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Diss Factsheets
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EC number: 269-905-8 | CAS number: 68390-58-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- (the pH reference substance stock solution was adjusted to 6.75, which is slightly outside the requested range of 7.5±0.5. Since the validity criterion regarding the reference substance was met the integrity or validity of the study was not affected.)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- not required
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance is poorly soluble in water. Weights of the test material were added directly to the test bottles followed by RO (reverse osmosis) water (284 mL), synthetic sewage (16 mL) and antifoam agent (silicone oil in water, 100 µL). Immediately prior to initiation of the respirometer, the required volume of activated sludge (200 mL) was added to each bottle.
- Controls: RO water, synthetic sewage and antifoam agent alone.
The final volume of antifoam was not considered significant and was not accounted for in the final overall volume (500 mL). - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Preparation of inoculum for exposure: Activated sludge was obtained the day before the start of the test. The mixed liquor suspended solids (MLSS) content was determined, synthetic sewage (50 mL/L) was added and the mixture was aerated overnight. On the day of the test, the MLSS content of the sludge was determined and adjusted to 4 g/L by the addition of tap water.
- Pretreatment: none
- Initial biomass concentration: 4 g MLSS/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21°C ± 0.4°C
- pH:
- test start: 7.4-7.7 (adjusted using 0.1M NaOH as required)
test end: 7.8-8.0 - Dissolved oxygen:
- 3.7 - 6.6 mg O2/L (initial measured dissolved oxygen concentration in the cultures)
- Nominal and measured concentrations:
- nominal 10, 100, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test instrument: automated respirometer and associated software (Coordinated Environmental System (CES).
- Test vessel: 500 mL bottles fitted with an oxygen probe, a sinter to deliver air and a nitrogen gas nozzle
- Aeration: oil-free compressed air at 1 L/min
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
- Biomass loading rate: 1.6 g/L (MLSS)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reverse osmosis (RO) water
OTHER TEST CONDITIONS
- Adjustment of pH: to 7.0-8.0 with 0.1M NaOH as required
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen concentration after 3 hours contact time
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Range finding study/limit test
- Test concentrations: 10, 100, 1000 mg/L
- Results used to determine the conditions for the definitive study: As there was no significant inhibition observed in any test groups, the study was reported as a limit test. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The mean inhibition observed in cultures with the test substance ranged between -11 and -2% (i.e. slight stimulation) with ranges of -26 to 30% (coefficient of variation 7 - 19%; see Table 1).
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 (3 h): 11.4 mg/L (95% confidence limits 2.7-47.3 mg/L) - Reported statistics and error estimates:
- EC calculation: A logistic sigmoid curve was fitted to the data by Marquardt's iterative compromise method.
NOEC calculation: The F1 approximate test for monotonicity of dose-response was applied. Since it was not significant at the 1% level, the Williams' test was performed.
Test and reference substance were analysed separately.
Data were analysed using SAS 9.1.3, Proc NLIN (SAS Institute, 2002). - Validity criteria fulfilled:
- yes
- Remarks:
- The mean specific respiration rate of the blank controls was ≥20 mgO2/gh with a coefficient of variation of ≤30%. The EC50 of 3,5-dichlorophenol was in the accepted range of 2-25 mg/L.
Reference
Table 1 Specific respiration rates, inhibition and coefficient of variation
Group | Nominal Conc. (mg/L) | Bottle No. | Specific respiration rate (Rs mgO2/gh) | Mean Rs | % inhibition | Mean % inhibition | Coefficient of variation (%) |
|
|
|
|
|
|
|
|
Control | - | 1 | 31.0 | 26.7 | - | - | 23 |
2 | 28.7 | ||||||
3 | 29.2 | ||||||
4 | 28.7 | ||||||
5 | 15.8 | ||||||
Test Substance | 10 | 6 | 26.4 | 29.4 | 1 | -10 | 7 |
7 | 29.0 | -9 | |||||
8 | 29.4 | -10 | |||||
9 | 31.2 | -17 | |||||
10 | 31.3 | -17 | |||||
100 | 11 | 26.8 | 27.2 | 0 | -2 | 19 | |
12 | 18.8 | 30 | |||||
13 | 27.9 | -5 | |||||
14 | 33.1 | -24 | |||||
15 | 29.6 | -11 | |||||
1000 | 16 | 32.7 | 29.6 | -23 | -11 | 13 | |
17 | 29.6 | -11 | |||||
18 | 33.6 | -26 | |||||
19 | 24.0 | 10 | |||||
20 | 28.1 | -5 |
The data presented were calculated using unrounded values stored in the computer database. Minor numerical differences may be observed in the respiration rate calculation if rounded values are used to calculate the data. This minor discrepancy is not considered to be significant
Negative inhibition indicates a respiration rate greater than that of the mean control respiration rate
Description of key information
Toxicity to microorganisms: no inhibition of respiration rates (OECD 209)
Key value for chemical safety assessment
Additional information
EC50 for microorganisms > 1000 mg/L
EC10 or NOEC for microorganisms ≥ 1000 mg/L
The results indicate that the test material is not toxic to sewage bacteria up to the limits of its water solubility (<1 mg/L) and a NOEC and thus a PNEC cannot be determined.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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