2-Pentanone, O,O',O''-(ethenylsilylidyne)trioxime

Hydrolysis

Administrative data

Endpoint:

hydrolysis

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

15 March 2012-19 June 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The test procedure was well described in the study report, based on the EC, OECD and OPPTS hydrolysis test guidelines and performed according to GLP principles.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)

Version / remarks:

(2008)

Deviations:

yes

Remarks:

a test based on the guideline was performed

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 111 (Hydrolysis as a Function of pH)

Version / remarks:

(2004)

Deviations:

yes

Remarks:

a test based on the guideline was performed

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 835.2120 (Hydrolysis of Parent and Degradates as a Function of pH at 25°C)

Version / remarks:

(2008)

Deviations:

yes

Remarks:

a test based on the guideline was performed

Principles of method if other than guideline:

Quick hydrolysis of the substance was expected. To confirm this, the following test was performed instead of the tests outlined in the test guidelines.

5 ml of the test substance was mixed with 10 and 25 ml of each buffer (pH 4, 7 and 9) in a glass vessel. Each vessel was inverted 10 times by hand and the phases were allowed to separate. 4 minutes and 1 hour after preparation, a sample was taken from the upper organic phase and analysed. Chromatograms of the pretreated samples were compared with a chromatogram of untreated test substance. The test was performed at room temperature.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Test material form:

other: Colourless to mild yellowish liquid

Details on test material:

- Name of test material (as cited in study report): OS 2600
- CAS no.: 58190-62-8

During performance of the test, the test substance and test substance containing solutions were protected from light as much as possible.

Radiolabelling:

no

Study design

Analytical monitoring:

no

Details on sampling:

All test samples were analysed by single injection.

Buffers:

Acetate buffer pH 4, 0.1 M: solution of 16.7% (v/v) 0.1 M sodium acetate and 83.3% (v/v) 0.1 M acetic acid. The buffer contains 0.0009% (w/v) sodium azide.

Phosphate buffer pH 7, 0.1 M: solution of 0.1 M potassium dihydrogenphosphate adjusted to pH 7 using 10 N sodium hydroxide. The buffer contains 0.0009% (w/v) sodium azide.

Borate buffer pH 9, 0.1 M: solution of 0.1 M boric acid and 0.1 M potassium chloride adjusted to pH 9 using 10 N sodium hydroxide. The buffer contains 0.0009% (w/v) sodium azide.

Results and discussion

Preliminary study:

Several small and large peaks were observed in the chromatogram of the test substance solution. It was assumed that the large peaks derive from the major components of the test substance, whereas the small peaks derive from impurities. The peak area of the major components with a retention time between 5.5 and 6.5 minutes was used as response in the calculations.

Any other information on results incl. tables

Table  Test substance response at pH 4, pH 7 and pH 9

 

Date of analysis [dd-mm-yy]	Volume buffer solution [ml]	pH code	Sampling time [minutes]	response [area]	Relative concentration [%]
15-03-12	10	pH 4	n.a.	5483201
			4	31017	0.57
			60	75758	1.4
21-03-12	10	pH 7	n.a.	18732062^1
			4	2522	0.01
			60	0	0.00
21-03-12	10	pH 9	n.a.	18732062^1
			4	7335141	39
			60	11049995	59
15-03-12	25	pH 4	n.a.	5483201
			4	0	0.00
			60	1152	0.02
21-03-12	25	pH 7	n.a.	18732062^1
			4	0	0.00
			60	0	0.00
21-03-12	25	pH 9	n.a.	18732062^1
			4	266090	1.4
			60	102510	0.55

n.a.  not applicable; response of untreated test substance

1  average of two measurements; individual peak areas were 17681703 and 19782420

 

Applicant's summary and conclusion

Conclusions:

A screening test based on EC C.7, OECD 111, EPA OPPTS 830.2120 guidelines and performed under GLP, showed that the substance hydrolysis very fast at room temperature and pH 4, 7 and 9 (half-life time < 4 minutes).