Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 265-182-8 | CAS number: 64742-79-6 A complex combination of hydrocarbons obtained from a petroleum stock by treating with hydrogen to convert organic sulfur to hydrogen sulfide which is removed. It consists predominantly of hydrocarbons having carbon numbers predominantly in the range of C13 through C25 and boiling in the range of approximately 230°C to 400°C (446°F to 752°F).
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / bone marrow chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1985
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 475 (Mammalian Bone Marrow Chromosome Aberration Test)
- GLP compliance:
- not specified
- Type of assay:
- chromosome aberration assay
Test material
- Reference substance name:
- 64742-80-9
- Cas Number:
- 64742-80-9
- IUPAC Name:
- 64742-80-9
- Reference substance name:
- Hydrodesulfurised middle distillate (CAS# 64742-80-9)
- IUPAC Name:
- Hydrodesulfurised middle distillate (CAS# 64742-80-9)
- Test material form:
- other: low viscosity liquid hydrocarbon
- Details on test material:
- - Hydrodesulphurised middle distillate
- Sample API 81-09
- Name of test material (as cited in study report): API 81-09 (CAS# 64742-80-9)
- Physical state: clear, pale straw coloured liquid
Constituent 1
Constituent 2
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
Administration / exposure
- Route of administration:
- intraperitoneal
- Duration of treatment / exposure:
- single IP dose. Phase dsacrifice at 6, 24 and 48 hrs post dose
- Frequency of treatment:
- single dose
- Post exposure period:
- 6, 24 or 48 hrs post dose
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0.3, 1.0 and 3.0 g/kg
Basis:
nominal conc.
- No. of animals per sex per dose:
- 5 animals per dose group for each phased sacrifice time
Examinations
- Tissues and cell types examined:
- harvested bone marrow cells
- Details of tissue and slide preparation:
- bone marrow cells harvested 3 hrs after colchicine administration. Slides satined with Geisma
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
There was no evidence of a treament-related effect.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
There was no evidence of a treatment related genetic toxicity effect. - Executive summary:
Three groups of 5 Sprague Dawley rats of each sex were given a single dose test material (3, 1 and 0.3 g/kg) intraperitoneally. One group of each treatment and sex was killed 6, 24 and 48 hours after treatment, having been injected with colchicine two or three hours previously. The bone
marrow was removed, fixed, stained with Giemsa and examined for chromosomal aberrations. Negative and positive control groups were also included. There was no evidence of a treatment related genetic toxicity effect.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
