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EC number: 209-939-2 | CAS number: 598-55-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From December 4, 1986 to December 8, 1986
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to OECD Guideline 201, in compliance with GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 987
- Report date:
- 1987
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Methyl carbamate
- EC Number:
- 209-939-2
- EC Name:
- Methyl carbamate
- Cas Number:
- 598-55-0
- Molecular formula:
- C2H5NO2
- IUPAC Name:
- methyl carbamate
- Test material form:
- other: solid
Constituent 1
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Organism:Selenastrum capricornutum
- Strain no. 22662
- Source: American type culture collection
Study design
- Test type:
- static
- Total exposure duration:
- 96 h
Test conditions
- Test temperature:
- 21 - 22°C
- pH:
- 6.4 - 6.7
- Nominal and measured concentrations:
- 0, 180, 320, 560, 1,000 and 1,800 ppm
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass capped flasks
- Initial cells density: 1x10^4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Shaking culture was used for incubation
GROWTH MEDIUM
- Standard medium used: Sterile OECD Algal Medium (100 mL/flask)
Special Preparation/ Precautions:
(1)Sample diluted in distilled water v/v was stored in dark until used.
(2)Test concentration was established with pipettes and direct addition of dilutions
OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 8000 lux - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 414 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Correlation coefficient r^2 = 0.69
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 512 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Correlation coefficient r^2 = 0.89
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 598 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Correlation coefficient r^2 = 0.97
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 126 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Correlation coefficient r^2 = 0.69
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 232 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Correlation coefficient r^2 = 0.89
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 334 other: ppm
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: Correlation coefficient r^2 = 0.97
Any other information on results incl. tables
Table 1: Number of cells per mL x 104
|
48 h |
72 h |
96 h |
||||||
|
1 |
2 |
3 |
1 |
2 |
3 |
1 |
2 |
3 |
Control |
55 |
43 |
45 |
131 |
110 |
128 |
292 |
280 |
252 |
x |
48 |
123 |
270 |
||||||
180 ppm |
26 |
28 |
35 |
117 |
123 |
140 |
310 |
320 |
268 |
x (%I) |
30 (38%) |
127 (0%) |
299 (0%) |
||||||
320 ppm |
39 |
38 |
36 |
90 |
121 |
104 |
220 |
280 |
312 |
x (%I) |
38 (22%) |
105 (15%) |
270 (0%) |
||||||
560 ppm |
40 |
40 |
15 |
80 |
65 |
88 |
180 |
182 |
174 |
x (%I) |
32 (35%) |
78 (37%) |
179 (34%) |
||||||
1,000 ppm |
Less than 10 |
No viable cells |
No viable cells |
||||||
x (%I) |
>79% |
100% |
100% |
||||||
1,800 ppm |
No viable, countable cells |
Repeat: 1,000 ppm confirmed lack of growth confirmed
Note: Inoculum of 1 x104 cells/mL at "0" time; cell counts at 24 h recorded as less than 10x104 for all flasks. Cell growth was insufficient at 24 h to establish concentration effect relationships for all concentrations and for the blank control. The median effect, therefore, could not be calculated for that period.
x- Mean
%I - percent inhibition relative to control per time period
pH =pH of all flasks ranged 6.4 - 6.7, including control at start and finish of test.
As indicated by the calculated correlation coefficient above, the better estimate of the median algal inhibitory concentration was derived from 72 - 96 h data. This was due to more developed cell growth with time and thus better enumeration and differentiation among test concentrations
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 h, 72 h and 96 h EbC50 of test substance for Selenastrum capricornutum were 414, 512 and 598 ppm. The 48 h, 72 h and 96 h NOEC for effect on algal growth was determined to be 126, 232 and 334 ppm.
- Executive summary:
A study was conducted to evaluate the toxicity of the test substance to algae Selenastrum capricornutum under static conditions according to OECD Guideline 201, in compliance with GLP. On basis of preliminary range finding study, test concentrations were selected and test organisms were exposed to 0, 180, 320, 560, 1,000 and 1,800 ppm in 3 replicates per concentration. Incubation was done at 21 – 22⁰C in a shaking culture. Cell growth was insufficient at 24 h to establish concentration effect relationships for all concentrations and for the blank control. The median effect, therefore, could not be calculated for that period. Due to more developed cell growth with time and thus better enumeration and differentiation among test concentrations, the better estimate of the median algal inhibitory concentration was derived from 72 - 96 h data. All the validity criteria were met in this test. Percent growth was plotted versus log of concentration. Linear regression analysis was used to calculate EbC50 and NOEC. Under the study conditions, the 48 h, 72 h and 96 h EbC50 of test substance for Selenastrum capricornutum were 414, 512 and 598 ppm. The 48 h, 72 h and 96 h NOEC for effect on algal growth was determined to be 126, 232 and 334 ppm (Drozdowski D, 1987).
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