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EC number: 213-138-3 | CAS number: 926-57-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Adopted according to OECD SIDS (public available peer reviewed source). The original source is not available and has not been reviewed.
Data source
Referenceopen allclose all
- Reference Type:
- secondary source
- Title:
- 1,3-Dichlorobut-2-ene - CAS No: 926-57-8
- Author:
- OECD SIDS
- Year:
- 2 007
- Bibliographic source:
- SIDS Initial Assessment Report for 22th SIAM, UNEP Publications
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OTS 798.5395 (In Vivo Mammalian Cytogenics Tests: Erythrocyte Micronucleus Assay)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 1,3-dichlorobut-2-ene
- EC Number:
- 213-138-3
- EC Name:
- 1,3-dichlorobut-2-ene
- Cas Number:
- 926-57-8
- Molecular formula:
- C4H6Cl2
- IUPAC Name:
- 1,3-dichlorobut-2-ene
- Details on test material:
- - Name of test material (as cited in study report): 1,3-dichlorobut-2-ene
- Analytical purity: 98%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Crl:CD(R) BR
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 47 days old
- Housing: rats were housed in suspended, stainless steel wire-mesh cages.
Administration / exposure
- Route of administration:
- inhalation
- Vehicle:
- Conditioned, filtered, house-line air was the negative (vehicle) control.
- Details on exposure:
- TYPE OF INHALATION EXPOSURE: nose only
GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
Test atmospheres were generated by metering nitrogen over or through glass impingers containing a reservoir holding 1,3-dichlorobut-2-ene. The nitrogen swept the 1,3-dichlorobut-2-ene vapours into each test chamber via Teflon tubing. The concentration in each chamber was controlled by varying the flow of nitrogen to the impinger. The impingers were kept in a 15ºC bath and were isolated from the rest of the generation system. Filtered, dried dilution air was introduced into each chamber at 40 L/min. For the control chamber, the air flow was 28 L/min.
During exposure, each rat was individually confined in a polycarbonate restrainers placed in a glass and stainless steel 150-L chamber. Only the nose of the animal protruded into the chamber. The positions of the animals within the chambers were randomly rotated daily. The chambers were operated in a one-pass, flow-through mode with air flow rates adequate to provide 16 air changes/hour for the air control chambers. The flow rates provided sufficient oxygen for the test animals and adequate distribution of the test material in the chambers.
At approximately 30-minute intervals, chamber samples of the test atmospheres were drawn and delivered to a gas chromatograph. During exposures, the relative humidity, temperature, and oxygen concentration of each chamber were recorded. An Omega thermocouple was used to measure chamber temperatures, a psychrometer was used to measure relative humidity, and an oxygen monitor was used to measure chamber oxygen.
TEST ATMOSPHERE
At approximately 30-minute intervals, chamber samples of the test atmospheres were drawn and delivered to a gas chromatograph. During exposures, the relative humidity, temperature, and oxygen concentration of each chamber were recorded. An Omega thermocouple was used to measure chamber temperatures, a psychrometer was used to measure relative humidity, and an oxygen monitor was used to measure chamber oxygen. - Duration of treatment / exposure:
- 2 weeks
- Frequency of treatment:
- 6 hours/day, 5 days/week
- Post exposure period:
- no data
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 10, 50, 100 ppm
Basis:
nominal conc.
- No. of animals per sex per dose:
- 5
- Control animals:
- yes
- yes, concurrent vehicle
- Positive control(s):
- The positive control indicator was cyclophosphamide (CP). The rats in the positive control group received CP 24 hours prior to sacrifice.
Examinations
- Tissues and cell types examined:
- Bone marrow from both femurs.
- Details of tissue and slide preparation:
- On the day of the last treatment, the animals were sacrificed. The marrow from both femurs of each animal was aspirated and flushed into fetal bovine serum. The marrow button was collected by centrifugation at approximately 200 x g for 5 minutes. Most of the supernatant was removed, and the cells were resuspended in the remaining 1-2 drops of serum. An automatic blood smearing instrument was used to make the bone marrow smears. At least 2 slides per animal were prepared and fixed in absolute methanol. The slides were stained with acridine orange in sodium phosphate buffer.
Method of analysis: Representative slides from each animal were examined in a blind manner using epifluoresence microscopy at a magnification of 630X. A minimum of one thousand polychromatic erythrocytes (PCEs) were scored for the presence of micronuclei. The number of micronucleated normochromatic erythrocytes (NCEs) seen in the optic fields were scored to assure that at least 1000 PCEs were also recorded. In addition, the ratio of polychromatic to normochromatic erythrocytes (NCEs) was determined. - Evaluation criteria:
- No data
- Statistics:
- Data for the percent micronucleated PCEs and proportion of PCEs among total erythrocytes were transformed prior to analysis using the arcsine square-root function. Weight gain data were not transformed. Data for each sex were analyzed separately by a one-way Analysis of Variance (ANOVA). The positive indicator group was not included in the analysis for effects of the test substance. All analyses were one-tailed.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- A significant decrease in body weight gain occurred in all groups of 1,3-dichlorobut-2-ene exposed male animals after 10 days of exposure.
- Vehicle controls validity:
- valid
- Negative controls validity:
- not examined
- Positive controls validity:
- valid
Any other information on results incl. tables
Analytical analysis of the concentrations: the test chamber concentrations were within normal range and delivered the approximate doses of 0, 10, 50, and 99 ppm 1,3-dichlorobut-2-ene.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
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