Enzymatic hydrolysis products of Ophiopogon japonicus, Liliaceae, root

Density

Link to relevant study record(s)

Reference

Endpoint:

density

Remarks:

density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 18 to 26 April 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

The study was conducted according to OECD/EU guideline, and under GLP. No deviation affected the quality of the study. The test substance is adequately identified. Therefore full validation applies.

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Deviations:

no

GLP compliance:

yes

Type of method:

pycnometer method

Key result

Type:

density

Density:

ca. 1.275 g/cm³

Temp.:

20 °C

Remarks on result:

other: 1.2748 ± 0.0119 g/cm3 at 20.0 ± 0.4 °C, which is the mean of two values ± the standard deviation

Results

Results of the single determinations with the respective error as well as the mean value and its standard deviation are presented in the following tables:

Table 4.4/1    Results of Single Determinations

Determination	Density in g/cm^3	Error of Determ. in g/cm^3
1	1.2831	0.0003
2	1.2664	0.0003

 

 

Table 4.4/2     Results – Mean Values

Parameter	Value	Unit	Standard Deviation	Relative Standard Deviation
Volume of Pycnometer	50.38	cm^3	0.0062	0.01%
Density of Test Item	1.2748	g/cm^3	0.0119	0.93%
Density of Test Item	1274.8	kg/m^3	11.9	0.93%
Relative Density of Test Item (d^204)	1.2748		0.0119	0.93%

 

Discussion

The density of the test item was calculated as 1.2748 ± 0.0119 g/cm³at 20.0 ± 0.4°C,which is the mean of two values± the standard deviation.Standard deviation was 0.0119 g/cm³, showing good correlation of the two determinations.

Therefore, the study is considered valid.

Conclusions:

The test substance is less dense than water. The density of the test item was calculated as 973.2 kg/m3 at 23.0 ± 0.2°C, which is the mean of two values.
Standard deviation was 5.2 kg/m3, showing good correlation of the two determinations.

Executive summary:

The relative density of the test substance was measured according OECD 109, by pycnometer method.Two runs were performed, and the mean density at 23.0 °C was calculated.

The density of the test item has been determined to be 973.2 kg/m3 at 23.0 ± 0.2°C.

Standard deviation was 5.2 kg/m3, showing good correlation of the two determinations.

Description of key information

The density of the test item has been determined to be 1.2748 ± 0.0119 g/cm3 at 20.0 ± 0.4 °C, with a corresponding relative density value of 1.2748

Key value for chemical safety assessment

Relative density at 20C:

1.275

Additional information

A fully reliable experimental study, conducted according to a recognized OECD/EC method (pycnometer method) and under GLP, is available. It is considered as a key study.

Two runs were performed, and the mean density at 20.0 °C was calculated. This result was then converted to relative density towards water at 4 °C.

The result is retained as key data.