7-[{4-[(4-amino-6-chloro-1,3,5-triazin-2-yl)amino]-2-(carbamoylamino)phenyl}diazenyl]naphthalene-1,3,6-trisulfonic acid, lithium sodium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From November 24, 2015 to January 5, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test organisms (species):

Daphnia magna

Details on test organisms:

- Source: Aquatic BioSystems (Fort Collins, CO)

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

Hardness of 100 mg/L as CaCO3

Test temperature:

20.1-21.1°C

pH:

8.1-8.2

Nominal and measured concentrations:

Nominal concentrations of the Test Item were 6.25, 12.5, 25, 50 and 100 mg/L
Measured concentrations of the Test Item wre 7.8, 16.4, 31.3, 63.6 and 132.6 mg/L

Details on test conditions:

- Test vessel: ~250 mL glass beakers
- Test volume: 200 mL
- Aeration: None
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Photoperiod: 16-hr light: 8-hr dark with a 30 minute transition period
- Light intensity: 687-773 lux

Reference substance (positive control):

yes

Remarks:

Zinc sulphate heptahydrate (CAS No. 7446-20-0)

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 132.6 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

48-hr LC50: 0.35 mg Zn2+/L

The 0% of control organisms were immobilised and the dissolved oxygen concentration at the end of the test was 9.0-9.2 mgO2/L. The initial pH measurements did not differ from the final measurements by more than 1.5 units in each test.

Table 1. Number and Percentage of Daphnia Immobilised after 24-hr Exposure

Concentration of Test Item (mg/L)		Mean ± SD (%)	Replicate A (# / %)	Replicate B (# / %)	Replicate C (# / %)	Replicate D (# / %)
Nominal	Mean Measured
0 (Neg. Control)	0	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
6.25	7.8	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
12.5	16.4	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
25	31.3	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
50	63.6	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
100	132.6	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0

Table 2. Number and Percentage of Daphnia Immobilised after 48-hr Exposure

Concentration of Test Item (mg/L)		Mean ± SD (%)	Replicate A (# / %)	Replicate B (# / %)	Replicate C (# / %)	Replicate D (# / %)
Nominal	Mean Measured
0 (Neg. Control)	0	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
6.25	7.8	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
12.5	16.4	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
25	31.3	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
50	63.6	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0
100	132.6	0 ± 0	0 / 0	0 / 0	0 / 0	0 / 0

Table 3.Test Endpoint Summary for CJ312

Concentration (mg/L)	CJ312 (mg/L)
	48-hr EC50	LOEC	NOEC
Nominal	>100	>100	100
Mean Measured	>132.6	>132.6	132.6

Validity criteria fulfilled:

yes

Conclusions:

According to OECD 202 test method, based on the mean measured concentrations of CJ312: the 48-hr EC50 of CJ312 was >132.6 mg/L.

Executive summary:

This test using the procedures outlined in the Maxxam Study Plan for TOX1519 which is based on the SOP for the OECD 202 (BBY2SOP-00017) and OECD 202 (OECD, 2004). The results of this OECD 202 test for CJ312 show that all validity criteria were met.

Based on the mean measured concentrations of CJ312:

1.     The 48-hr EC50 was >132.6 mg/L.

2.     The NOEC and LOEC was 132.6 mg/L and >132.6 mg/L, respectively.

Description of key information

Based on the mean measured concentrations of CJ312:

The 48-hr EC50 of CJ312 was >132.6 mg/L for Daphnia magna (OECD TG202).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

132.6 mg/L

Additional information

The test was considered valid as 0% of control organisms were immobilised and the minimum dissolved oxygen concentration at the end of the test was 9.0-9.2 mg O2/L. All test validity criteria were met. The pH of the test solutions was within the ranges outlined in the OECD 202 test guideline. Themean measured concentrationsof CJ312 were not within 80-120% of their nominal concentrations.

Based on the mean measured concentrations of CJ312:

1. The 48-h EC50 was >132.6 mg/L.

2. The No Observed Effect Concentration (NOEC) was 132.6 mg/L.

3. The Lowest Observed Effect Concentration (LOEC) was >132.6 mg/L.