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Diss Factsheets
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EC number: 300-644-5 | CAS number: 93951-21-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method of administration: Direct weighing
- Water for dilution: Synthetic fresh water in accordance with ISO.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
- Strain: HAMILTON BUCHANAN
- Source: Bio International B.V. (Netherlands)
- Age at study initiation (mean and range, SD): ~ 4 mo
- Length at study initiation (length definition, mean, range and SD): 2.5-3.5 cm
ACCLIMATION
- Acclimation conditions (same as test or not): Yes
- Keeping water: Synthetic fresh water in accordance with ISO
- Mortality: <2 % while kept - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No
- Hardness:
- 14.1 °dH
- Test temperature:
- 20.8-22 °C
- pH:
- 7.1-8
- Dissolved oxygen:
- 7.5-8.8 mg/L
Oxygen [% saturation]=86.5-101.3 - Salinity:
- No data
- Nominal and measured concentrations:
- Nominal: 1000 and 3160 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Test vessels (300 x 135 x 200 mm)
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 5 L test medium
- Aeration: Yes
- Type of flow-through (e.g. peristaltic or proportional diluter): Static
- Method of administration: Direct weighing
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic fresh water in accordance with ISO
OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality, visible effects on appearance and behaviour
TEST CONCENTRATIONS
- Test concentrations: 1000, 3160 mg/L - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: -
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1 777.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: geometric mean of LC0 and LC100
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 3 160 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: -
- Details on results:
- - Behavioural abnormalities: No effects at 1000 mg/L, at 3160 not observable due to staining of test medium
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the test conditions, the 96 h LC0 value of the test substance (including inorganic components) was determined to be 1000 mg/L.
- Executive summary:
A study was performed to assess the acute toxicity of test substance to Zebra fish (Brachydanio rerio) under static conditions. The study was conducted in accordance with EU Method C.1. in compliance with GLP.
Groups of ten fish of the recommended size were exposed to limit test concentration of nominally 1000 and 3160 mg/L of test substance dissolved in synthetic fresh water.
Observations were made on the number of dead fish and for the visible effects on appearance and behaviour of fish after 0, 24, 48, 72 and 96 h of exposure.
Reference
Description of key information
The 96 h LC0, LC 50 and LC100 values of the test substance were determined to be1000, 1777.6, and 3160 mg/L, respectively.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 1 777.6 mg/L
Additional information
A study was performed to assess the acute toxicity of test substance to Zebra fish (Brachydanio rerio) under static conditions. The study was conducted in accordance with EU Method C.1. in compliance with GLP.
Groups of ten fish of the recommended size were exposed to limit test concentration of nominally 1000 and 3160 mg/L of test substance dissolved in synthetic fresh water.
Observations were made on the number of dead fish and for the visible effects on appearance and behaviour of fish after 0, 24, 48, 72 and 96 h of exposure.
Under the test conditions, the 96 h LC0, LC 50 and LC100 values of the test substance were determined to be1000, 1777.6, and 3160 mg/L, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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