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EC number: 221-221-0 | CAS number: 3033-77-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004-08-11 to 1004-09-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to the appropriate OECD test guideline, and in compliance with GLP. Analytical monitoring was also conducted.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 004
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,3-epoxypropyltrimethylammonium chloride
- EC Number:
- 221-221-0
- EC Name:
- 2,3-epoxypropyltrimethylammonium chloride
- Cas Number:
- 3033-77-0
- Molecular formula:
- C6H14NO.Cl
- IUPAC Name:
- N,N,N-trimethyl(oxiran-2-yl)methanaminium chloride
- Details on test material:
- - Name of test material (as cited in study report): 2,3-Epoxypropyltrimethylammonium chloride / 72.5 % and GMAC (Glycidyl trimethyl ammonium chloride).
- Physical state: yellowish, clear liquid
- Storage condition of test material: refrigerator, 7 +/- 2 degC, protected from moisture and light.
The results are expressed on the basis of the active ingredient.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The concentrations of 2,3-Epoxypropyltrimethylammonium chloride (EPTAC) were determined in all test item concentrations and controls on days 0, 7, 14, 19 (fresh media) and days 2, 9, 16, 21 (old media).
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: a stock solution of 100 mg/L, i.e. 72.5 mg a.i./L was agitated to aid dispersion. No other information is reported. It is assumed by the reviewer that a similar method of dispersion was used to achieve test concentrations from serial dilutions of the stock solution or by adding the adequate amounts of the stock solution to achieve nominal concentrations in the test vessels.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Clone: 5
- Source: the test organisms were bred at the testing facility, origin Insitut fur Wasser-, Boden- und Lufthygiene (WaBoLu).
- Age of parental stock (mean and range, SD): 2-24 h old, the test organisms were not the first brood progeny.
- Feeding during test
- Food type and amount: The food quantity was a D. subspicatus (0.395 - 1.45 mL) and C. vulgaris (0.375- 0.745 mL) suspension of algae per test vessel (corresponding to 0.1 - 0.2 mg C per Daphnia per day). lt varied according to the growth of the daphnids, but was the same for all test groups on any particular day.
- Frequency: At lest 5 times per week.
ACCLIMATION
- Acclimation conditions (same as test or not): the test organisms were bread in an incubator with similar conditions to the test (temperature, illumination and photoperiod), in 2-3 L glass vessels with approx. 1.8 L culture medium.
- Type and amount of food: a mix of D. subspicatus and C. vulgaris with algae cell density of >10^6 cells/ml ad libitum.
- Feeding frequency: at least 5x per week
- Health during acclimation: the stock is defined as healthy by the authors of the report.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- > 140 mg/L as CaCO3
- Test temperature:
- range: 20.3 - 21.9 °C
- pH:
- pH range: 7.7-8.6 control, 7.8-8.5 0.48 mg a.i./L, 8.4-9.0 4.35 mg a.i./L.
- Dissolved oxygen:
- The final oxygen concentrations of the measured replicates in the test media of nominal 0.49, 4.35 mg a.i./L and the control were found to be greater than or equal to 7.66 mg/L.
- Nominal and measured concentrations:
- Nominal concentrations: 0 .05, 0.16, 0.49, 1.45, 4.35 mg a.i./L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: glass beakers (5 (ID) x 8 (h) cm), 100 ml filled with 50 ml test solution.
- Renewal rate of test solution (frequency): 3 times per week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4, according to Elendt (1990), modified to a total hardness of 160-180 mg/L as CaCO3
- Total organic carbon: 1.44 mg/L
- Particulate matter: <0.1 mg/L
- Metals: within acceptable ranges
- Pesticides: <0.025 ug/L
- Chlorine: <0.003 mg/L
- Conductivity: <0.1 uOhm
- Culture medium different from test medium: culture medium - Elendt M4, according to Elendt (1990), modified to a total hardness of 160-180 mg CaCO3/L
- Intervals of water quality measurement: Dissolved oxygen concentration, pH, total hardness and water temperature were measured once a week, in fresh and old media, in one replicate of the control and the highest concentration of the test item.
