2-tert-butylcyclohexyl ethyl carbonate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 13th January 2006 to 22nd March 2006

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with national standard methods

Remarks:

Performed as a preliminary study for a bioconcentration test so despite lack of details and analytical meassurement, the test method is highly standardised and the results considered acceptable for use as the test substance is known to be stable and the test solutions were at or lower than saturation concentration

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2006

Report date:

2006

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

No data

Deviations:

not specified

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-tert-butylcyclohexyl ethyl carbonate

EC Number:

267-184-4

EC Name:

2-tert-butylcyclohexyl ethyl carbonate

Cas Number:

67801-64-3

Molecular formula:

C13H24O3

IUPAC Name:

2-tert-butylcyclohexyl ethyl carbonate

Test material form:

liquid

Details on test material:

- Physical state: Colourless liquid
- Purity: see confidential data
- Lot/batch No.: see confidential data
- Expiration date of the lot/batch:see confidential data

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

not specified

Test solutions

Vehicle:

yes

Remarks:

Dimethyl sulfoxide and HCO-40

Details on test solutions:

Preparation of stock solution at 5000 mg/L with 2-tert-butylcyclohexyl ethyl carbonate diluted 10 x by volume in HCO-40

Test organisms

Test organisms (species):

Oryzias latipes

Study design

Test type:

semi-static

Water media type:

freshwater

Remarks:

Groundwater with a set of measured parameters including metal, residual chlorine, ammonia and pesticides, all below regulated levels

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

13.2 mg/L as Ca, Mg as measured in the ground water used in the study

Test temperature:

24.8-25.1°C

pH:

7.9-8.1

Dissolved oxygen:

6.4-8.1 mg/L

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

0, 1.25, 2.5, 5.0, 10.0 mg/L nominal
No measurements made but from other studies, if the initial concentrations are accurate then little test substance loss is expected during the study

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

3.86 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat. (dissolved fraction)

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

a 96h LC50 of 3.86 mg/L based on nominal concentrations was determined for Oryzias latipes.

Executive summary:

The study was performed as part of a preliminary test to determine the concentrations to use in a bioconcentration study using a standardised protocol including measurement of many of the required physico chemical parameters. Chemical analysis was not performed and LC50 values were based on nominal concentrations as the test substance was prepared in a stock solution containing vehicle and then diluted. All concentrations used in the study were below the solubility limit of the test substance (0, 1.25, 2.5, 5, and 10 mg/L ts). In the BCF study, analytical confirmation was performed and the dilutions were found to be very close to the nominal values and thus a good correlation between initial and nominal concentrations may also be expected in this study. Moreover, in the algae study, performed under static conditions in 2016, Kosak and Emnet found no significant loss of test substance over the 72 h study period so it is considered acceptable to base the LC50 on nominal concentrations for this substance.

In conclusion, a 96h LC50 of 3.86 mg/L based on nominal concentrations was determined for Oryzias latipes.