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EC number: 213-063-6 | CAS number: 921-03-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1987 to 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study comparable to OECD Guideline.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 988
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Deviations:
- no
- GLP compliance:
- not specified
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 1,1,3-trichloroacetone
- EC Number:
- 213-063-6
- EC Name:
- 1,1,3-trichloroacetone
- Cas Number:
- 921-03-9
- Molecular formula:
- C3H3Cl3O
- IUPAC Name:
- 1,1,3-trichloropropan-2-one
- Details on test material:
- as purchased by Aldrich Chemical Company, Milwaukee, WI, USA
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- Swiss Webster
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Simonsen Laboratories inc., Gilroy, CA, USA
- Age at study initiation: young adults
- Weight at study initiation: 20 to 30 g
- Assigned to test groups randomly: yes
- Fasting period before study: not reported
- Housing: 2-4/cage
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle(s)/solvent(s) used: 2% Castor oil, ethylene oxide reaction product (Emulphor EL-620), water
- Justification for choice of solvent/vehicle: solubility
- Concentration of test material in vehicle: depending on volume
- Amount of vehicle (if gavage or dermal): 1 ml/kg bw - Details on exposure:
-
PREPARATION OF DOSING SOLUTIONS: not reported - Duration of treatment / exposure:
- twice at beginning of test and 24 hours apart
- Frequency of treatment:
- 24 hours apart
- Post exposure period:
- 48/72 h
Doses / concentrations
- Remarks:
- Doses / Concentrations:
3, 6, 12 mg/kg bw
Basis:
nominal conc.
- No. of animals per sex per dose:
- 12 males and 12 females per dose (6 per sex to be evaluated after 48 and 6 to be evaluated 72 hours after treatment)
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- trimethylphosphate
- Justification for choice of positive control(s): not reported
- Route of administration: oral gavage
- Doses / concentrations:2 doses of 1250 mg/kg bw
Examinations
- Tissues and cell types examined:
- Femoral bone marrow
- Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION: toxicity of substance
TREATMENT AND SAMPLING TIMES ( in addition to information in specific fields):mice received 2 oral gavage administrations 24 hours apart; sampling time 48 and 72 hours after first treatment
DETAILS OF SLIDE PREPARATION:Bone marrow cell suspensions from both femurs were prepared in fetal bovine serum, snmeared on slides and stained in buffered Giemsa stain (pH 6.8). The prepared slides were coded aprior to analysis. At least 500 polychromatic errythrocytes (PCE)/animal were scored for the presence of micronuclei. The ratio of PCE to mature erythrocytes (RBC) was determined from the number of RBC encountered while scoring the first 200 PCE/animal - Evaluation criteria:
- micronucleated PCE/500 PCE > 0.90 (p < 0.05)
- Statistics:
- Method of Mackey and MacGregor
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- RESULTS OF RANGE-FINDING STUDY
- Dose range: not performed
RESULTS OF DEFINITIVE STUDY
- Induction of micronuclei (for Micronucleus assay): 0.04 to 0.18 %
- Ratio of PCE/NCE (for Micronucleus assay): 1.08 to 1.31
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
Substance is not clastogenic in vivo to bone marrow under the conditions used. - Executive summary:
1,1,3 -Trichloroacetone was assessed for its capacity to induce micronuclei in the femur bone marrow of male and female Swiss-Webster mice. The substance was given twice by oral gavage to 4 groups of 12 males and 12 females at concentrations of 0, 3, 6 and 12 mg/kg bw. Another group received 1250 mg/kg bw trimethylphosphate as a control which worked as expected. No effect was seen on cytotoxicity the PCE/RBC ratio nor on the number of polymicronucleated PCE. The substance is not clastogenic in vivo to bone marrow cells of mice under the conditions used.
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