OTHER TEST CONDITIONS
- Photoperiod: 16h/8h light/dark cycle
- Light intensity: max 20 uE x m^2/s
EFFECT PARAMETERS MEASURED:
o mortality of parent daphnia - measured once per day
o newborn offspring first appearance - daily;
o number of live and dead offspring - daily;
o aborted eggs - daily;
o abnormalities of parent daphnia - each day of observation;
o body length and weight (parent daphnia) - end of test.
RANGE-FINDING STUDY
- Test concentrations: two separate range finding studies: 0.1-100 mg/L and 2.5-10 mg/L
- Results used to determine the conditions for the definitive study: 100 and 30% immobilisation at 100 and 10 mg/L in the first test, while 10% immobilisation was achieved at 10 mg/L in the second test. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.49 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.55 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Remarks on result:
- other: 96% CL 0.47-0.66 mg/L
- Details on results:
- - Mortality of parent animals: At 1.45 and 4.35 mg a.i./L the immobilisation of parental amounted to 100% at 0.49 mg a.i./L to 50 % after 21 days.
- No. of offspring produced: 0.49 mg a.i./L treatment showed a significantly reduced number of offspring compared to the control.
- Body length and weight of parent animals: body length at the 0.49 mg a.i./L treatment level was significantly reduced compared to the control.
- Time to first brood release or time to hatch: no statistical difference between control and treatments reported.
- Brood size: the average number of juveniles per parent in the control group was 108 after 21 days. At 0.49 mg/L the number of juveniles per parent was statistically significantly reduced. - Results with reference substance (positive control):
- - Results with reference substance valid? yes, according to DIN 38412 L 30 the validity range is 1.0-2.5 mg/L during a 24 h exposure.
- Relevant effect levels: EC50 after 24h was 1.94 mg/L
- Other: - Reported statistics and error estimates:
- The EC50 value and CI were calculated by probit analysis. The NOEC was calculated by Kruskal-Wallis ANOVA on Ranks, DUNN's method.
Any other information on results incl. tables
Table 1: Effect of test material on growth and survival of D. magna (average per 10 replicates unless otherwise stated) during the 21 day test.
Treatment (mg a.i./L) nominal concentration |
Mortality of parents (dead or immobile) |
Total no. of offspring |
No. of juveniles per parent alive (± SD) |
Day of first appearance of juveniles |
Mean length (mm) |
Mean weight (mg) |
|
No Dead (per 10 replicates) |
% (per 10 replicates) |
||||||
Control |
1 |
10 |
970 |
108 ± 5.0 |
7.1 |
5.39 |
1.27 |
4.35 |
10 |
100 |
- |
0 ± 0 |
- |
- |
- |
1.45 |
10 |
100 |
- |
0 ± 0 |
7.9 |
- |
- |
0.49 |
5 |
50 |
365 |
73 ± 15.5 |
8.0 |
4.6 |
0.22 |
0.16 |
1 |
10 |
961 |
107 ± 14.1 |
7.7 |
5.33 |
0.68 |
0.05 |
0 |
0 |
1025 |
103 ± 3.8 |
7.1 |
5.35 |
1.05 |
- denotes when an endpoint is not applicable, e.g. the parent daphnia have died before the first appearance of juveniles.
Table 2: Parental daphnia immobilisation results.
Endpoint |
Result (mg a.i./L, nominal) |
NOEC |
0.16 |
LOEC |
0.49 |
EC50 |
0.34 (0.27-0.42 95% CL) |
Table 3: Nominal and measured concentrations during 21 days.
|
Measured concentration (mg a.i./L) results |
|||||
Nominalconcentr. |
0 (control) |
4.35 |
1.45 |
0.49 |
0.16 |
0.05 |
Range (min.-max.) |
< LOQ |
4.72 |
1.5-1.54 |
0.48-0.57 |
0.161-0.186 |
0.051-0.058 |
The highest treatment level is only presented as one measurement because the day 0 and 2 values are reported as an average and thereafter the organisms perished.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 21 day NOEC value of 0.16 mg a.i./l has been determined for the effects of the test substance on reproduction of D. magna during a 21 day chronic test.
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