Tetrabutylammonium iodide

Endpoint summary

Description of key information

Short-term toxicity to fish

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for (test item name) Tetrabutylammonium iodide The nominal concentration selected for the experiment were 100mg/Land test fish were exposed to these concentration for 96 hours.

The lethal concentrations LC50 was found to be >100 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = No mortality was observed

LC50 (96 hours) Experimental = >100 mg/L

Short term toxicity to aquatic invertebrates:

Short term toxicity to aquatic invertebrates was performed according to the OECD guideline 202. The test was performed in 5 neonates having age of <24 hr in static fresh water condition for 20 degree C under 6 different concentrations. 100 ml beaker with 40 ml of test solution was taken for experiment. The temperature was 20 1 1C, with a 16/8 h light/dark photoperiod. Each test vessel was checked for immobilized individuals at 24 and 48 h after the beginning of the test.After the experiment, the EC 50 value for N, N, N-tributylbutan-1-aminium iodide (311-28-4) was determined to be 2.8 mg/l for 48 hrs. Based on value , N, N, N-tributylbutan-1-aminium iodide (311-28-4) was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 2 category.

Toxicity to aquatic algae and cyanobacteria

The short-term toxicity of the test substance N,N,N-tributylbutan-1-aminium iodide (CAS no. 311 -28 -4) to green algae is predicted using EPI Suite ECOSAR version 1.10 (EPI Suite, 2017). On the basis of effects observed in a static freshwater system during a 96 hr exposure, the effect concentration (EC50) for the substance is estimated to be 203.268 mg/L. Based on this value, it can be concluded that the test chemical N,N,N-tributylbutan-1-aminium iodide can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered to be not classified as per the CLP classification criteria.

Toxicity to microorganisms

Vibrio fischeri in a 15 min inhibition of bioluminescence test the 50% effect concentrations (EC50) of the ammonium salts TBAI was observed to be 284 mg/l with 95% confidence limit (241–336)..

Additional information

Short-term toxicity to fish

Two studies including experimental and predicted data for short term fish toxicity of target chemical 1-Butanaminium, N,N,N-tributyl-, iodide (1:1) (Cas no.311-28-4) were summarised as follows:

Key study from UERL laboratory result;2017 suggest the Fish Acute Toxicity test according to OECD Guideline 203 was conducted for (test item name) Tetrabutylammonium iodide The nominal concentration selected for the experiment were 100mg/Land test fish were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be >100 mg/L and LC0 (96 hours) (highest loading at which no mortality was observed) = No mortality was observed whereas LC50 (96 hours) Experimental = >100 mg/L.

And supporting report from EPI Suite ECOSAR version 1.10 predicted model, the 96 hours LC50 was estimated to be 551.025 mg/l on Fish for substance N,N,N-tributylbutan-1-aminium iodide (CAS no.311-28-4) on the basis of mortality effects using neutral organics class for prediction.Based on this value, it can be concluded that the test chemical N, N, N-tributylbutan-1-aminium iodide (CAS no 311-28-4) can be considered as non-toxic to fish at environmentally relevant concentrations and can be considered to be not classified as per the CLP regulations.

Thus based on the both results for short term fish concluded that the test substance 1-Butanaminium, N,N,N-tributyl-, iodide (1:1) (Cas no.311-28-4) was non toxic to fish for acute exposure thus not consider for the aquatic classification as per the CLP criteria.

Short term toxicity to aquatic invetebrates:

Short term toxicity to aquatic invertebrates was summaries with one experimental data and one prediction report for target is as follows:

 

Study was performed on N,N,N-tributylbutan-1-aminium iodide by Paola Gallettiet,al, New Journal of Chemistry,2009, 33, 1859–1868, 11th February 2009, according to the OECD guideline 202. The test was performed in 5 neonates having age of <24 hr in static fresh water condition for 20 degree C under 6 different concentrations. 100 ml beaker with 40 ml of test solution was taken for experiment. The temperature was 20 1 1C, with a 16/8 h light/dark photoperiod. Each test vessel was checked for immobilized individuals at 24 and 48 h after the beginning of the test. After the experiment, the EC 50 value for N, N, N-tributylbutan-1-aminium iodide (311-28-4) was determined to be 2.8 mg/l for 48 hrs. Based on value, N, N, N-tributylbutan-1-aminium iodide (311-28-4) was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 2 category as per the CLP regulation.

 

Prediction was done using the OECD QSAR toolbox version 3.4 with log kow as the primary descriptor and considering the five closest read across substances, the short term toxicity on aquatic invertebrates was predicted for N, N, N-tributylbutan-1-aminium iodide (CAS:311-28-4). EC50 value was estimated to be 56.57 mg/L on the basis of intoxication for Daphnia magna for 48 hrs duration. Based on value, N, N, N-tributylbutan-1-aminium iodide (311-28-4) was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 3 category as per the CLP regulation.

 

Based on the prediction data for N,N,N-tributylbutan-1-aminium iodide, it was classified in aquatic chronic 3 category but as experimental data suggested aquatic chronic 2 category classification for N,N,N-tributylbutan-1-aminium iodide, so it can be concluded that N, N, N-tributylbutan-1-aminium iodide (311-28-4) was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 2 category as per the CLP regulation.

