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EC number: 202-905-8 | CAS number: 100-97-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: public available literature (non GLP, no guideline)
Data source
Reference
- Reference Type:
- publication
- Title:
- In vivo mammalian bone marrow cytogenetic test (chromosomal analysis) - Result of testing UROTOVET(R).
- Author:
- Vujosevic, M., Zivkovic, S., Fister, S.
- Year:
- 1 986
- Bibliographic source:
- Acta Vet (Beograd), 36, 91-94
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- According to the method of Hsu and Patton (1969).
- GLP compliance:
- not specified
- Type of assay:
- mammalian bone marrow chromosome aberration test
Test material
- Reference substance name:
- Methenamine
- EC Number:
- 202-905-8
- EC Name:
- Methenamine
- Cas Number:
- 100-97-0
- Molecular formula:
- C6H12N4
- IUPAC Name:
- 1,3,5,7-tetraazatricyclo[3.3.1.1³,⁷]decane
- Test material form:
- solid: crystalline
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- C3H
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 10-12 weeks old
- Weight at study initiation: 20-25 g
- Animals were kept under standard conditions of temperature and relative humidity.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- distilled water
- Details on exposure:
- Test substance was administered per os (presumably gavage).
- Duration of treatment / exposure:
- Acute study: 3 different sampling times after single exposure: 6, 12 and 24 h.
Repeated dose study: 5 days - Frequency of treatment:
- Acute study: single treatment
Repeated dose study: 5 exposures (24 h apart) - Post exposure period:
- Acute study: 3 different sampling times after single exposure: 6, 12 and 24 h.
Repeated dose study: Sampling time was 6 h after last exposure
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 68.6, 205.9, 617.7mg/kg bw
Basis:
actual ingested
- No. of animals per sex per dose:
- 5 male animals per dose and time point
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- yes, Ethyl-methane sulfonate (EMS) was used at a concentration of 50 mg/kg bw.
Examinations
- Tissues and cell types examined:
- mice bone marrow cells
- Details of tissue and slide preparation:
- Chromosome preparations were made according to the method of Hsu and Patton (1969). Slides were stained in 10% Giemsa. At each experimental point 300 cells were scored (60 per animal).
- Evaluation criteria:
- The basic chromosomal abnormalities that were considered included chromatid and chromosome breaks and exchanges. For comparative purposes the basic index used was the number of breaks per metaphase (B/C).
- Statistics:
- not indicated.
Results and discussion
Test results
- Key result
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- During the course of the experiment no animal died. No chromosome aberrations of the exchange type were observed in any of the test substance treated groups.
Applicant's summary and conclusion
- Conclusions:
- It can be concluded that under the testing conditions, the test substance concentrations used (high dose group 1/3 of LD50) did not show any mutagenic effect in cytogenetic analysis of mice bone marrow cells.
- Executive summary:
Two in vivo chromosomal aberration tests in mice analysed bone marrow cells were negative. A negative result was obtained after single oral doses up to 618 mg/kg (corresponding to 1/3 of LD50-value) at sampling times of 6 h, 12 h, and 24 h after single treatments. In addition negative results after repeated oral doses were also determined. Doses up to 618 mg/kg bw were given five times with intervals of 24 h; sampling time was 6 h after last administration. No information about clinical symptoms or cytotoxic effects is given by the authors. However, from the toxicokinetic data available it can be concluded that the substance was available at the target organ (see section 7.1). Positive controls revealed the expected effects and demonstrated the sensitivity of the test system.
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