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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-08-10 to 2019-04-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Details on preparation and application of test substrate:
- 2.5033 g test item was weighed and mixed with 50g industrial sand. The treated sand was mixed thoroughly with proper artificial soil (dry weight was 2450 g). 250 ml distilled water was added to adjust the moisture content to about 30% of dry weight and mixed to be homogeneous. The complete mixture was moist but not so wet that water appears when the artificial soil was compressed. For each treated soil, four 750 g of the wet test medium were weighed and put into four labelled test containers.
A concurrent control group with four replicates maintained under identical conditions only without test item application was performed in parallel.
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- The test earthworm (Eisenia foetida) was obtained from Chenggong Earthworm Raising Farm in Dongli District, Tianjin. The domestication conditions of earthworms were 20±2°C, 80±15% relative humidity and continuous light.
The adult (at least two months old with clitellum) worms with an individual weight of 0.3008~0.4352 g were used in the test.
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- During the test, monitored temperature was in the range of 19.3~20.6 °C.
- pH:
- At the beginning of the test, pH value was 5.51 and 5.13 for control and test medium, respectively.
- Moisture:
- 66~89 % relative humidity
- Details on test conditions:
- Four replicateswere used for the test group and control group. For each test container, 10 earthworms (which had been conditioned in an artificial soil, washed, dried on filter paper and weighed before use) were placed on the surface of test medium. The containers were covered with a glass plate to prevent the earthworms from escaping and the test medium from drying. The containers were kept under the test conditions for 14 days.
The mortality was assessed by emptying test medium onto a plate, sorting worms from the medium and testing their reaction to a mechanical stimulus at the front end at the 7th and 14th day. Any behavioural signs was recorded. After the 7-day assessment worms and medium was replaced in the test containers.
At the start and the end of the test, the individual weights of all live worms in each group were measured and recorded. The worms were washed prior to weighing (with distilled water) and the excess water was removed by placing the worms briefly on filter paper. - Nominal and measured concentrations:
- 1000 mg/kg dry soil weight
Results and discussion
Effect concentrations
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- During the exposure period, there was no mortality or abnormal signs observed at control and 1000 mg/kg dry soil weight test group. According to the results, the acute toxicity of Sa 163 was:
LC50(14 d): > 1000mg/kg dry soil weight. - Executive summary:
Based on the results of the preliminary test, the definitive study was conducted with a limit concentration of 1000 mg/kg dry soil weight. A concurrent control group was conducted in parallel. For the test and control group, 4 replicates were used with 10 worms in each replicate. Artificial soil was used in this study. The test duration was 14 days, assessment of mortality and abnormal signs was conducted at 7 and 14 day of test.
During the exposure period, there was no mortality or abnormal signs observed at control and 1000 mg/kg dry soil weight test group.According to the results, the acute toxicity of Sa 163 was:
LC50(14 d): > 1000mg/kg dry soil weight.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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