 

Toxicity to aquatic algae and cyanobacteria

1 predicted data for the target chemical N,N,N-tributylbutan-1-aminium iodide(CAS No. 311-28-4) which are supported further by the total 2 weight of evidence studies (1 study from peer reviewed journal and 1 study is from secondary source) for its closest read across substance with logKow as the primary descriptor were reviewed for the toxicity to aquatic algae and cyanobacteria end point which are summarized as below:

 

The short-term toxicity of the test substance N,N,N-tributylbutan-1-aminium iodide (CAS no. 311 -28 -4) to green algae is predicted using EPI Suite ECOSAR version 1.10 (EPI Suite, 2017). On the basis of effects observed in a static freshwater system during a 96 hr exposure, the effect concentration (EC50) for the substance is estimated to be 203.268 mg/L. Based on this value, it can be concluded that the test chemical N,N,N-tributylbutan-1-aminium iodide can be considered as non-toxic to green algae at environmentally relevant concentrations and can be considered to be not classified as per the CLP classification criteria.

 

In a weight of evidence study of read across compound Tetrabutylammonium bromide (CAS no. 1643-19-2), freshwater algal growth inhibition test was carried out for 96 hr on Pseudokirchneriella subcapitata with the substance Tetrabutylammonium bromide (CAS no. 1643-19-2) according to OECD Guideline 201 (Alga, Growth Inhibition Test) and EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II), respectively (Chul-Woong Cho et. al; 2008). The study was based on the effects of the read across compound Tetrabutylammonium bromide on Pseudokirchneriella subcapitata in a flowthrough fresh watersystem. The green microalga used as the model algal strain in this study, Selenastrum capricornutum ATCC-22662, was obtained from the National Institute Environmental Research, Korea. The stock alga was cultivated in 250 ml Erlenmeyer flasks, containing 200 ml sterilized nitrate-enriched BBM medium prepared in triple distilled water, to avoid nitrogen limitation during the high-density culture. Experiments were performed in 250 ml Erlenmeyer flasks, containing 55ml of sterilized culture medium, inoculated with 5ml samples of 7-day cultured algae. Solutionsof ionic liquids and organic solvents were subsequently added to the test flasks. The flasks then were placed on a shaker incubator at 170 rpm and 25 deg C, with 24 h light supplied via warm-white fluorescent tubes, with an average illumination of 3075 mEm2 s1. At each determined exposure date, the optical density of the algal biomass was estimated at 438nm using a spectrophotometer (UV mini-1240, Shimadzu, Kyoto, Japan) and average specific growth rate determined. The dry cell weight, corresponding to theoptical density, was determined from the linear relationship; dry cell weight (g/l) = 0.1329 optical density.

Based upon the effect on the growth rate of the test organism Pseudokirchneriella subcapitata, the 48, 72 and 96hr EC50 value was determined to be 300.84, 1542.94 and 721.689 mg/L, respectively. Thus, based on this value, it can be concluded that the read across chemical Tetrabutylammonium bromide can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

 

Another weight of evidence study of short term toxicity to Selenastrum capricornutum (green algae) was carried out for 72 hrs of read across compound N-(carboxymethyl)-3 -(dodecanoylamino)-N,N-dimethylpropan-1 -aminium hydroxide (CAS no. 4292-10-8). The study was performed according to EU Method C.3 (Algal Inhibition test) (High Production Volume Challenge Program; 2016). The study was based on the effects of the read across compound N-(carboxymethyl)-3 -(dodecanoylamino)-N,N-dimethylpropan-1 -aminium hydroxide (CAS no. 4292-10-8) on Selenastrum capricornutum (green algae) in a static fresh water system. Selenastrum capricornutum was used as a test organism. Algae were exposed to the test material at a concentration of 100 mg/l for 72 hours. The flasks containing the control and the treated organisms were stirred at a temperature of 23 ± 2 °C with continuous lighting for 72 hours. After 24, 48 and 72 hours, cell concentration was measured with a Burke chamber. The pH of the culture medium was 8.0 ± 0.5. After 24, 48 and 72 hours, cell concentration was measured with a Burke chamber. After the exposure period of 72 hrs, no difference in algal growth was seen between treated and control groups. The difference between the biomass of the control and treated groups is less than 25%. The difference between the average growth of the treated group and control group was 0.56%.The temperature during the test did not vary more than 1 °C and the pH did not vary more than 0.2 units. Based on effect on growth rate and biomass of the test organism Selenastrum capricornutum (green algae), the 72 hr NOEC and EC50 value was determined to be 100 and > 100 mg/l. Thus, based on the EC50 value, it can be concluded that the read across substance N-(carboxymethyl)-3 -(dodecanoylamino)-N,N-dimethylpropan-1 -aminium hydroxide can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP classification criteria.

 

Based on the overall reported results for target chemical N,N,N-tributylbutan-1-aminium iodide (from EPI Suite, 2017) and for its read across substance (from peer reviewed journal and secondary source), it can be concluded that the test substance N,N,N-tributylbutan-1 -aminium iodide can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP classification criteria.

Toxicity to microorganisms

Toxicity to V. fischeri was measured as inhibition of bioluminescence using Microtoxs M500 Rapid Toxicity Testing System equipment and consumables.The assay was carried out in accordance with the 90% basic test for pure compounds protocol, as described in the Microtox user’s manual.

The EC50 values of the tested ammonium salts for V. fischeri range from 1.3 to 284 mg L1. The differences tested by ANOVA are highly significant (P o 0.001).

In experiment the 50% effect concentrations (EC50) of the ammonium salts TBAI to Vibrio fischeri in a 15 min inhibition of bioluminescence test was observed to be 284 mg/l with 95% confidence limit (241–336).Based on the available effective concentration (EC50) suggest that the test substance N,N,N-tributylbutan-1-aminium iodide (Cas no. 311-28-4) non toxic to micro organism for acute exposure period.