Acetic anhydride

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented study report.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1980

Report date:

1980

Materials and methods

Principles of method if other than guideline:

BASF Test:
Young adult laboratory mice were used. The concentrations of the test substance were applied as a single interperitoneal dose. The post-exposure period was 14 days, after which the animals were sacrificed and subjected to gross-pathological examination. Groups of 5 male and 5 female mice were exposed to concentrations of the test substance as specified. Clinical symptoms were recorded immediately following injection, and 15 min, 30 min, 1 h, 2 h, 4 h, 5 h, 7 d and 14 d thereafter.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Acetic anhydride

EC Number:

203-564-8

EC Name:

Acetic anhydride

Cas Number:

108-24-7

Molecular formula:

C4H6O3

IUPAC Name:

acetyl acetate

Details on test material:

- Name of test material (as cited in study report): Acetic anhydride
- Analytical purity: approx. 98%

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Firma Wiga, Sulzfeld
- Age at study initiation: not recorded
- Weight at study initiation: males 28 g, females 27 g (mean)
- Fasting period before study: approx. 16 hours
- Diet (ad libitum): Herilan MHR-Kraftfutter
- Water (ad libitum): yes

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

olive oil

Doses:

46.4, 68.1, 100, 147 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 50 mg/kg bw

Mortality:

No animals died in any dose group 1 hour after substance application. In the lowest dose group, 1/5 male animals were found dead after 24 h and 2/5 males and 1/5 females, 3/5 males and 1/5 females, 3/5 males and 1/5 females, respectively, after 48 h, 7 days and 14 days. In the 68.1 mg/kg bw dose group, 1/5 males was found dead after 48 h, whereas 4/5 males and 4/5 females died after 7 days; after 14 days all animals had died. In case of the 100 mg/kg bw group, 2/5 males died after 24 h, 4/5 males and 2/5 females after 48 h and 5/5 males and 5/5 females after 7 days. 5/5 males and 3/5 females were found dead after 24 hours and all animals of that dose group had died after 48 h.

Clinical signs:

The animals of all dose groups showed dyspnea, apathy, staggering, paresis of the hind legs, twitching, tremor, spastic movements, convulsions in varying degrees and deteriorated general state.

Body weight:

not recorded

Gross pathology:

Gross pathological examination revealed an agglutinated liver in case of the two low dose groups and intraabdominal substance precipitation in case of one male of the 68.1 mg/kg bw dose group, but not in case of the two female animals of the same dose group; substance precipitation was also found in animals of the 100 mg/kg bw dose group. Both sexes showed comparable results.

Any other information on results incl. tables

Dose(mg/kg bw)	Mortality Dead/treated
	1 hour		24 hours		48 hours		7 days		14 days
	male	female	male	female	male	female	male	female	male	female
46.4	0/5	0/5	1/5	0/5	2/5	1/5	3/5	1/5	3/5	1/5
68.1	0/5	0/5	0/5	0/5	1/5	0/5	4/5	4/5	5/5	4/5
100	0/5	0/5	2/5	0/5	4/5	2/5	5/5	5/5	5/5	5/5
147	0/5	0/5	5/5	3/5	5/5	5/5	5/5	5/5	5/5	5/5