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EC number: 200-386-2 | CAS number: 58-56-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26-27 May 2002
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to OECD 209 (1984) and in compliance with GLP. The study report was very minimal and lacked detailed information. It was therefore considered reliable with restrictions.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984
- Deviations:
- yes
- Remarks:
- see below
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Version / remarks:
- 1986 (E) Method B
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Annex of EEC Directive 88/302/EEC (18 November 1987)
- Deviations:
- not specified
- Principles of method if other than guideline:
- The concentration of inoculum is somewhat lower than in the guideline (1.5 g/L). This is not causing and effect on the study as it is deemed worst-case.
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- No data. The stock solution was 2 g/L.
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Activated sludge from laboratory wastewater plant treating municipal sewage.
The inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated during the night. 50 mL were added to a total volume of 250 mL to obtain a concentration of 1 g/L dry substance in the test. - Test type:
- static
- Water media type:
- not specified
- Limit test:
- yes
- Total exposure duration:
- 30 min
- Post exposure observation period:
- 6 minutes
- Hardness:
- no data
- Test temperature:
- 20 ± 2 ºC
- pH:
- The stock solution of the test substance was corrected from pH 3.5 to pH 7.5. The stock solution of the reference substance was corrected from pH 5.6 to pH 7.3 (no further details given).
At t=0, before adding the inoculum, pH of blanks were 6.8 before correction and 7.1 after correction (no further details were given on correction). The pH in the test substance solution was 7.0 before adding the inoculum and 7.3 at 30 minutes. - Dissolved oxygen:
- Oxygen concentration during the 30 minutes aeration was > 2.5 mg/L
Oxygen concentration immediately before measurement was > 6.5 mg/L
See the attached table 1 of the oxygen concentrations during the 6 minutes measurement. - Salinity:
- Not applicable
- Nominal and measured concentrations:
- Limit test of 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL Erlenmeyer
- Aeration: yes, but no further details
- blank control replicates: 3
- test substance replicates: 1
- reference substance replicates: 1
- Biomass loading rate: 1 g/L (50 mL inoculum/vessel)
TEST MEDIUM / WATER PARAMETERS
- 8 ml/vessel 100-fold concentrated OECD synthetic medium (corresponding to the OECD 209, 1984)
OTHER TEST CONDITIONS
- Adjustment of pH: Yes
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Oxygen concentration after 30 minutes incubation and 6 minutes later.
TEST CONCENTRATIONS
- Limit test of 1000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The test substance had no effect on the respiration rate of activated sludge as compared to the blank control.
- Results with reference substance (positive control):
- See the details in the attached table 3. The EC50 was 15 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the test, the test substance had no effect on the respiration rate of activated sludge as compared to the blank control. The EC50 was > 1000 mg/L. The NOEC was = 1000 mg/L
- Executive summary:
An activated sludge respiration inhibition test was conducted with Vitamin B6 hydrochlorid according to OECD 209 (1984) and in compliance with GLP. After 30 minutes of exposure, the test substance did not cause effects on the respiration rate of activated sludge as compared to the blank control. The EC50 was > 1000 mg/L and the NOEC = 1000 mg/L. The validity criteria of the guideline were fulfilled. The study report was very minimal and lacked detailed information. The study was thus considered to be reliable with restrictions.
Reference
Description of key information
An activated sludge respiration inhibition test was conducted with Vitamin B6 hydrochlorid according to OECD 209 (1984) and in compliance with GLP. After 30 minutes of exposure, the test substance did not cause effects on the respiration rate of activated sludge as compared to the blank control. The EC50 was > 1000 mg/L and the NOEC = 1000 mg/L. The validity criteria of the guideline were fulfilled. The study report was very minimal and lacked detailed information. The study was thus considered to be reliable with restrictions.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
Additional information
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