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Diss Factsheets
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EC number: 942-376-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 Mar - 13 Apr 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- Version / remarks:
- March 2006
- Qualifier:
- according to guideline
- Guideline:
- ISO 14593:1999 (Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test))
- Version / remarks:
- 1999
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Staatliches Gewerbeaufsichtsamt, Hildesheim, Germany
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: aqueous phase of non adapted activated sludge, municipal sewage treatment plant, Hildesheim, Germany
- Pretreatment: sludge washed twice with autoclaved tap water; settled sludge was resuspended in mineral salts medium, aerated (4 h) and homogenized (blender); supernatant was decanted and maintained at aerobic conditions (aerated for 2 days with CO2-free air)
- Initial cell concentration: 1.3 x 10E+7 colony forming units (CFU)/L in the test vessel at test start - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- test mat.
- Initial conc.:
- 14.5 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 21 - 23 °C (down to 20 °C for 18 h)
- Continuous darkness: no, low light conditions
- Agitation: 150 - 200 rpm
TEST SYSTEM
- Number of culture flasks/concentration: triplicates (5 replicates at the last sampling)
- Measuring equipment: inorganic carbon measurement (according to DIN EN 1484) by a carbon analyser Multi N/C 3000, Analytic Jena
- Test performed in closed vessels: yes
- Details of trap for CO2 and volatile organics if used: sodium hydroxide solution used to convert CO2 to carbonate
SAMPLING
- Sampling frequency: on days 0, 2, 5, 7, 12, 14, 21, 28
- Sample storage before analysis: at 6 ± 2 °C if necessary
CONTROL AND BLANK SYSTEM
- Functional control: yes, triplicates (5 replicates at the last sampling)
- Inoculum control: yes, triplicates (5 replicates at the last sampling)
- Toxicity control: yes, triplicates (5 replicates at the last sampling) - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 79
- Sampling time:
- 28 d
- Details on results:
- 10% of the biodegradation occured after 4 days, 60% was reached after 12 days and 79% of the test item was biodegraded after 28 days. The 95% confidence interval on day 28 was 74 - 84%.
- Results with reference substance:
- Transition of adaptation to degradation phase occured after 1 day (>=10% biodegradation). 60% of the substance was biodegraded after 3 days, which fulfills the validity criterion of 60% degradation after 14 days. 92% of the substance was biodegrated after 28 days. The 95% confidence interval on day 28 was 91 - 93%.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The substance was found to be readily biodegradable under the test conditions, with a mean percentage biodegradation of 79% at the end of the 28-day exposure period. The pass level for ready biodegradability was reached on exposure day 12.
Reference
The biodegradation of the toxicity control reached 73% within 14 days and it came to 84% after 28 days. The biodegradation of the reference item was not inhibited by the test item in the toxicity control. The 95% confidence interval on day 28 was 83 – 85%.
Table 1: Biodegradation in the test item, toxicity control and functional control samples in % as well as the corresponding 95% confidential interval on day 28
|
Degradation [%] |
Confidence Interval on Day 28 |
|||||||
Day |
0 |
2 |
5 |
7 |
12 |
14 |
21 |
28 |
P=95% |
Test Item, 20 mg/L |
- |
0 |
20 |
49 |
62 |
65 |
72 |
79 |
74 – 84 |
Functional Control, 30 mg/L |
- |
54 |
76 |
80 |
86 |
90 |
87 |
92 |
91 – 93 |
Toxicity Control, 20mg/L Test Item + 30 mg/L Reference Item |
- |
- |
- |
58 |
- |
73 |
78 |
84 |
83 - 85 |
Description of key information
Readily biodegradable (79% within 28 d, OECD 310)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
One GLP guideline study according to OECD 310 is available investigating the ready biodegradability of the substance. A mixed microbial population of activated sludge of a local municipal STP was inoculated with a nominal concentration of 20.0 mg/L test substance (corresponding to a carbon content of 14.5 mg C/L) and incubated for 28 d under controlled conditions at low light conditions. Degradation was followed by the determination of dissolved oxygen content after 0 h, 7, 14, 21 and 28 d. An inoculum blank, a functional control (with 30 mg reference substance/L) and a toxicity control (20 mg/L test item and 30 mg/L Reference item) were run in parallel. The 10% level (beginning of biodegradation) was reached after 4 days. The pass level of 60% was reached after 12 days and the biodegradation came to 79% after 28 days. The percentage degradation of the functional control reached the pass level of 60% after 3 days and came to 90% after 28 days. In the toxicity control containing both test and reference item a biodegradation rate of 73% occurred within 14 days and it came to 84% after 28 days. Based on these facts the test item is classified as readily biodegradable. All validity criteria of the study were met.
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