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EC number: 432-070-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 1997- December 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- OECD 202
- Deviations:
- not specified
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: no melting point/range (Decomposition at temperatures > 200 °C)
- Boiling point: no boiling point (decomposition before boiling)
- Vapour pressure: <1 * 10-12 hPa at 25°C (calculation)
- Water solubility: 125.76 g/L at 20°C
- log Pow: <-2
- pKa: = 7,00+/-0,04 - Analytical monitoring:
- no
- Details on sampling:
- analysis of the concentrations were not been performed.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- method:the test article is a well soluble powder with strongly reducing properties. This would lead to total oxygen depletion in the test solutions. In arrangement with Dr. Peter (German environmental bureau, UBA, telephone call with Dr. Kuttler on 27 th October 1997) the test article was solved in the culturing medium (c= 10 g/l) and aerated with cleaned compressed air until complete oxidation. The time required for the oxidation amounted to 24 h at 20°C. Beginning at a redox potential of - 204 mV aeration changed the potential to + 85 mV after 24 h. The pH-value was regulated from 9,96 (after oxidation) to 7.8 +/- 0.2. The test solutions were produced by diluting this stock solution. - Test organisms (species):
- other aquatic crustacea: Daphnia Magna, Clone 5
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna, Crustacea, Phyllopoda, Cladocera, Daphniidae
- Strain:clone 5
- Source: German Environmental Ministry
- Feeding during test: none
- water: see " details on test conditions "
Pre-treatment:
The holding of the animals is carried out in a non climated romm (22°C +/- 5°C, light/dark = 14h/10h; illumination: Grolux Aqua star) in 1L glass beakers. The animal are fed 3 times a day with alga (scenedesmus subspicatus) and once a day with washed activated sludge. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- no data available
- Hardness:
- total hardness: 2.5 mmol/L
- Test temperature:
- 20°C +/- 2°C (Incubator, data given in the study report under "test conditions")
19.3 - 20.2 °C (data given in the study report under "results") - pH:
- 7.4 - 8.1
- Dissolved oxygen:
- > 65% over the test period in all vessels
- Salinity:
- stock solutions in demineralized water:
- synthetic sea salt (Wimex hw Meersalz, containing 7 macro nutrients, 60 trace elements, 8 vitamins and 3 amino acids): 0.333 g/L medium
- CaCl2 x 2H2O (117.6 g/L): 2.3 mL/Lmedium
- NaHCO3 (25.2 /L): 2.2 mL/L medium
- SeO2 (1.4 g/L): 0.1 mL/L medium - Nominal and measured concentrations:
- concentrations: 10.000 mg/L, 5.000 mg/L, 2500 mg/L, 1250 mg/L, 625 mg/L, Control
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 ml glass beakers
- Aeration: none
- No. of organisms per concentration: 20 animals/conc (4 x 5 in 20 mL, 50 mL glass beakers)
-Duration: 48 h
TEST MEDIUM / WATER PARAMETERS
Source/preparation of dilution water: the culturing medium (stock solutions in demineralized water):
-synthetic sea salt (Wimex hw Meersalz, containing 7 macro nutrients, 60 trace elements, 8 vitamins and 3 amino acids): 0.333 g/L medium
- CaCl2 x 2H2O (117.6 g/L): 2.3 mL/Lmedium
- NaHCO3 (25.2 /L): 2.2 mL/L medium
- SeO2 (1.4 g/L): 0.1 mL/L medium
This water is aerated with oil free compressed air until saturation with oxygen and the pH is adjusted to 7.8 +/- 0.2.
- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1
OTHER TEST CONDITIONS
- Adjustment of pH: the pH is adjusted to 7.8 +/- 0.2.
- Illumination: 14h light, 10 h dark (Gro-Lux)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
pH-value, dissolved oxygen value and temperature are measured at the beginning and at the end of the test.
TEST CONCENTRATIONS
- Test concentrations: 10000, 5000, 2500, 1250, 625 mg/L - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4 400 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 2 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: The surviving animals in 5 and 10 g/L are very weak by the end of the test, while the vitality of the animals in all other concentrations (till 2.5 g/L) resemble the control animals.
See also table 1. - Results with reference substance (positive control):
- no reference substance used in the study
- Reported statistics and error estimates:
- none
- Validity criteria fulfilled:
- yes
- Conclusions:
- The test item is not acute toxic for daphnia.
- Executive summary:
Observations: The surviving animals in 5 and 10 g/L are very weak by the end of the test, while the vitality of the animals in all other concentrations(till 2,5 g/L} resemble to the control animals.
EC50(24h): 9500 mg/L
EC50(48h): 4400 mg/L
Evaluation: The test item is not acute toxic for daphnia.
Reference
table 1: Immobilisation rate, skinnings an no. of animals which are trapped at the surface after 48 h
Control | 625 [mg/L] | 1250 [mg/L] | 2500 [mg/L] | 5000 [mg/L] | 10000 [mg/L] | |
Immobilisation [%], 24 h | 0 | 0 | 0 | 0 | 0 | 60 |
Immobilisation [%], 48 h | 0 | 0 | 0 | 0 | 85 | 90 |
Skinnings [%], 48 h | 100 | 100 | 100 | 100 | 75 | 50 |
animals trapped at thesurface [%], 48 h | 0 | 0 | 0 | 0 | 5 | 0 |
Description of key information
Not acute toxic to aquatic invertebrates (daphnia).
EC50(24h): 9500 mg/L
EC50(48h): 4400 mg/L
Evaluation: The test item is not acute toxic for daphnia.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 9 500 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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