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Diss Factsheets
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EC number: 200-578-6 | CAS number: 64-17-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1995
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, study with sufficient basic documentation to demonstrate that study meets basic scientific principles and contains enough detail to be able to judge the results reliable as a supporting study.
Data source
Reference
- Reference Type:
- publication
- Title:
- The lethal effects of ethanol and cocaine and their combination in mice: implications for cocaethylene formation.
- Author:
- Schechter, M. and Meehan, S.
- Year:
- 1 995
- Bibliographic source:
- Pharmacol Biochem Behav 52(1):245-248.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The study examined the lethal effects of ethanol and cocaine and their use in combination by using percent mortality and the resultant LD50's as a measure of acute toxicity. The ethanol groups were the controls for the main experiment.
- GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- Ethanol
- EC Number:
- 200-578-6
- EC Name:
- Ethanol
- Cas Number:
- 64-17-5
- Molecular formula:
- C2H6O
- IUPAC Name:
- ethanol
- Details on test material:
- - Name of test material (as cited in study report): No data
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- other: HS
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 25 - 30 days old.
- Housing: Plexiglass cages with aspen shavings in a climate controlled room.
- Diet: ad libitum except during 2hr period post dosing.
- Water: ad libitum except during 2hr period post dosing.
ENVIRONMENTAL CONDITIONS
- Temperature and humidity climate controlled but not specified
- Photoperiod: 12 hour light/12 hour dark
NOTE: This strain is regarded as particularly suitable for testing with cocaine.
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- physiological saline
- Doses:
- - Doses: 6, 8 and 10 g/kg in 20% w/v solution in 0.9% physiological saline. A second injection of saline only was given immediately afterwards into the contralateral location (5ml/kg). This dose acted as the vehicle only control for the main experiments using cocaine.
- Doses per time period: One
- Exposure duration: not applicable. - No. of animals per sex per dose:
- 10
- Control animals:
- no
- Details on study design:
- - After injection, animals monitored for 2 hours for indications of toxicity and lethality for close observation before returning to home cages. Death defined as an absence of respiration for 30s or more.
- Duration of observation period following administration: 24 hours before sacrifice. - Statistics:
- Calculation of LD50 using a computerised version of the Litchfield-Wilcoxon analysis (
Results and discussion
Effect levelsopen allclose all
- Sex:
- female
- Dose descriptor:
- LC50
- Effect level:
- 9 450 mg/kg bw
- 95% CL:
- > 8 450 - < 10 490
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 9 710 mg/kg bw
- 95% CL:
- > 8 380 - < 11 270
- Mortality:
- All deaths occurred within 30 minutes. Individual times of death were not given.
- Clinical signs:
- Description, severity, time of onset and duration of clinical signs at each dose level were not described.
- Other findings:
- - Potential target organs: not discussed.
- Necropsy findings, included doses affected, severity and number of animals affected: not done.
Any other information on results incl. tables
The LD50 values were 838 to 1127 (9.71 g/kg) in males and 8.45 to 1049 g/kg (9.45 g/kg) in females.
Applicant's summary and conclusion
- Executive summary:
A study established that the LD50 of ethanol in mice when given by the ip route is 9710mg/kg for males and 9450mg/kg for females.
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