Phosphonium, (3-methyl-4-oxo-2-buten-1-yl)triphenyl-, chloride (1:1)

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: study performed according to OECD and GLP guidelines

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Phosphonium, (3-methyl-4-oxo-2-butenyl)triphenyl-, chloride

EC Number:

609-124-5

Cas Number:

3541-81-9

Molecular formula:

C23 H22 O P . Cl

IUPAC Name:

Phosphonium, (3-methyl-4-oxo-2-butenyl)triphenyl-, chloride

Method

Target gene:

TA97a: hisD6610, TA 98 TA1537, TA1538: hisD3052; TA 100 and TA 1535 hisG46, TA102: hisG428

Species / strain

Species / strain / cell type:

other: TA1535, TA1537, TA1538, TA97, TA98, TA100 and TA102

Additional strain / cell type characteristics:

other: rfa, delta-uvrB, pKM101

Metabolic activation:

with and without

Metabolic activation system:

S9

Test concentrations with justification for top dose:

0, 1, 3, 10, 33, 100, 333, 500, 1000 ug/plate

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

yes

Positive controls:

yes

Positive control substance:

2-nitrofluorene

sodium azide

other: 2-Aminoanthracene

Results and discussion

Test results

Species / strain:

other: TA1535, TA1537, TA1538, TA97, TA98, TA100 and TA102

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

cytotoxicity

Remarks:

starting at 3333 ug/plate

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

-

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

substance is not mutagenic

Executive summary:

Wittigaldehydechlorid was tested for mutagenic activity in the Ames test. A standard plate incorporation as well

as a preincubation modification assay were performed. All seven currently used standard tester strains (TA1535, TA1537, TA1538, TA97, TA98, TA100 and TA102) were evaluated in absence and in presence of a phenobarbital/p-naphthoflavone

induced rat liver homogenate fraction (S9-mix). Responsiveness of the tester strains and activity of the S9-mix were demonstrated by using appropriate positive controls for each specific strain.

The substance was dissolved in dimethylsulfoxide (DMSO). In a preliminary toxicity experiment the compound showed bacteriotoxicity starting at 3’333 ug/plate. Therefore for the main experiments the dose range 10 to 1’000 ug/plate was selected. Due to an even stronger toxic action in the preincubation assay which became apparent at 33 ug/plate a repeat experiment in the dose

range 0.33 to 33 ug/plate was performed. No precipitation of the compound was observed up to the chosen maximum test concentration. No increase in the number of his+ revertant colonies was observed for any of the seven tester strains investigated in this study.

Therefore it can be concluded, that neither Wittigaldehydechlorid per se, nor any of its metabolites formed under the described experimental conditions is mutagenic in the Salmonella/mammalian microsome assay (Ames test).