biodegradation rate of test substance has been tested following the Biodegradability - Closed Bottle Test Method according to Council Directive 92/69/EEC, Method C.4-E. The test substances was suspended in a mineral medium, inoculated with a mixed population of aquatic microorganisms and incubated for 28 days under aerobic conditions in the dark at 20 ± 1°C. During this period, the biodegradation of the test substance was determined on the basis of the reduction of dissolved oxygen.

Within the test period of 28 days, a degradation of 4 % was determined for DDP. DDP has to be classified as "Not Readily Biodegradable" under the test conditions in accordance with the DSD (67/548/EEC) and CLP (1272/08/EEC).