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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Remarks:
- no analytical monitoring
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-27 (Pseudomonas putida Zellvermehrungshemmtest)
- Version / remarks:
- Nov. 1992
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- Preparation of a slight turbid stock suspension with nominal concentration of 1000 mg product/l dilution water. The corresponding volumes were taken under permanent stirring and pipetting into the test vessels.
- Test organisms (species):
- Pseudomonas putida
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Hardness:
- -
- Test temperature:
- 20.8 - 21.0 °C
- pH:
- -
- Dissolved oxygen:
- -
- Salinity:
- -
- Nominal and measured concentrations:
- Nominal concentrations: control, 10, 30, 100 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: 100 ml Erlenmeyer flask
- Fill volume: 100 ml
- Aeration: directly
- No. of vessels per concentration (replicates): 2
- No. of blank values (replicates): 4
- Date of test: 1998-04-08
-Age of bacteria suspension: 2 days
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dilution water according to DIN 38412, part 27, 8.1, deviation: stock solution contained 33.3 mg Na2HPO4 x H2O /l and 0.5 g NH4Cl/l. To dilution water aliquots of test substance solution and 2 ml glucose solution and 1-3 ml bacterial suspenson were added. - Reference substance (positive control):
- no
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 60 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC0
- Effect conc.:
- 24 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- 29.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- Results on nominal effect concentrations (80 % a.i.)
30 min EC50: 75 mg/L, test mat.
30 min EC0: 30 mg/L, test mat.
30 min EC10: 37 mg/L, test mat.
Results on nominal effect concentrations (acitve ingredient)
30 min EC50: 60 mg/L, nominal
30 min EC0: 24 mg/L, nominal
30 min EC10: 29.6 mg/L, nominal - Validity criteria fulfilled:
- yes
- Conclusions:
- In this study with test substance fully saturated TEA-Esterquat the acute bacterial toxcitiy was determined according to DIN 38412, part 27 (Inhibition of oxygen demand (Sauerstoffzehrungshemmtest). The calculated effect concentrations after 30 minutes were as follows: EC0: 24.0 mg a.i./l, EC10: 29.6 mg a.i./l, EC50: 60 mg a.i./l.
Sample preparation and testing process following close after the other. We assume that hydrolysis can be neglected due to the short duration of test (30 min). Therefore the nominal concentration can be accepted as effect concentration, and reliability can be assigned as 2. - Executive summary:
In a study with test substance fully saturated TEA-Esterquat the acute bacterial toxicity towards Pseudomonas putida was determined under static conditions according to DIN 38412, part 27 (Inhibition of oxygen demand (Sauerstoffzehrungshemmtest) at test material concentrations of 10, 30, 100 mg test mat./L. The calculated effect concentrations after 30 minutes were as follows: EC0: 24.0 mg a.i./l, EC10: 29.6 mg a.i./l, EC50: 60 mg a.i./l.
This toxicity study is classified as reliable with restrictions and satisfies the guideline requirements for a respiration inhibition test according to DIN 38412, part 27.
Results Synopsis
Test Organism:Pseudomonas putida
Test Type (Static): DIN 38412, part 27.
30 min EC0: 24.0mg a.i./L
30 min EC10: 29.6 mg a.i./L
30 min EC50: 60 mg a.i./L
Endpoint(s) Effected: Respiration rate
Reference
Table 1: O2 decrease after 30 minutes
blank value |
Conc. mg prod./l |
Replicate |
mg O2/(l x min) |
1 |
|
|
1.73 |
2 |
|
|
1.83 |
|
10 |
Replicate 1 |
1.83 |
|
Replicate 2 |
1.81 |
|
|
30 |
Replicate 1 |
1.71 |
|
Replicate 2 |
1.72 |
|
|
100 |
Replicate 1 |
0.52 |
|
Replicate 2 |
0.54 |
|
3 |
|
|
1.79 |
4 |
|
|
1.84 |
Table 2: Inhibition of O2 decrease (mean values related of blank values)
Conc. mg prod./l |
mean value inhibition (+), increase (-) form 2 replicates |
10 |
- 1.1 |
30 |
4.6 |
100 |
71 |
Description of key information
30 min EC10 = 29.6 mg a.i./L; 30 min EC50 = 60 mg a.i./L (DIN 38412, part 27); GLP, RL1; read-across: fully saturated TEA-Esterquat
Key value for chemical safety assessment
- EC50 for microorganisms:
- 60 mg/L
- EC10 or NOEC for microorganisms:
- 29.6 mg/L
Additional information
No experimental data on the toxicity to microorganisms are available for the target substance C18 and C18 unsatd. TEA-Esterquat. However, reliable relevant data are available for the closely related source substances partially unsaturated TEA-Esterquat and fully saturated TEA-Esterquat.
A justification for read-across is given in the Endpoint Summary “Aquatic toxicity”.
In a study with fully saturated TEA-Esterquat the acute bacterial toxicity towards Pseudomonas putida was determined under static conditions according to DIN 38412, part 27 (Inhibition of oxygen demand (Sauerstoffzehrungshemmtest) at test material concentrations of 10, 30, 100 mg test mat./L. The calculated effect concentrations after 30 minutes were as follows: EC0: 24.0 mg a.i./L, EC10: 29.6 mg a.i./L, EC50: 60 mg a.i./L.
Supporting data are available for partially unsaturated TEA-Esterquat:
In a 3 hour toxicity study conducted according to OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test) and EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test), the cultures of activated sludge of a predominantly domestic sewage treatment plant were exposed to partially unsaturated TEA-Esterquat (CAS No. 157905-74-3) at nominal concentrations of 0, 1, 3, 9, 27, 81, 243 mg/L under static conditions. There was no inhibitory effect of the test item at any concentration; the deviations from the untreated controls were less than 15 %, both after 30 min and 3 hours. The 3 h EC50 value based on inhibition of respiration was > 243 mg/L.
The toxic effect of partially unsaturated TEA-Esterquat on Pseudomonas putida was assessed by measuring the respiration rate under defined conditions in the presence of different concentrations of partially unsaturated TEA-Esterquat (solvent free, 100 % a.i.) according to DIN 38412, part 27, 1992 (Inhibition of oxygen demand (Sauerstoffzehrungshemmtest)) at nominal concentrations of control, 10, 30 100, 300 1000, 3000, 10000 mg a.i./L. The calculated effect concentrations were as follows: 30 min EC0: 30 mg/L, 30 min EC10: 35 mg/L, and 30 min EC50 110 mg/L.
The toxic effect of partially unsaturated TEA-Esterquat on activated sludge from a biological treatment plant was assessed by measuring the respiration rate under defined conditions in the presence of different concentrations of the test article. According to OECD guideline 209 a concentration range between 16 and 125.0 mg/L of the test article was used in the test. Concentrations of 250 mg/L and 125 mg/L supported the respiration rates. Values of 2.6 % and 17.9 % compared to the untreated controls were obtained. Inhibition of the respiration rates were obtained with concentrations of 62, 32, and 16 mg/L of the test article and were determined to be 2.6 %, 7.7 %, and 7.7 %, respectively, compared to the untreated control. From the results, it was concluded to state that the test article did not severely inhibit the respiration rate of activated sludge obtained from a biological treatment plant. The stock solution of the test substance was prepared immediately before the test. The EC50 of the test substance is higher than 250 mg/L.
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