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EC number: 612-182-4 | CAS number: 6161-65-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2001
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No guideline study. Non-GLP. Test system only appropriate to address skin corrosion, but not irritaion effects.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Guideline:
- other: EpiDerm (TM) Corrosivity-Test in vitro (OECD 431)
- Principles of method if other than guideline:
- Basic procedure of the EpiDerm (TM) Corrosion test:
From the day of arrival in the laboratory, tissues were kept in the refrigerator. At least 1 hour but not more than 1.5 hours before test-substance application, tissues were transferred to 6-well plates with 0.9 mL assay medium and preconditioned in the incubator at 37°C. The preincubation medium was replaced with fresh medium immediately before application. Two tissues per exposure time (3 minutes at room temperature or 1 hour in the incubator, as a rule) and test group (test material, negative control and positive control; 12 tissues per test) were used. 25 μL highly de-ionized water was applied first. Thereafter, a bulk volume of 25 μL of the test material was applied with a sharp spoon and homogeneously distributed with the water. Control tissues were concurrently applied with 50 μL of highly de-ionized water (negative control, NC) or with 50 μL of 8 n potassium hydroxide (positive control, PC). A nylon mesh was placed carefully onto the tissue surface of the NC afterwards. The tissues were washed with PBS to remove residual test material 3 minutes or 1 hour after start of the application treatment. Rinsed tissues were kept in 24-well plates (holding plates) at room temperature on assay medium until all tissues per application time were dosed and rinsed. The assay medium was then replaced by MTT solution and tissues were incubated for 3 hours. After incubation, tissues were washed with PBS and the formazan produced by the tissues was extracted with isopropanol over night at room temperature. The optical density at a wavelength of 570 nm (OD570) of the extracts was determined spectrophotometrically. Blank values were established of 6 microtiter wells filled with isopropanol for each microtiter plate. - GLP compliance:
- no
Test material
- Reference substance name:
- 2-Methoxy-6-methylbenzoic acid
- EC Number:
- 612-182-4
- Cas Number:
- 6161-65-5
- Molecular formula:
- C9H10O3
- IUPAC Name:
- 2-Methoxy-6-methylbenzoic acid
- Details on test material:
- - batch number: 30529/6
- substance number: 01/0246-1
- CAS number: 6161-65-5
- test substance name: BAS 560 F - (Benzoe-)Säure
Constituent 1
Test animals
- Species:
- other: in vitro
Results and discussion
Any other information on results incl. tables
No direct MTT reduction observed.
Exposure: 3 min | ||||||
Test article | OD 570 tissue 1 | OD 570 tissue 2 | OD 570 KC | mean OD 570 | mean OD 570 KC corrected | viability [% of NC] |
NC | 2.008 | 1.997 | – | 2.002 | – | 100 |
01/0246-1 | 1.988 | 2.041 | – | 2.014 | – | 101 |
PC_C | 0.323 | 0.303 | – | 0.313 | – | 16 |
Exposure: 1 h | ||||||
Test article | OD 570 tissue 1 | OD 570 tissue 2 | OD 570 KC | mean OD 570 | mean OD 570 KC corrected | viability [% of NC] |
NC | 1.887 | 1.955 | – | 1.921 | – | 100 |
01/0246-1 | 1.823 | 1.936 | – | 1.879 | – | 98 |
PC_C | 0.113 | 0.17 | – | 0.141 | – | 7 |
NC = negative control | ||||||
PC_C = positive control | ||||||
OD 570 = optical density [wavelength 570 nm] | ||||||
KC = killed tissue control (only included if applicable) |
Applicant's summary and conclusion
- Interpretation of results:
- other: not corrosive
- Conclusions:
- Based on the results of this study, a classification of the test substance for skin corrosion is not warranted. However, the design of this experiment does not allow the evaluation of skin irritation effekts.
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