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EC number: 806-726-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2015-10-20 to 2015-10-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study according to GLP principles
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- The limit concentration and the control were analytically verified via LC-MS/MS analysis at the start (0 hours) and at the end of the exposure (48 hours). The method was validated prior to this study according to SANCO 3029/99 rev.4 (2000). At the start of the exposure (0 hours), sampling was carried out after preparation of the limit concentration. At the end of the exposure (48 hours), samples were taken directly from the test vessels.
- Vehicle:
- no
- Details on test solutions:
- The limit concentration (100 mg/L of the test item were weighed out) was freshly prepared with dilution water (ISO test water as specified below) before the start of the exposure (0 hours). The stock solution was mixed thoroughly by manual agitation.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna STRAUS is the preferred species in accordance with the test guidelines and is bred at the test facility. The daphnids were kept in glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium at 20 +/- 2°C in an incubator. The incubator is illuminated 16 hours per day with a light intensity of max. 20 µE/m2* s1. Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L, was used. The culture daphnids were fed at least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata and Desmodesmus subspicatus, with an algae cell density of > 106 cells/mL. The algae were cultured at the test facility.
20 daphnids, divided into 4 replicates, each with 5 daphnids, were used for the limit concentration and the control. At the start of the exposure 2 to 24 hours old daphnids from a healthy stock were used for the study. Juvenile daphnids were removed from the culture vessels latest 22 hours before the start of the exposure and discarded. The juveniles born within this period of max. 22 hours preceding the exposure were used for the test after additional 2 hours after collection. No first brood progeny was used for the test. Daphnids were left in dilution water for acclimatisation for at least 2 hours.
The daphnids were not fed during the study - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- nominal: 18 - 22°C, constant within ± 1°C
During the test period, the temperature in the incubator was 19 – 20 °C. - pH:
- Nominal test item start of exposure end of exposure
concentration (o hours) (48 hours, 4 Replicates)
100 mg/L 7.25 7.74 7.74 7.73 7.74
Control 7.85 7.68 7.74 7.79 7.82 - Dissolved oxygen:
- Dissolved O2 concentration [mg/L]
Nominal test item start of exposure end of exposure
concentration (o hours) (48 hours, 4 Replicates)
100 mg/L 8.71 8.37 8.46 8.62 8.57
Control 8.84 8.43 8.51 8.43 8.52 - Nominal and measured concentrations:
- The test was conducted as a limit test with anominal concentration of 100 mg/L. The measured concentration of the test item at the start of the exposure (0 hours) was 96.1 mg/L. At the end of the exposure (48 hours), the measured concentration of the test item was 110 mg/L. The measured test item concentrations were within ± 20 % of the nominal concentration. This indicates that the test item concentration was successfully maintained for the duration of the test. The effect levels are therefore based on the nominal concentration of the test item.
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- The effects of the limit concentration were deduced directly from the observed immobilisation rates. All effect levels (EC10 / 50 / 100) were based on the nominal concentration of the test item, because measured test item concentrations were within ± 20 % of the nominal concentration.
- Results with reference substance (positive control):
- The percentage immobility for the reference item was determined after 24 hours. The EC50-value with 95 % confidence limits (CI) was calculated by sigmoidal dose-response regression. The EC50-value for the most recent of the monthly performed reference tests was:
EC50: 1.61 mg/L (CI 1.43 - 1.82 mg/L)
The EC50-value of the reference item potassium dichromate after 24 hours is within the prescribed concentration range of 0.6 - 2.4 mg/L of quality criteria according to AQS P 9/2 (02/2000) for daphnids clone 5 cultured in Elendt M4 medium. The EC50-value of the reference item is also within the recommended range of 0.6 - 2.1 mg/L according to OECD-Guideline 202. - Validity criteria fulfilled:
- yes
- Conclusions:
- At the analytically confirmed nominal concentration of 100 mg/L of the test item, no effects were observed on Daphnia magna.
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS),the effects of a limit concentration of 100 mg/L of the test item were determined according to OECD 202 (2004). The limit test was conducted under static conditions over a period of 48 hours.
The limit concentration of 100 mg/L of the test item was freshly prepared with dilution water before the start of the exposure (at 0 hours) and mixed thoroughly by manual agitation. The limit concentration was visually clear throughout the exposure period.
Twenty daphnids each were exposed to the limit concentration and the control.
The concentrations of the test item were analytically verified via LC-MS/MS at the start (0 hours) and at the end of the exposure (48 hours) in the limit concentration and the control. The measured concentration of the test item at the start of the exposure (0 hours) was 96.1 mg/L and 110 mg/L at the end of the exposure (48 hours). The measured test item concentrations were within ±20 % of the nominal concentration. This indicates that the test item concentration was successfully maintained for the duration of the test therefore the effect levels are based on the nominal concentration of the test item.
The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 hours) and at the end of the exposure (48 hours), were within the acceptable limits. The validity criteria of the test guidelines were fulfilled.
Reference
Immobilisation Rates after 24 and 48 hours of Exposure in the Definitive Test (n = 20, divided into 4 replicates with 5 daphnids each)
Nominal test item concentration
[mg/L] |
IMMOBILISATION [%] |
|||||||||
24 hours |
48 hours |
|||||||||
Replicates |
Replicates |
|||||||||
1 |
2 |
3 |
4 |
MV |
1 |
2 |
3 |
4 |
MV |
|
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Measured Concentrations and Percent of the Nominal Concentration of the Test Item during the Definitive Test
Sampling date |
2015-10-20 Start of the exposure 0 hours |
2015-10-22 End of the exposure 48 hours |
||
Start of analysis |
2015-10-20 |
2015-10-22 |
||
Nominal test item concentration [mg/L] |
||||
Meas. conc. [mg/L] |
% |
Meas. conc. [mg/L] |
% |
|
100 |
96.1 |
96 |
110 |
110 |
Control |
< LOQ |
< LOQ |
Meas. conc. = measured concentration of the test item, mean value of 2 injections, dilution factors taken into account
% = percent of the nominal concentration of the test item
LOQ = limit of quantification (1 mg/L of the test item)Description of key information
In the acute immobilisation test with Daphnia magna (STRAUS),the effects of a limit concentration of 100 mg/L of the test item were determined according to OECD 202 (2004). The limit test was conducted under static conditions over a period of 48 hours.
The limit concentration of 100 mg/L of the test item was freshly prepared with dilution water before the start of the exposure (at 0 hours) and mixed thoroughly by manual agitation. The limit concentration was visually clear throughout the exposure period.
Twenty daphnids each were exposed to the limit concentration and the control.
The concentrations of the test item were analytically verified via LC-MS/MS at the start (0 hours) and at the end of the exposure (48 hours) in the limit concentration and the control. The measured concentration of the test item at the start of the exposure (0 hours) was 96.1 mg/L and 110 mg/L at the end of the exposure (48 hours). The measured test item concentrations were within ±20 % of the nominal concentration. This indicates that the test item concentration was successfully maintained for the duration of the test therefore the effect levels are based on the nominal concentration of the test item.
The water quality parameters (i.e. pH-value and dissolved oxygen concentration), measured at the start (0 hours) and at the end of the exposure (48 hours), were within the acceptable limits. The validity criteria of the test guidelines were fulfilled.
At the analytically confirmed nominal concentration of 100 mg/L of the test item, no effects were observed on Daphnia magna.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- > 100 mg/L
Additional information
At the analytically confirmed nominal concentration of 100 mg/L of the test item, no effects were observed on Daphnia magna.
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