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Diss Factsheets
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EC number: 209-057-8 | CAS number: 554-00-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics in vivo
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: significant data on methemoglobin formation although reported in brief
Data source
Reference
- Reference Type:
- publication
- Title:
- Methemoglobin formation by aromatic amines.
- Author:
- McLean S, Starmer GA, Thomas J
- Year:
- 1 969
- Bibliographic source:
- J Pharm Pharmac 21, 441-450
Materials and methods
- Objective of study:
- other: determination of methemoglobinemia
- Principles of method if other than guideline:
- 5 cats received oral dose of 0.25 mmol/kg bw once and the amount of methemoglobin formation was measured over a period of 5 hours
- GLP compliance:
- no
Test material
- Reference substance name:
- 2,4-dichloroaniline
- EC Number:
- 209-057-8
- EC Name:
- 2,4-dichloroaniline
- Cas Number:
- 554-00-7
- Molecular formula:
- C6H5Cl2N
- IUPAC Name:
- 2,4-dichloroaniline
- Test material form:
- not specified
- Details on test material:
- no data
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- cat
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- as discribed in McLean J Pharm Pharmac 19, 146-154 (1967)
adult cats, older than 24 weeks, were used
food was witheld from animals for 16 hours before a start of an experiment
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: 1% methylcellulose mucilage
- Details on exposure:
- 5 cats received oral dose of 25 mmol/kg bw dissolved in 1% methylcellulose mucilage once and the amount of methemoglobin formation was measured over a period of 5 hours
- Duration and frequency of treatment / exposure:
- once
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0.25 mmol/kg bw
- No. of animals per sex per dose / concentration:
- 5
- Control animals:
- not specified
- Positive control reference chemical:
- no data
- Details on study design:
- adult cats, older than 24 weeks, were used
food was witheld from animals for 16 hours before a start of an experiment
5 cats received oral dose of 0.25 mmol/kg bw in 1% methylcellulose mucilage once and the amount of methemoglobin formation was measured by taking blood from femoral vein just before and at 1 h -intervals after treatment up to 5 hours. - Details on dosing and sampling:
- 5 cats received oral dose of 0.25 mmol/kg bw in 1% methylcellulose mucilage once and the amount of methemoglobin formation was measured over a period of 5 hours. Blood was taken from femoral vein just before and at intervals after treatment
- Statistics:
- mean values from 5 cats
Results and discussion
Main ADME results
- Type:
- other: determination of methemoglobin
- Results:
- Mean methemoglobin percentage formed after 5 hours was 31.7 % with a mean max of42.0 %
Toxicokinetic / pharmacokinetic studies
- Details on absorption:
- no details
- Details on distribution in tissues:
- no details
- Details on excretion:
- no details
Metabolite characterisation studies
- Details on metabolites:
- no data
Any other information on results incl. tables
0.25 mmol/kg bw to 5 cats; mean value of methemoglobin formation
after 1h: 39.3 %;
after 2 h: 37.3 %;
after 3 h: 31.5%;
after 4 h: 27.6 %;
after 5 h: 22.6 %
Applicant's summary and conclusion
- Executive summary:
5 cats received oral dose of 0.25 mmol/kg bw in 1% methylcellulose mucilage once and the amount of methemoglobin formation was measured over a period of 5 hours. Blood was taken from femoral vein just before and at intervals after treatment. Mean methemoglobin percentage formed after 5 hours was 31.7 % with a mean max of 42.0 %
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