Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 232-395-2 | CAS number: 8013-75-0 A combination of amyl alcohols, primarily isoamyl alcohol and 2-methyl-1-butanol. Other alcohols, acids, esters and aldehydes may also be present.
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
EC3/10 = 65 - 470 mg/L
EC50 > 1000 mg/L
Key value for chemical safety assessment
Additional information
Studies on the toxicity to microorganisms are available for two of the four main constituents of Fusel oil.
The key study available for 2 -Methylbutanol (CAS No. 137-32-6) was conducted according to the OECD Guideline 209 under GLP conditions (BASF, 2010). Activated sludge collected from a domestic sewage treatment plant was exposed to nominal test concentrations of 0, 62.5, 125, 250, 500 and 1000 mg/L in a dose-response test over 180 min. A positive control (3,5-dichlorophenol) was used in order to validate the test conditions. On the basis of respiration rate the effect concentrations were determined by measuring the CO2-evolution. At the end of the exposure period an EC50-value could not be calculated and it is considered to be > 1000 mg/L (nominal). The EC10 (180 min) was calculated to be 470 mg/L.
For Ethanol (CAS No. 64-17-5), a number of studies have been carried out to assess the toxicity of ethanol to microorganisms. In those studies ethanol has been tested on different freshwater protozoa and bacteria species. The study available forPseudomonas putida,which was performed according to DIN 38412, part 8 was chosen as key study (Bringman and Kuhn, 1978, 1980). In the dose-response test 14 concentrations were used which were not further specified. After a test duration of 72 h the toxicity threshold or EC3 was reported to be approximately 65 mg/L.
No studies on the toxicity to microorganisms are available for 3-Methylbutanol (CAS No. 123-51-3) and 2-Methylpropanol (CAS No. 78-83-1). 3 -Methylbutanol is structurally very similar to 2-Methylbutanol; both are branched primary aliphatic alcohols with five carbons. The structure of 2 -Methylpropanol only differs from 2 -Methylbutanol by the one carbon shorter chain length. The ecotoxicological profile is thus expected to be similar for the three constituents and thus effects of Fusel oil to microorganisms are not expected.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
