Rhodium chloride (RhCl3), hydrate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

All test concentrations analysed at 0 and 96 hours

Vehicle:

not specified

Details on test solutions:

Test substance was applied in geometric dilution steps.

Test organisms (species):

Leuciscus idus melanotus

Details on test organisms:

Details on test organisms - source: Fischzucht Eggers Hohenwestedt
Details on test organisms - life stage of test species used: 4-7 cm at test start
Feeding: None during test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

20degress C

pH:

5.82 - 8.56

Dissolved oxygen:

Not measured due to the test substance causing the oxygen meter to fail, but test solutions aerated to maintain >60% oxygen saturation

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal concentrations: 1000, 800, 400, 200, and 100 mg/L.
Measured concentrations at 96 hours: 991, 837, 306, 85 and 52 mg/L
Measured concentrations at 96 hours used for biological evaluations

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass vessels containing 12L test solution
- Aeration: Yes:
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

OTHER TEST CONDITIONS
- Photoperiod: 16 h light:8 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality

TEST CONCENTRATIONS
- Range finding study: Yes
- Test concentrations: 10, 100 and 1000 mg/L
- Results used to determine the conditions for the definitive study: Yes

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

85 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

220 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

310 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Remarks:

yes - no mortality in the control, oxygen concentrations did not drop in the screening test, no oxygen measurements reported for the main test due to equipment failure. Results based on measured concentrations due to loss of the test substance.

Conclusions:

The 96 h LC50 of Rhodium (III) chloride hydrate to Leuciscus idus melanotus was 220 mg/L.

Executive summary:

Kemp (1995) is a GLP compliant study following OECD guideline 203. The loss of the test substance was taken into account by reporting results based on measured concentrations. pH was outside the desired limits, oxygen measurements could not be recorded during the main test, and there are some limitations with the reporting of the methods. However, despite the limitations this study is considered valid and suitable for use as a key study for this endpoint. The 96 h LC50 of Rhodium (III) chloride hydrate to Leuciscus idus melanotus was 220 mg/L.

Description of key information

The 96 h LC50 of Rhodium (III) chloride hydrate to Leuciscus idus melanotus was 220 mg test item/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

220 mg/L

Additional information

Kemp (1995) is a GLP compliant study following OECD guideline 203. The loss of the test substance was taken into account by reporting results based on measured concentrations. pH was outside the desired limits, oxygen measurements could not be recorded during the main test, and there are some limitations with the reporting of the methods. However, despite the limitations this study is considered valid and is assigned a Klimisch score of 2 (reliable with restrictions). The 96 h LC50 of Rhodium (III) chloride hydrate to Leuciscus idus melanotus was 220 mg/L.