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EC number: 203-273-6 | CAS number: 105-13-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2016-04-13 to 2016-04-15
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 2004
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: control; 100 mg/L (diluted by factor 50 prior to analysis)
- Sample storage conditions before analysis:All samples were stored at 6 ± 2 °C until preparation, if necessary. Prepared samples were stored at room temperature in the autosampler until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test item was melted prior to the start of the exposure at 30°C. The limit concentration (100.3 mg of the test item was weighed out) was freshly prepared with dilution water in a measuring with a volume of 1 L before the start of the exposure (0 hours). The limit concentration was mixed thoroughly by inverting the measuring flask 10 times per hand. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain/clone: STRAUS
- Age of parental stock: less than 24 hours
- Feeding during test: none
ACCLIMATION
- not necessary because the dilution water was equivalent to the culture medium - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 166 - 170 mg CaCo3/L
- Test temperature:
- 20.3 - 20.5°C
- pH:
- 7.33 - 8.50
- Dissolved oxygen:
- 7.12 - 8.67 mg/L
- Salinity:
- NA
- Conductivity:
- 491 - 502 µS/cm
- Nominal and measured concentrations:
- nominal concentration: 100 mg/L
measured concentration: 102 - 104 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers
- Type: loosely covered with watch glasses
- Material, size, headspace, fill volume: glass, 4 (ID) x 7 (H) cm, headspace, 50 mL capacity
- Renewal rate of test solution: The test solutions were renewed after 24 hours. A second set of test vessels were filled with the freshly prepared test solutions and the daphnids were transferred by pipette.
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4
TEST MEDIUM / WATER PARAMETERS
- Elendt M4 according to OECD 202 (modified to a total hardness of 160 and 180 mg CaCO3/L
- Intervals of water quality measurement: Prior to the start of the exposure (0 hours) and the water renewal (24 hours), the water quality parameters (i.e. pH-value, dissolved oxygen concentration, temperature, conductivity and total hardness) of the dilution water were measured. At the start of the exposure intervals (0 and 24 hours), the water quality parameters of the fresh media (i.e. pH-value, dissolved oxygen concentration) were measured in one additional replicate (without daphnids) of the limit concentration and the control. At the end of the exposure intervals (24 and 48 hours), the water quality parameters of the old media were measured in all replicates (containing daphnids) of the limit concentration and the control.
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: diffuse light, light intensity of max 1340 lux
EFFECT PARAMETERS MEASURED: Immobilisation was determined in all groups after 24 and 48 hours.
RANGE-FINDING STUDY
- Test concentrations: 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study: No immobilisation was observed up to and including the highest dose tested. Based on this result, a limit concentration of 100 mg/L was chosen for the main test. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichomate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Any observations that might cause a difference between measured and nominal values: no
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
A reference test was conducted as an acute immobilisation test (acc. to AQS P 9/2 and OECD 202) in ISO test water (acc. to OECD 202 (2004), Annex 3) under static conditions with a test duration of 24 hours once per month in order to prove the validity of the test system and test conditions at the test facility.The EC50 - value for the most recent of the monthly performed reference tests was: EC50: 1.95 mg/L (Cl: 1.14 - 3.30 mg/L) - Validity criteria fulfilled:
- yes
- Conclusions:
- In the limit concentration 100 mg/L of the test item, no effects on Daphnia magna were observed. Thus, the EC50 value is greater than 100 mg/L.
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the limit concentration 100 mg/L of the test item were determined according to OECD 202. The limit test was conducted with headspace under semi-static conditions over a period of 48 hours. The validity criteria of the guideline were fulfilled. In the limit concentration 100 mg/L, no effects on Daphnia magna were observed. Thus, the EC50 value of the test item is greater than 100 mg/L.
Reference
Table 1: Immobilisation Rates
Nominal concentration [mg/L] |
Immobilisation (%) |
|||||||||
24 hours |
48 hours |
|||||||||
Replicates |
Replicates |
|||||||||
1 |
2 |
3 |
4 |
mean |
1 |
2 |
3 |
4 |
mean |
|
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Table 2: Measured Concnetrations of the Test Item during the Definitive Test
|
0 hours (start of exposure interval) |
24 hours (end of exposure interval) |
24 hours (start of exposure interval) |
48 hours (start of exposure interval) |
||||
Nominal concentration [mg/L] |
Measured conc. [mg/L] |
% |
Measured conc. [mg/L] |
% |
Measured conc. [mg/L] |
% |
Measured conc. [mg/L] |
% |
100 |
102 |
102 |
101 |
101 |
104 |
104 |
101 |
101 |
control |
< LOQ |
< LOQ |
< LOQ |
< LOQ |
LOQ: Limit of Quantification
Description of key information
The acute immobilisation of the test item to daphnia magna was determined according to OECD guideline 202. The EC50 value after 48 h was determined to be greater than 100 mg/L.
Key value for chemical safety assessment
Additional information
In the acute immobilisation test with Daphnia magna (STRAUS), the effects of the limit concentration 100 mg/L of the test item were determined according to OECD 202. The limit test was conducted with headspace under semi-static conditions over a period of 48 hours. The validity criteria of the guideline were fulfilled. In the limit concentration 100 mg/L, no effects on Daphnia magna were observed. Thus, the EC50 value of the test item is greater than 100 mg/L.
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