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EC number: 404-450-2 | CAS number: 118685-34-0 COBRATEC 435
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 23.08.1988 - 12.09.1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Sodium 5-N-butylbenzotriazole
- EC Number:
- 404-450-2
- EC Name:
- Sodium 5-N-butylbenzotriazole
- Cas Number:
- 118685-34-0
- Molecular formula:
- CCCCc1ccc2c(c1)nnn2[Na]
- IUPAC Name:
- sodium 5-butyl-1H-1,2,3-benzotriazol-1-ide
- Details on test material:
- Name of test material: Butyl benzotriazole-sodium salt (referred to in the study as Butyl BZT)
Physical state: brown liquid
Purity: 37% aqueous solution
Strage condition: stored at room temperature
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1538
- Additional strain / cell type characteristics:
- other: Defective in DNA repair capacity (Δ uvrB), defective lipopolysaccharide barrier on the cell wall (rfa)
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Additional strain / cell type characteristics:
- other: Defective in DNA repair capacity (Δ uvrB), defective lipopolysaccharide barrier on the cell wall (rfa). Strains TA 98 and TA 100 also contain a resistance transfer factor (plasmid pKM 101)
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254 induced rat liver homogenate S-9
- Test concentrations with justification for top dose:
- Dose range finding test:
100, 10, 1, 0.1% Butyl benzotriazole-sodium salt 37% solution
Equivalent to 37000, 3700, 370, 37 µg/plate of Butyl benzotriazole-sodium salt
Mutation tests:
1, 0.3, 0.1, 0.03, 0.01% Butyl benzotriazole-sodium salt 37% solution
Equivalent to 370, 111, 37, 11.1, 3.7 µg/plate of Butyl benzotriazole-sodium salt - Vehicle / solvent:
- Solvent: Water
Controlsopen allclose all
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- With S-9 mix, at 2 µg/plate for strains TA 1535 and TA 1537, at 0.5 µg/plate for strains TA 1538 and TA 98, at 1 µg/plate for strain TA 100
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- Remarks:
- Without S-9 mix, at 5 µg/plate for strain TA 1535, at 3 µg/plate for strain TA 100
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- Without S-9 mix, at 80 µg/plate for strain TA 1537
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- Without S-9 mix, at 2 µg/plate for strain TA 1538, at 1 µg/plate for strain TA98
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- (A 1% stock solution was chosen as the top dose level in the mutation tests)
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- No substantial increases in revertant colony numbers of any of the five tester strains were observed following treatment with Butyl benzotriazole-sodium salt at any dose level, either in the presence or absence of metabolic activation (S-9 mix).
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
The revertant colony counts for Butyl benzotriazole-sodium salt obtained in the dose range finding test are shown in Table 1 of attached study report. Butyl benzotriazole-sodium salt was toxic towards the tester strains at the higher dose Ievels. Therefore a 1% stock solution was chosen as the top dose level in the mutation tests.
The mean number of revertant colonies, together with the individual plate counts for Butyl benzotriazole-sodium salt obtained in the flrst mutation test with tester strains TA 1535, TA 1537, TA 1538, TA 98 and TA 100 are shown in Table 2 of study report. Positive control mutability checks are shown in Table 3 of study report.
The mean number of revertant colonies, together with the individual plate counts for Butyl benzotriazole-sodium salt obtained in the second mutation test with tester strains TA 1535, TA 1537, TA 1538, TA 98 and TA 100 are shown in Table 4 of study report. Positive control mutabÍIity checks are shown in Table 5 of study report.
No substantial increases in revertant colony numbers of any of the five tester strains were observed following treatment with Butyl benzotriazole-sodium salt at any dose level, either in the presence or absence of metabolic activation (S-9 mix).
It is'concluded that no evidence of mutagenic potential of Butyl benzotriazole-sodium salt was obtained in this bacterial test system at the dose levels used. - Executive summary:
In this in vitro assessment of the mutagenic potential of Butyl benzotriazole-sodium salt, Slamonella typhimurium (strains TA 1535, TA1537, TA1538, TA 98 and TA 100) were exposed to the test substance, diluted in water whixh was also used as negatice control.
Tests were performed, in presence and abscence of metabolic activation system (Aroclor 1254 induced rat liver homogenate S-9). In the preliminary toxicity test, toxicity was observed at dose levels upper than 370 µg/plate of Butyl benzotriazole-sodium salt (1% of Butyl benzotriazole-sodium salt 37% solution). A top dose level of 370 µg/plate of Butyl benzotriazole-sodium salt was chosen for the mutation study. Other dose levels used in the mutation assays were 111, 37, 11.1 and 3.7 µg/plate of Butyl benzotriazole-sodium salt (respectively 0.3, 0.1, 0.03 and 0.01% of Butyl benzotriazole-sodium salt 37% solution)
No evidence of mutagenic activity was seen at any dose level of Butyl benzotriazole-sodium salt ineither mutation test.
The concurrent positive control compounds demonstrated the sensitivity of the assay and the the metabolising activity of the liver preparation.
It is'concluded that no evidence of mutagenic potential of Butyl benzotriazole-sodium salt was obtained in this bacterial test system at the dose levels used.
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