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EC number: 930-964-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: OECD guideline and GLP. There are some minor deviations which do not affect the outcome of the study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Deviations:
- yes
- Remarks:
- (only onde dose level was used and only 1000 erythrocytes were counted per animal instead of 2000).
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- - Name of test material (as cited in study report): monochloressigsäuremethylester
- Physical state: clear, colourless liquid
- Analytical purity: 99.4%
- Lot/batch No.: Tank from January 25th, 1988
- Storage condition of test material: dark at 4 ºC
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hoechst AG, Kastetngrund, SPF breeding colony
- Age at study initiation: 7 weeks
- Weight at study initiation: males: 26-33 g; females: 21-27 g
- Housing: in fully air-conditioned rooms in Macrolon cages (type 3), on softwood granulate in groups of five animals
- Diet (e.g. ad libitum): rat/mice diet Altromin 1324 (Altromin-GmbH, Lage/Lippe), ad libitum
- Water (e.g. ad libitum): tap water in plastic bottles, ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2 ºC
- Humidity (%): 55 ± 10%
- Photoperiod (hrs dark / hrs light): 12 hours light daily
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle(s)/solvent(s) used: sesame oil
- Details on exposure:
- PREPARATION OF DOSING SOLUTIONS: The test compound dilutions were prepared fresh each day. 750 mg of the test substance were weight in a beaker, mixed with sesame oil, washed out in a 25 ml flask and topped up to the calibration mark.
- Duration of treatment / exposure:
- Animals were treated with the test substance once and the animals were killed at 24, 48 and or 72 hours after treatment.
- Frequency of treatment:
- Single dose
- Post exposure period:
- Animals were killed at 24, 48 and or 72 hours after treatment.
Doses / concentrations
- Remarks:
- Doses / Concentrations:
300 mg/kg bw
Basis:
actual ingested
- No. of animals per sex per dose:
- 5 animals per sex per dose
- Control animals:
- yes
- Positive control(s):
- - Cyclophosphamid (Endoxan)
- Route of administration: orally
- Doses / concentrations: 50 mg/kg bw
Examinations
- Tissues and cell types examined:
- 1000 polychromatic erythrocytes were counted for each animal. The number of cells with micronuclei was recorded, not the number of individual micronuclei. The ratio of polychromatic to normochromatic erythrocytes was determined.
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION: The dose levels were selected on the basis of a preliminary study to determine the acute toxicity and the maximal applicable dose. Oral administration of 400 mg/kg bw caused partial lethality in male and female mice. The highest sublethal dose of 300 mg/kg bw was selected.
DETAILS OF SLIDE PREPARATION: Staining procedure:
5 minutes in methanol
3 minutes in May-Grünwalds solution
2 minutes in May-Grünwalds solution diluted 1:1 with distilled water
brief rinsing twice in distilled water
10 minutes staining in 1 part Giemsa solution to 6 parts buffer solution
rinsing with distilled water
drying
coating with Entellan - Evaluation criteria:
- All statistical results are based on a 95% level of significance.
- Statistics:
- Comparision of dose groups with the simultaneous control group was performed according to Wilcoxon (paired, one-sided, increase).
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- (death of 4 males and 2 females out of 70 animals. These animals were replaced).
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
It can be stated that, under the conditions described, administration of Monochloressigsäuremethylester did not lead to a substantial increase of micronucleated polychromatic erythrocytes. It is concluded that the test substance is not mutagenic in the micronucleus test. - Executive summary:
Methyl chloroacetate was tested in the micronucleus test. The test substance was administered orally by gavage to male and female NMRI mice at a dose of 300 mg/kg bw. The animals were treated once and killed at 24, 48 or 72 hours after treatment. The incidence of micronucleated polychromatic erythrocytes in the treated animals was within the normal range of the negative control. The number of normochromatic erythrocytes containing micronuclei was not increased. The ratio of polychromatic/normochromatic erythrocytes in both male and female animals remained unaffected by the treatment. It can be stated that, under the conditions described, administration of Monochloressigsäuremethylester did not lead to a substantial increase of micronucleated polychromatic erythrocytes. It is concluded that the test substance is not mutagenic in the micronucleus test.
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